Beneficial effects of melatonin on in vitro embryo production from juvenile goat oocytes

Reproduction, Fertility and Development - Tập 30 Số 2 - Trang 253 - 2018
Sandra Soto‐Heras1, M. Roura1, M. G. Catalá1, Irene Menéndez‐Blanco1, D. Izquierdo1, Ali A. Fouladi‐Nashta2, M. T. Paramio1
1Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Travessera dels Turons s/n, 08193 Bellaterra, Barcelona, Spain.
2Reproduction Genes and Development Group, Department of Veterinary Basic Sciences, The Royal Veterinary College, Hawkshead Lane, Hatfield, Herts AL97TA, UK.

Tóm tắt

Melatonin is a universal antioxidant that improves in vitro embryo production in several species. The aims of this study were to determine the melatonin concentration in the ovarian follicular fluid (FF) of juvenile goats and the effect of melatonin during in vitro maturation (IVM) on embryo development. The FF melatonin concentration was 0.57­–1.07 × 10−9 M, increasing with follicular diameter. Oocytes were matured, fertilised and cultured under conventional conditions. Blastocyst development, embryo quality and levels of reactive oxygen species (ROS) and reduced glutathione were assessed. In Experiment 1 different melatonin concentrations (10−3, 10−7, 10−9, 10−11 M) were added to the IVM medium, which contained cysteamine as antioxidant, and no differences were observed. In Experiment 2, melatonin (10−7 M) was tested in the presence or absence of cysteamine (experimental groups: melatonin, cysteamine, melatonin + cysteamine, non-antioxidant). The melatonin group presented a higher blastocyst rate than the non-antioxidant group (28.9 vs 11.7%; P < 0.01) and a higher total cell number than the cysteamine group (225.1 vs 129.0; P < 0.05). Oocytes from the melatonin and cysteamine groups had lower ROS levels than those from the non-antioxidant group. This study shows that melatonin is an interesting tool for improving oocyte competence in juvenile goats as it increases embryo production and quality.

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Tài liệu tham khảo

Bahadori, 2013, Iran. J. Reprod. Med., 11, 11

Banerjee, 2012, J. Pineal Res., 53, 122, 10.1111/j.1600-079X.2012.00977.x

Barros, 2013, Reprod. Domest. Anim., 48, 1025, 10.1111/rda.12209

Casao, 2010, Span. J. Agric. Res., 8, 35, 10.5424/sjar/2010081-1141

Catalá, 2011, Reproduction, 142, 517, 10.1530/REP-10-0528

Chattoraj, 2005, Gen. Comp. Endocrinol., 140, 145, 10.1016/j.ygcen.2004.10.013

Cruz, 2014, Anim. Reprod. Sci., 145, 150, 10.1016/j.anireprosci.2014.01.011

Deleuze, 2010, Reprod. Domest. Anim., 45, e476, 10.1111/j.1439-0531.2010.01587.x

Devine, 2012, Biol. Reprod., 86, 27, 10.1095/biolreprod.111.095224

El-Raey, 2011, Mol. Reprod. Dev., 78, 250, 10.1002/mrd.21295

Franco, 2009, Cell Death Differ., 16, 1303, 10.1038/cdd.2009.107

Goud, 2008, Free Radic. Biol. Med., 44, 1295, 10.1016/j.freeradbiomed.2007.11.014

Guérin, 2001, Hum. Reprod. Update, 7, 175, 10.1093/humupd/7.2.175

He, 2016, Int. J. Mol. Sci., 17, 939, 10.3390/ijms17060939

Holm, 1999, Theriogenology, 52, 683, 10.1016/S0093-691X(99)00162-4

Jiao, 2013, PLoS One, 8, e58018, 10.1371/journal.pone.0058018

Kang, 2009, J. Pineal Res., 46, 22, 10.1111/j.1600-079X.2008.00602.x

Koeman, 2003, Theriogenology, 60, 879, 10.1016/S0093-691X(03)00090-6

Korkmaz, 2012, Gene, 503, 1, 10.1016/j.gene.2012.04.040

Lane, 1997, J. Reprod. Fertil., 109, 153, 10.1530/jrf.0.1090153

Leoni, 2009, Reprod. Fertil. Dev., 21, 901, 10.1071/RD09015

Leoni, 2015, PLoS One, 10, e0124911, 10.1371/journal.pone.0124911

Li, 2016, J. Anim. Sci. Biotechnol., 7, 33, 10.1186/s40104-016-0093-9

Lord, 2013, Biol. Reprod., 88, 67, 10.1095/biolreprod.112.106450

Morton, 2008, Reprod. Domest. Anim., 43, 137, 10.1111/j.1439-0531.2008.01153.x

Nikmard, 2017, Anim. Sci. J., 88, 586, 10.1111/asj.12675

Paramio, 2014, Reprod. Domest. Anim., 49, 37, 10.1111/rda.12334

Park, 2014, Reprod. Domest. Anim., 49, 370, 10.1111/rda.12281

Parrish, 1986, Theriogenology, 25, 591, 10.1016/0093-691X(86)90143-3

Reiter, 2009, Biol. Reprod., 81, 445, 10.1095/biolreprod.108.075655

Reiter, 2016, J. Pineal Res., 61, 253, 10.1111/jpi.12360

Remião, 2016, Reprod. Toxicol., 63, 70, 10.1016/j.reprotox.2016.05.016

Rocha, 2013, Domest. Anim. Endocrinol., 44, 1, 10.1016/j.domaniend.2012.07.001

Rodrigues-Cunha, 2016, Theriogenology, 86, 1685, 10.1016/j.theriogenology.2016.05.026

Rodríguez-González, 2003, Mol. Reprod. Dev., 65, 446, 10.1002/mrd.10316

Romaguera, 2010, Theriogenology, 74, 364, 10.1016/j.theriogenology.2010.02.019

Sakaguchi, 2013, Reprod. Fertil. Dev., 25, 674, 10.1071/RD12091

Salimi, 2014, Cell J., 15, 294

Sampaio, 2012, Reprod. Biol. Endocrinol., 10, 103, 10.1186/1477-7827-10-103

Shi, 2009, J. Pineal Res., 47, 318, 10.1111/j.1600-079X.2009.00717.x

Song, 2016, Sci. Rep., 6, 35165, 10.1038/srep35165

Sugden, 1989, Experientia, 45, 922, 10.1007/BF01953049

Takada, 2012, Res. Vet. Sci., 92, 124, 10.1016/j.rvsc.2010.11.004

Takahashi, 2003, Mol. Reprod. Dev., 66, 143, 10.1002/mrd.10341

Tamura, 2009, Fertil. Steril., 92, 328, 10.1016/j.fertnstert.2008.05.016

Tamura, 2012, J. Ovarian Res., 5, 5, 10.1186/1757-2215-5-5

Tamura, 2013, Endocr. J., 60, 1, 10.1507/endocrj.EJ12-0263

Thouas, 2001, Reprod. Biomed. Online, 3, 25, 10.1016/S1472-6483(10)61960-8

Tian, 2014, J. Pineal Res., 57, 239, 10.1111/jpi.12163

Tomás-Zapico, 2005, J. Pineal Res., 39, 99, 10.1111/j.1600-079X.2005.00248.x

Tripathi, 2011, Eur. J. Pharmacol., 667, 419, 10.1016/j.ejphar.2011.06.005

Yuan, 2012, Biol. Reprod., 87, 78, 10.1095/biolreprod.112.099952

Zarazaga, 2010, Theriogenology, 74, 221, 10.1016/j.theriogenology.2010.02.005

Thông tin
Thông tin xuất bản

Reproduction, Fertility and Development

Tập 30 Số 2

253

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