Mitigating alcohol-induced neurohepatotoxicity in male albino rats with avocado and mustard

Aishah Abdullah AL-Qahtani1, Ali Abdullah Shati1, Amin A. Al-Doaiss1,2, Fahmy Gad Elsaid1,3
1Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia
2Anatomy and Histology Department, Faculty of Medicine, Sana’a University, Sana’a, Republic of Yemen
3Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt

Tóm tắt

Alcohol abuse is dangerous to one’s health and contributes to disorders such as neurotoxicity and hepatotoxicity worldwide. Twenty-five male albino rats, each weighing 200 ± 10 g, were divided into five groups. For 4 weeks, rats in the control group were given only a regular chow diet and ad libitum. Rats in the alcohol group received an oral gavage of alcohol at a dose of 40 mg/kg body weight each day. Rats in the alcohol and avocado extract group received oral gavage doses of 250 mg/kg b. wt./day of avocado extract and 40 mg/kg b. wt. of alcohol. Rats in the alcohol and mustard seed extract group received oral gavage doses of 250 mg/kg/day of mustard seed extract and 40 mg/kg/day of alcohol, respectively. Rats were given alcohol and a mixture of avocado and mustard seed extract at a dose of 250 mg/kg body weight each day by oral gavage for 4 weeks. Our results showed increased levels of hydrogen peroxide (H2O2) and lipid peroxidation in the liver and brain tissues, decreased glutathione content, glutathione peroxidase, superoxide dismutase, catalase, and glutathione-S-transferase. In rats consumed excessive alcohol, there was an increase in the activity of the tumor marker α-l-fucosidase in sera. Additionally, the liver and brain tissues of the alcoholic group showed decreased cytochrome c oxidase activity. Furthermore, changes in the expression levels of the genes for brain α-secretase and liver alcohol dehydrogenase (ADH) were observed. The administration of extracts from avocado and mustard seeds improved the state of oxidative stress and restored antioxidant enzyme activity. The expression levels of brain α-secretase and liver ADH genes were almost fully recovered at the molecular level.

Tài liệu tham khảo

Pagliaro LA, Pagliaro AM (2022) Drug and Substance Abuse Among Older Adults: Identification, Analysis, and Synthesis, 1st edn. Routledge, London. Doi: https://doi.org/10.4324/9781003010333 Stickel F, Datz C, Hampe J, Bataller R (2017) Pathophysiology and management of alcoholic liver disease: update 2016. Gut Liver 11:173 Yi S-W, Hong J-S, Yi J-J, Ohrr H (2016) Impact of alcohol consumption and body mass index on mortality from nonneoplastic liver diseases, upper aerodigestive tract cancers, and alcohol use disorders in Korean older middle-aged men: prospective cohort study. Medicine 95:39 Teschke R (2018) Alcoholic liver disease: alcohol metabolism, cascade of molecular mechanisms, cellular targets, and clinical aspects. Biomedicines 6:106 Hermoso DAM, Shimada LBC, Gilglioni EH, Constantin J, Mito MS, Hermoso APM, Salgueiro-Pagadigorria CL, Iwamoto ELI (2016) Melatonin protects female rats against steatosis and liver oxidative stress induced by oestrogen deficiency. Life Sci 157:178–186 Maithreyi R, Janani AV, Krishna R, Shweta A, Edwin RR, Mohan SK (2010) Erythrocyte lipid peroxidation and antioxidants in chronic alcoholics with alcoholic liver disease. Asian J Pharm Clin Res 3(3):183–185 Rahman S, Engleman EA, Bell RL (2016) Recent advances in nicotinic receptor signalling in alcohol abuse and alcoholism. Prog Mol Biol Transl Sci 137:183–201 Shield KD, Parry C, Rehm J (2013) Chronic diseases and conditions related to alcohol use. Alcohol Res 35:155 Casetta I, Govoni V, Granieri E (2005) Oxidative stress, antioxidants and neurodegenerative diseases. Curr Pharm Des 11:2033–2052 Laitinen MH, Ngandu T, Rovio S, Helkala E-L, Uusitalo U, Viitanen M, Nissinen A, Tuomilehto J, Soininen H, Kivipelto M (2006) Fat intake at midlife and risk of dementia and Alzheimer’s disease: a population-based study. Dement Geriatr Cogn Disord 22:99–107 Al-Asmari AK, Al-Elaiwi AM, Athar MT, Tariq M, Al Eid A, Al-Asmary SM (2014) A review of hepatoprotective plants used in saudi traditional medicine. Evid Based Complement Alternat Med 2014:890842 Wu J, Wang Y, Zhang Z, Yu B (2015) Herbal medicine in the treatment of Alzheimer’s disease. Chin J Integr Med 21:102–107 Chang Z, Wang Y-C, Tian D, Hu W-Y, Wang Z-Y, Liu G-L, Ma H-P, Hu Y-L, Wu B, Han Z-Y (2022) Medication rules in herbal medicine for mild cognitive impairment: a network pharmacology and data mining study. Evid Based Complement Alternat Med 2022:2478940 Majeed T, Bhat NA (2022) Health benefits of plant extracts. In: Shabir AM, Annamalai MK, Manzoor AS (eds) Plant extracts: applications in the food industry. Academic Press, London, pp 269–294 Verma A, Sharma A, Rai PK (2019) Impact of soxhlet extraction method on oil yield and antioxidant potential of Brassica juncea. J Pharmacogn Phytochem 8(4):1134–1137 Jimenez P, Garcia P, Quitral V, Vasquez K, Parra-Ruiz C, Reyes-Farias M, Garcia-Diaz DF, Robert P, Encina C, Soto-Covasich J (2021) Pulp, leaf, peel and seed of avocado fruit: a review of bioactive compounds and healthy benefits. Food Rev Intl 37:619–655 Parikh H, Pandita N, Khanna A (2015) Phytoextract of Indian mustard seeds acts by suppressing the generation of ROS against acetaminophen-induced hepatotoxicity in HepG2 cells. Pharm Biol 53:975–984 Buege JA, Aust SD (1978) [30] Microsomal lipid peroxidation. In: Methods in enzymology. Elsevier, Amsterdam, pp 302–310 Fossati P, Prencipe L, Berti G (1980) Use of 3, 5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clin Chem 26:227–231 Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169 Beutler E (1963) Improved method for the determination of blood glutathione. J lab clin Med 61:882–888 Habig W, Pabst M, Jakoby W (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249(22):7130–7139 Nishikimi M, Rao NA, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46:849–854 Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. https://doi.org/10.1016/s0076-6879(84)05016-3 Masalkar PD, Abhang SA (2005) Oxidative stress and antioxidant status in patients with alcoholic liver disease. Clin Chim Acta 355:61–65 Gupta SC, Hevia D, Patchva S, Park B, Koh W, Aggarwal BB (2012) Upsides and downsides of reactive oxygen species for cancer: the roles of reactive oxygen species in tumorigenesis, prevention, and therapy. Antioxid Redox Signal 16:1295–1322 Shati AA, Elsaid FG (2009) Effects of water extracts of thyme (Thymus vulgaris) and ginger (Zingiber officinale Roscoe) on alcohol abuse. Food Chem Toxicol 47:1945–1949 Chauhan NM, Mohan Karuppayil S (2021) Dual identities for various alcohols in two different yeasts. Mycology 12:25–38 Melis M, Carta G, Pistis M, Banni S (2013) Physiological role of peroxisome proliferator-activated receptors type α on dopamine systems. CNS Neurol Disord Drug Targets 12(1):70–77. https://doi.org/10.2174/1871527311312010012. (PMID: 23394525) Cannady R, Nimitvilai-Roberts S, Jennings SD, Woodward JJ, Mulholland PJ (2020) Distinct region-and time-dependent functional cortical adaptations in C57BL/6J mice after short and prolonged alcohol drinking. eNeuro. Doi: https://doi.org/10.1523/ENEURO.0077-20.2020 Rehm J, Gmel GE Sr, Gmel G, Hasan OSM, Imtiaz S, Popova S, Probst C, Roerecke M, Room R, Samokhvalov A (2017) The relationship between different dimensions of alcohol use and the burden of disease—an update. Addiction 112:968–1001 Hutchinson WL, Du M, Johnson PJ, Williams R (1991) Fucosyltransferases: differential plasma and tissue alterations in hepatocellular carcinoma and cirrhosis. Hepatology 13:683–688 AlSalloom AAM (2016) An update of biochemical markers of hepatocellular carcinoma. Int J Health Sci (Qassim) 10:121 Gonzalez FJ (2005) Role of cytochromes P450 in chemical toxicity and oxidative stress: studies with CYP2E1. Mutat Res 569(1–2):101–110. https://doi.org/10.1016/j.mrfmmm.2004.04.021. (PMID: 15603755) Srinivasan S, Guha M, Dong DW, Whelan KA, Ruthel G, Uchikado Y, Natsugoe S, Nakagawa H, Avadhani NG (2016) Disruption of cytochrome c oxidase function induces the Warburg effect and metabolic reprogramming. Oncogene 35:1585–1595 Bak MJ, Truong V-L, Ko S-Y, Nguyen XNG, Ingkasupart P, Jun M, Shin JY, Jeong W-S (2016) Antioxidant and hepatoprotective effects of procyanidins from wild grape (Vitis amurensis) seeds in ethanol-induced cells and rats. Int J Mol Sci 17:758 Silva-Adaya D, Garza-Lombó C, Gonsebatt ME (2021) Xenobiotic transport and metabolism in the human brain. Neurotoxicology 86:125–138 Agarwal DP, Goedde HW (2012) Alcohol metabolism, alcohol intolerance and alcoholism. Springer, Berlin Eriksson CJP (2001) The role of acetaldehyde in the actions of alcohol (update 2000). Alcohol Clin Exp Res 25:15S-32S Bagnardi V, Blangiardo M, La Vecchia C, Corrao G (2001) Alcohol consumption and the risk of cancer: a meta-analysis. Alcohol Res Health 25(4):263–270 Hou Y-C, Huang C-L, Lu C-L, Zheng C-M, Lin Y-F, Lu K-C, Chung Y-L, Chen R-M (2021) The role of plasma neurofilament light protein for assessing cognitive impairment in patients with end-stage renal disease. Front Aging Neurosci 13:657794. https://doi.org/10.3389/fnagi.2021.657794.PMID:34122041;PMCID:PMC8192845 de Strooper B, Vassar R, Golde T (2010) The secretases: enzymes with therapeutic potential in Alzheimer disease. Nat Rev Neurol 6:99–107 Sasaoka N, Sakamoto M, Kanemori S, Kan M, Tsukano C, Takemoto Y, Kakizuka A (2014) Long-term oral administration of hop flower extracts mitigates Alzheimer phenotypes in mice. PLoS ONE 9:e87185 Gonzalez-Lima F, Cada A (1998) Quantitative histochemistry of cytochrome oxidase activity. In: Gonzalez-Lima F (ed) cytochrome oxidase in neuronal metabolism and Alzheimer’s disease. Springer, Boston Anandatheerthavarada HK, Biswas G, Robin M-A, Avadhani NG (2003) Mitochondrial targeting and a novel transmembrane arrest of Alzheimer’s amyloid precursor protein impairs mitochondrial function in neuronal cells. J Cell Biol 161:41–54 Ding H, Han C, Guo D, Chin Y-W, Ding Y, Kinghorn AD, D’Ambrosio SM (2009) Selective induction of apoptosis of human oral cancer cell lines by avocado extracts via a ROS-mediated mechanism. Nutr Cancer 61:348–356 Ojewole JAO, Amabeoku GJ (2006) Anticonvulsant effect of Persea americana Mill (Lauraceae) (Avocado) leaf aqueous extract in mice. Phytother Res 8:696–700. https://doi.org/10.1002/ptr.1940 Larijani LV, Ghasemi M, AbedianKenari S, Naghshvar F (2014) Evaluating the effect of four extracts of avocado fruit on esophageal squamous carcinoma and colon adenocarcinoma cell lines in comparison with peripheral blood mononuclear cells. Acta Med Iran 52(3):201–205 Pahua-Ramos ME, Ortiz-Moreno A, Chamorro-Cevallos G, Hernández-Navarro MD, Garduño-Siciliano L, Necoechea-Mondragón H, Hernández-Ortega M (2012) Hypolipidemic effect of avocado (Persea americana Mill) seed in a hypercholesterolemic mouse model. Plant Foods Hum Nutr 67:10–16 Al-Dosari MS (2011) Hypolipidemic and antioxidant activities of avocado fruit pulp on high cholesterol fed diet in rats. Afr J Pharm Pharmacol 5(12):1475–1483 Sarwar MF, Sarwar MH, Sarwar M, Qadri NA, Moghal S (2013) The role of oilseeds nutrition in human health: a critical. J Cereals oilseeds 4(8):97–100 Singh S, Bhatia A, Tomer R, Kumar V, Singh B, Singh SD (2013) Synergistic action of tropospheric ozone and carbon dioxide on yield and nutritional quality of Indian mustard (Brassica juncea (L.) Czern.). Environ Monit Assess 185:6517–6529 Yuan H, Zhu M, Guo W, Jin L, Chen W, Brunk UT, Zhao M (2011) Mustard seeds (Sinapis Alba Linn) attenuate azoxymethane-induced colon carcinogenesis. Redox Rep 16:38–44 Geun Kim H, Sook OhM (2012) Herbal medicines for the prevention and treatment of Alzheimer’s disease. Curr Pharm Des 18(1):57–75 Ghibelli L, Coppola S, Fanelli C, Rotilio G, Civitareale P, Scovassi AI, Ciriolo MR (1999) Glutathione depletion causes cytochrome c release even in the absence of cell commitment to apoptosis. FASEB J 13:2031–2036 Sharma DR, Sunkaria A, Bal A, Bhutia YD, Vijayaraghavan R, Flora SJS, Gill KD (2009) Neurobehavioral impairments, generation of oxidative stress and release of pro-apoptotic factors after chronic exposure to sulphur mustard in mouse brain. Toxicol Appl Pharmacol 240:208–218 Dua A, Chander S, Agrawal S, Mahajan R (2014) Antioxidants from defatted Indian Mustard (Brassica Juncea) protect biomolecules against in vitro oxidation. Physiol Mol Biol Plants 20:539–543