Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd

American Association for the Advancement of Science (AAAS) - Tập 269 Số 5223 - Trang 496-512 - 1995
Robert Fleischmann1, Mark D. Adams1, Owen White1, Rebecca A. Clayton1, Ewen F. Kirkness1, Anthony R. Kerlavage1, Carol J. Bult1, Jean-François Tomb2, Brian Dougherty2, Joseph M. Merrick3, Keith McKenney4, David Sturgill1, Will FitzHugh1, Chris Fields1, Jeannine D. Gocayne1, John Scott1, Robert B Shirley1, Li-lng Liu1, Anna Glodek1, Jenny M. Kelley1, Janice Weidman1, Carolyn S. Phillips1, Tracy Spriggs1, Eva Hedblom1, Matthew Cotton1, Teresa R. Utterback1, Michael C. Hanna1, D.T. Nguyen1, Deborah M. Saudek1, Rhonda Brandon1, Leonard Fine1, Janice L. Fritchman1, Joyce L. Fuhrmann1, N. S. M. Geoghagen1, Cheryl L. Gnehm1, Lisa McDonald1, Keith V. Small1, Claire M. Fraser1, Hamilton O. Smith2, J. Craig Venter1
1The Institute for Genomic Research (TIGR), Gaithersburg, MD, 20878, USA.
2Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
3State University of New York, Department of Microbiology, Buffalo, NY, 14214, USA.
4National Institute for Standards and Technology, Gaithersburg, MD 20878, USA.

Tóm tắt

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.

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American Association for the Advancement of Science (AAAS)

Tập 269 Số 5223

496-512

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