Cytosolic thioredoxin peroxidase I is essential for the antioxidant defense of yeast with dysfunctional mitochondria

FEBS Letters - Tập 509 - Trang 430-434 - 2001
Ana P.D. Demasi1, Gonçalo A.G. Pereira2, Luis E.S. Netto3
1Departamento de Bioquı́mica, IB, UNICAMP, 13083-970 Campinas, SP, Brazil
2Departamento de Genética e Evolução, IB, UNICAMP, 13083-970 Campinas, SP, Brazil
3Departamento de Biologia, IB, USP, 05508-900 São Paulo, SP, Brazil

Tóm tắt

The specific role of cytosolic thioredoxin peroxidase I (cTPx I), encoded by TSA1 (thiol‐specific antioxidant), was investigated in the oxidative stress response of Saccharomyces cerevisiae. In most cases, deletion of TSA1 has showed only a slight effect on hydrogen peroxide sensitivity. However, when the functional state of the mitochondria was compromised, the necessity of TSA1 in cell protection against this oxidant was much more evident. All the procedures used to disrupt the mitochondrial respiratory chain promoted increases in the generation of H2O2 in cells, which could be related to their elevated sensitivity to oxidative stress. In fact, TSA1 is highly expressed when cells with respiratory deficiency are exposed to H2O2. In conclusion, our results indicate that cTPx I is a key component of the antioxidant defense in respiratory‐deficient cells.

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FEBS Letters

Tập 509

430-434

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