Cadmium-Resistant <i>Pseudomonas putida</i> Synthesizes Novel Cadmium Proteins

American Association for the Advancement of Science (AAAS) - Tập 225 Số 4666 - Trang 1043-1046 - 1984
Denise P. Higham1, Peter J. Sadler1, Michael D. Scawen2
1Department of Chemistry, Birkbeck College, University of London, London WC1E 7HX, United Kingdom
2Microbial Technology Laboratory, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, SP4 0JG, United Kingdom

Tóm tắt

Three cysteine-rich proteins of molecular weight 4000 to 7000, containing 4 to 7 gram atoms of cadmium, zinc, and copper per mole were isolated from Pseudomonas putida growing in 3 m M cadmium. The three proteins were induced during different phases of growth, and the smallest (molecular weight 3600; 3 gram atoms of cadmium) was released into the medium when the cells lysed. The results of amino acid analyses and of ultraviolet, circular dichroism, electron paramagnetic resonance, and cadmium-113 nuclear magnetic resonance spectroscopy suggest a novel cadmium(II)-zinc(II)-copper(I) cluster structure for the major protein.

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American Association for the Advancement of Science (AAAS)

Tập 225 Số 4666

1043-1046

Thông tin tác giả