Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

American Association for the Advancement of Science (AAAS) - Tập 318 Số 5858 - Trang 1917-1920 - 2007
Junying Yu1,2,3,4,5, Maxim A. Vodyanik1,2,6,4,5, Kim Smuga-Otto1,2,6,4,5, Jessica Antosiewicz‐Bourget1,2,6,4,5, Jennifer L. Frane1,2,6,4,5, Shulan Tian1,2,6,4,5, Jeff Nie1,2,6,4,5, Guðrún A. Jónsdóttir1,2,6,4,5, Victor Ruotti1,2,6,4,5, Ron Stewart1,2,6,4,5, Igor I. Slukvin1,2,6,4,5, James A. Thomson1,2,6,4,5
1Department of Anatomy, University of Wisconsin-Madison, Madison, WI 53706–1509, USA.
2Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, Madison, WI, 53706, USA
3Genome Center of Wisconsin, Madison, WI 53706-1580, USA. [email protected]
4WiCell Research Institute, Madison, WI 53707–7365, USA.
5Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715–1299, USA.
6Genome Center of Wisconsin, Madison, WI 53706–1580, USA.

Tóm tắt

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors ( OCT4, SOX2, NANOG , and LIN28 ) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.

Từ khóa


Tài liệu tham khảo

10.1038/385810a0

10.1016/j.stem.2007.05.014

10.1038/nature05934

10.1016/j.cell.2006.07.024

10.1038/nature05944

10.1038/sj.onc.1210353

10.1634/stemcells.2005-0292

10.1038/nbt788

10.1182/blood-2004-04-1649

Materials and methods are available as supporting material on Science Online.

10.1038/nature04914

10.1242/dev.02286

10.1038/nature05874

10.1126/science.282.5391.1145

10.1038/nbt1335

We thank the Charlotte Geyer Foundation for their support. Other funding included NIH grants P51 RR000167 and P20 GM069981. We thank K. J. Heidarsdottir B. K. Gisladottir M. Probasco and C. Glennon for technical assistance and D. J. Faupel for critical reading of the manuscript. The authors declare competing financial interests. J.A.T. owns stock serves on the Board of Directors and serves as Chief Scientific Officer of Cellular Dynamics International and Stem Cell Products. J.A.T. also serves as Scientific Director of the WiCell Research Institute. Microarray data have been deposited in the Gene Expression Omnibus (GEO) database (accession number GSE9164).

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 318 Số 5858

1917-1920

Thông tin tác giả