Subunit organisation and symmetry of pore‐forming, oligomeric pneumolysin

FEBS Letters - Tập 371 - Trang 77-80 - 1995
Peter J. Morgan1, Stefan C. Hyman2, Arthur J. Rowe2, Timothy J. Mitchell1, Peter W. Andrew1, Helen R. Saibil3
1Department of Microbiology and Immunology, University of Leicester, Leicester, LE1 9HN, UK
2NCMH, Department of Biochemistry, University of Leicester, Leicester, LE1 7RH, UK
3Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, UK

Tóm tắt

We present a detailed analysis of the oligomeric subunit organisation of pneumolysin by the use of negative stain electron microscopy and image processing to produce a projection density map. Analysis of the rotational symmetry has revealed a large and variable subunit number, between 40–50. The projected subunit density by rotational averaging shows at least two distinct subunit domains at different radial positions. Side views of the rings reveal further details concerning the dimensions of the oligomer in the membrane. On the basis of these observations and our previous knowledge of the monomer domain structure we propose that the 4‐domain subunits are packed in a square planar arrangement to form the pneumolysin oligomer.

Tài liệu tham khảo

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FEBS Letters

Tập 371

77-80

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