Multiplex Genome Engineering Using CRISPR/Cas Systems

American Association for the Advancement of Science (AAAS) - Tập 339 Số 6121 - Trang 819-823 - 2013
Le Cong1,2, F. Ann Ran1,3, David Cox1,4, Shiquan Lin1,5, Robert P. J. Barretto6, Naomi Habib1, Patrick D. Hsu1,3, Xuebing Wu7, Wenyan Jiang8, Luciano A. Marraffini8, Feng Zhang1
1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA, and McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
2Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 02115, USA
3Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138 USA
4Harvard-MIT Health Sciences and Technology, Harvard Medical School, Boston, MA 02115, USA
5School of Life Sciences, Tsinghua University, Beijing 100084, China
6Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
7Computational and Systems Biology Graduate Program and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
8Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA

Tóm tắt

Genome Editing Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound to a CRISPR-associated endonuclease (see the Perspective by van der Oost ). Cong et al. (p. 819 , published online 3 January) and Mali et al. (p. 823 , published online 3 January) adapted this defense system to function as a genome editing tool in eukaryotic cells.

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