Colocalization of ferroportin‐1 with hephaestin on the basolateral membrane of human intestinal absorptive cells

Journal of Cellular Biochemistry - Tập 101 Số 4 - Trang 1000-1010 - 2007
Okhee Han1, Eun‐Young Kim2
1Department of Nutritional Sciences, S‐126 Henderson Building South, Pennsylvania State University, University Park, PA 16802.
2Department of Nutritional Sciences, Pennsylvania State University, University Park, Pennsylvania 16802

Tóm tắt

AbstractAn iron exporter ferroportin‐1 (FPN‐1) and a multi‐copper oxidase hephaestin (Heph) are predicted to be expressed on the basolateral membrane of the enterocyte and involved in the processes of iron export across the basolateral membrane of the enterocyte. However, it is not clear where these proteins are exactly located in the intestinal absorptive cell. We examined cellular localization of FPN‐1 and Heph in the intestinal absorptive cells using the fully differentiated Caco‐2 cells. Confocal microscope study showed that FPN‐1 and Heph are located on the basolateral membrane and they are associated with the transferrin receptor (TfR) in fully differentiated Caco‐2 cells grown on microporous membrane inserts. However, Heph protein was not detected in the crypt cell‐like proliferating Caco‐2 cell. In stably transfected human intestinal absorptive cells expressing human FPN‐1 modified by the addition of GFP at the C‐terminus, we show that FPN‐1‐GFP is located on the basolateral membrane and it is associated with Heph suggesting the possibility that FPN‐1 might associate and interact with Heph in the process of iron exit across the basolateral membrane of intestinal absorptive cell. J. Cell. Biochem. 101: 1000–1010, 2007. © 2007 Wiley‐Liss, Inc.

Từ khóa


Tài liệu tham khảo

10.1074/jbc.M000713200

Bannerman RM, 1976, Genetic defects of iron transport, Fed Proc, 35, 2281

Bissonnette M, 1994, 1,25(OH)2 vitamin D3 activates PKC‐alpha in Caco‐2 cells: A mechanism to limit secosteroid‐induced rise in [Ca2+]i, Am J Physiol, 267, G465

10.1152/ajpgi.00227.2005

10.1182/blood-2003-09-3139

De Domenico I, 2006, Molecular and clinical correlates in iron overload associated with mutations in ferroportin, Haematologica, 91, 1092

10.1074/jbc.270.3.1098

10.1182/blood-2001-11-0132

10.1038/35001596

10.1016/j.cmet.2005.01.003

Glahn RP, 1996, Caco‐2 cell iron uptake from meat and casein digests parallels in vivo studies: Use of a novel in vitro method for rapid estimation of iron bioavailability, J Nutr, 126, 332, 10.1093/jn/126.1.332

10.1042/BJ20051682

10.1152/ajpgi.00414.2001

Han O, 1995, Reduction of Fe(III) is required for uptake of nonheme iron by Caco‐2 cells, J Nutr, 125, 1291

10.1152/ajpgi.1998.275.3.G534

10.1073/pnas.96.19.10812

10.1016/S0092-8674(04)00343-5

10.1074/jbc.M301988200

10.1073/pnas.0409409102

10.1136/gut.2003.019026

10.1016/j.bcmd.2005.04.005

10.1146/annurev.cb.08.110192.001105

10.1016/S1097-2765(00)80425-6

10.1172/JCI200113468

10.1074/jbc.M401151200

10.1038/90038

10.1152/ajpcell.1994.267.6.C1582

Osaki S, 1971, The mobilization of iron from the perfused mammalian liver by a serum copper enzyme, ferroxidase I, J Biol Chem, 246, 3018, 10.1016/S0021-9258(18)62284-7

10.1242/jcs.105.2.461

10.1002/path.1700950118

10.1074/jbc.M512042200

10.1126/science.271.5255.1552

10.1093/protein/15.3.205

10.1016/S0378-4274(01)00363-0

10.1038/5979

Thông tin
Thông tin xuất bản

Journal of Cellular Biochemistry

Tập 101 Số 4

1000-1010

Thông tin tác giả