Direct detection of two cross‐reactive antigens between porcine and rabbit zonae pellucidae by radioimmunoassay and immunoelectrophoresis

Wiley - Tập 217 Số 3 - Trang 423-433 - 1981
David M. Wood1, Bonnie S. Dunbar1
1The Population Council, Center for Biomedical Research, New York, New York, 10021

Tóm tắt

AbstractZonae pellucidae were isolated from oocytes obtained from rabbit and porcine ovaries. Antisera were prepared in rabbits against heat‐solubilized porcine zonae and in guinea pigs against intact and heat‐solubilized rabbit zonae and against purified rabbit zona proteins isolated from two‐dimensional electro‐phoresis gels. These antisera were used in radioimmunoassay studies to show cross‐reactivity between porcine and rabbit zona antigens. Immunoelectrophoresis studies also demonstrated the immunochemical cross‐reactivity between two distinct antigens of rabbit and porcine zonae. These two rabbit zona antigens were demonstrated to be immunochemically identical to porcine zona antigens by crossed‐line immunoelectrophoresis.

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Tài liệu tham khảo

10.1073/pnas.74.12.5421

10.1016/0003-2697(78)90229-4

10.1016/0003-2697(78)90230-0

Anderson N. G. N. L.Anderson andS. L.Tollaksen(1979)Operation of the ISO‐DALT system. ANL‐BIM‐79‐2 Ar‐gonne Illinois.

10.1016/S0003-2697(79)80157-8

Dunbar B. S., 1980, Model systems to study the relationship between antibodies to zonae and infertility. Comparison of rabbit and porcine zona pellucidae, 9th International Congress on Animal Reproduction and Artifical Insemination, 191

10.1095/biolreprod22.4.941

Dunbar B. S., 1980, Comparison of the macromolecular structure of the zona pellucida of porcine and rabbit oocytes, J. Cell Biol., 87, 143a

10.1021/bi00543a017

Dunbar B. S. C.Liu andD. W.Sammons(1981) Identification of the three major proteins of porcine and rabbit zonae pellucidae by high resolution two‐dimensional gel electrophoresis: Comparison with serum follicular fluid and ovarian cell proteins.Biol. Reprod.(in press).

10.1016/0165-0378(81)90029-2

10.1002/mrd.1120010105

10.1016/S0015-0282(16)42744-5

10.1530/jrf.0.0420377

Kessler S. W., 1975, Rapid isolation of antigens from cells with Staphylococcal protein A‐antibody adsorbent; parameters of the interaction of antibody‐antigen complexes with protein A, Immunol., 115, 1617, 10.4049/jimmunol.115.6.1617

Kessler S. W., 1976, Cell membrane antigen isolation with Staphylococcal protein A‐antibody adsorbent, J. Immunol., 117, 1482, 10.4049/jimmunol.117.5_Part_1.1482

Krøll J., 1973, A Manual of Quantitative Immunoelectrophoresis. Methods and Applications, 47

Krøll J., 1973, A Manual of Quantitative Immunoelectrophoresis. Methods and Applications, 79

10.1007/BF01947334

10.1016/S0015-0282(16)44118-X

10.1530/jrf.0.0450487

10.1095/biolreprod21.3.709

10.1095/biolreprod16.2.164

10.1530/jrf.0.0510165

10.1126/science.70076

10.1095/biolreprod14.3.354

10.1002/jez.1401950307

10.1530/jrf.0.0540233

Tsunoda Y., 1977, Inhibition of fertilization in mice by anti‐zona pellucida antiserum, Jpn. J. Zootech. Sci., 48, 784

Weeke B., 1973, A Manual of Quantitative Immunoelectrophoresis. Methods and Applications, 15

Weeke B., 1973, A Manual of Quantitative Immunoelectrophoresis. Methods and Applications, 37

Weeke B., 1973, Methods and Applications. (Scand. J. Immunol., 47

Wood S. M. C.Liv andB. S.Dunbar(1981) The effect of alloimmunization and heteroimmunization with zonae pellucidae on fertility in rabbits.Biol. Reprod.(in press).