Effects of serotonin on electrical properties of Madin-Darby canine kidney cells
Tóm tắt
The present study has been performed to test for the influence of serotonin on the potential difference across the cell membrane (PD) of Madin-Darby canine kidney (MDCK)-cells. Under control conditions PD averages −48.6±0.6 mV (n=98). Increasing extracellular potassium concentration from 5.4 to 10 and 20 mmol/l depolarizes the cell membrane by +6.3±0.6 mV (n=6) and +14.1±1.0 mV (n=12), respectively. The cell membrane is transiently hyperpolarized to −67.8±0.8 mV (n=63) by 1 μmol/l serotonin. In the presence of serotonin, increasing extracellular potassium concentration from 5.4 to 20 mmol/l depolarizes the cell membrane by +26.4±1.0 mV (n=11). 1 mmol/l barium depolarizes the cell membrane by +15.7±1.3 mV (n=17) and abolishes the effect of step increases of extracellular potassium concentration from 5.4 to 10 mmol/l. In the presence of barium, serotonin leads to a transient hyperpolarization by −26.3±1.0 mV (n=16). During this transient hyperpolarization, the cell membrane is sensitive to extracellular potassium concentration despite the continued presence of barium. 10 μmol/l methysergide hyperpolarize the cell membrane by −7.2±2.0 mV (n=6). In the presence of 10μmol/l methysergide, the effect of serotonin is virtually abolished (+0.4±0.9 mV,n=6). 1 μmol/l ketanserin, a 5-HT2 receptor blocking agent, ICS 205-930, a 5-HT3 receptor blocking agent, and phentolamine, an unspecific α-receptor blocking agent, do not significantly modify the effect of serotonin. In the nominal absence of extracellular calcium, the effect of serotonin is markedly reduced. In conclusion, serotonin hyperpolarizes MDCK-cells by increasing apparent potassium conductance. This effect is transmitted by 5-HT1 receptors and depends on extracellular calcium.
Tài liệu tham khảo
Abboud HE, Dousa TP (1983) Renal metabolism and actions of histamine and serotonin. Mineral Electrolyte Metab 9:246–259
Adams WB, Levitan IB (1982) Intracellular injection of protein kinase inhibitor blocks the serotonin-induced increase in K+ conductance in Aplysia neuron R15. Proc Natl Acad Sci USA 79:3877–3880
Aiton JF, Brown CDA, Ogden P, Simmons NL (1982) K+ transport in “tight” epithelial monolayers of MDCK cells. J Membr Biol 65:99–109
Apperley E, Humphrey PPA, Levy GP (1976) Receptors for 5-hydroxytryptamine and noradrenaline in rabbit isolated ear artery and aorta. Br J Pharmacol 58:211–221
Arner M, Högestätt ED (1986) Contractile effects of noradrenaline and 5-hydroxytryptamine in human hand veins: a pharmacological receptor characterization. Acta Physiol Scand 128:209–217
Benson JA, Levitan IB (1983) Serotonin increases an anomalously rectifying K+ current in the Aplysia neuron R15. Proc Natl Acad Sci USA 80:3522–3525
Berridge MJ, Fain JN (1979) Inhibition of phosphatidylinostol synthesis and the inactivation of calcium entry after prolonged exposure of the blowfly salivary gland to 5-hydroxytryptamine. Biochem J 178:59–69
Berta P, Seguin J, Vidal N, Haiech J, Mathieu M-N, Chevillard C (1986) Influence of Ca2+ on 5-HT2 − and α1-induced arterial contraction and phosphoinositide metabolism. Eur J Pharmacol 132:253–257
Brown CDA, Simmons NL (1981) Catecholamine-stimulation of Cl-secretion in MDCK cell epithelium. Biochim Biophys Acta 649:427–435
Brown CDA, Simmons NL (1982) K+ transport in “tight” epithelial monolayers of MDCK-cells: Evidence for a calcium-activated K+ channel. Biochim. Biophys Acta 690:95–105
Bruns C, Marmé D (1987) Pertussis toxin inhibits the angiotensin II and serotonin-induced rise of free cytoplasmic calcium in cultured smooth muscle cells from rat aorta. FEBS Lett 212:40–44
Cereijido M, Ehrenfeld J, Meza I, Martinez-Palomo A (1980) Structural and functional membrane polarity in cultured monolayers of MDCK cells. J Membr Biol 52:147–159
Chan Y-L, Huang KC (1973) Renal excretion ofd-tryptophan, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid in rats. Am J Physiol 224:140–143
Donowitz M (1983) Ca2+ in the control of active intestinal Na and Cl transport: involvement in neurohumoral action. Am J Physiol 245:G165-G177
Donowitz M, Charney AN, Heffernan JM (1977) Effect of serotonin treatment on intestinal transport in the rabbit. Am J Physiol 232:E85-E94
Donowitz M, Asarkof N, Pike G (1980) Calcium dependence of serotonin-induced changes in rabbit ileal electrolyte transport. J Clin Invest 66:341–352
Donowitz M, Tai Y-H, Asarkof N (1980) Effect of serotonin on active electrolyte transport in rabbit ileum, gallbladder and colon. Am J Physiol 239:G463-G472
Fain JN, Berridge MJ (1979) Relationship between hormonal activation of phosphatidylinositol hydrolysis, fluid secretion and calcium flux in the blowfly salivary gland. Biochem J 178:45–58
Feniuk W (1984) An analysis of 5-hydroxytryptamine receptors mediating contraction of isolated smooth muscle. Neuropharmacology 23:1467–1472
Frattini P, Cucchi ML, Santagostino G, Corona GL (1979) A sensitive fluorimetric method for determination of platelet-bound and plasma free serotonin. Clin Chim Acta 92:353–360
Gaush CR, Hard WL, Smith TF (1966) Characterization of an established line of canine kidney cells. P.S.E.M.M. 122:931–935
Gstraunthaler G, Pfaller W, Kotanko P (1985) Biochemical characterization of renal epithelial cell cultures (LLC-PK1 and MDCK). Am J Physiol 248:F536-F544
Handler JS, Perkins FM, Johnson JP (1980) Studies of renal cell function using cell culture techniques. Am J Physiol 238:F1-F9
Kanaide H, Hasegawa M, Kobayashi S, Nakamura M (1987) Serotonin-induced cytosolic free calcium transients in cultured vascular smooth muscle cells. Biochem Biophys Res Commun 143:532–538
Kolb HA, Brown CDA, Murer H (1985) Identification of a voltage-dependent anion channel in the apical membrane of a Cl-secretory epithelium (MDCK). Pflügers Arch 403:262–265
Kolb H-A, Paulmichl M, Lang F (1987) Epinephrine activates outward rectifying K channel in Madin-Darby canine kidney cells. Pflügers Arch 408:584–591
Lang F, Defregger M, Paulmichl M (1986) Apparent chloride conductance of subconfluent Madin-Darby canine kidney cells. Pflügers Arch 407:158–162
Lemos JR, Levitan IB (1984) Intracellular injection of guanyl nucleotides alters the serotonin-induced increase in potassium conductance in Aplysia neuron R15. J Gen Physiol 83:269–285
Lever JE (1979) Regulation of dome formation in differentiated epithelial cell cultures. J Supramol Struct 12:259–272
Levitan IB (1985) Phosphorylation of ion channels. J Membr Biol 87:177–190
Lloyd P (1980) Modulation of neuromuscular activity of 5-hydroxytryptamine and endogenous peptides in the smail,Helix aspersa. J Comp Physiol 139:333–339
Madin SH, Darby NB (1958) As catalogued in: American Type Culture Collection. Catalogue Strains 2:574–576
McRoberts JA, Erlinger S, Rindler MJ, Saier Jr MH (1982) Furosemide-sensitive salt transport in the Madin-Darby canine kidney cell line. J Biol Chem 257:2260–2266
Misfeldt DS, Hamamoto ST, Pitelka DR (1976) Transepithelial transport in cell culture. Proc Natl Acad Sci USA 73:1212–1216
Park CS, Chu CS, Park YS, Hong SK (1968) Effect of 5-hydroxytryptamine on renal function of the anesthetized dog. Am J Physiol 214:384–388
Paulmichl M, Gstraunthaler G, Lang F (1985) Electrical properties of Madin-Darby canine kidney cells. Effects of extracellular potassium and bicarbonate. Pflügers Arch 405:102–107
Paulmichl M, Defregger M, Lang F (1986) Effects of epinephrine on electrical properties of Madin-Darby canine kidney cells. Pflügers Arch 406:367–371
Paulmichl M, Friedrich F, Lang F (1986) Electrical properties of Madin-Darby canine kidney cells. Effects of extracellular sodium and calcium. Pflügers Arch 407:258–263
Paulmichl M, Friedrich F, Lang F (1987) Effects of bradykinin on electrical properties of Madin-Darby canine kidney epithelioid cells. Pflügers Arch 408:408–413
Paupardin-Tritsch D, Hammond C, Gerschenfeld HM (1986) Serotonin and cyclic GMP both induce an increase of the calcium current on the same identified molluscan neurons. J Neurosci 6:2715–2723
Paupardin-Tritsch D, Hammond C, Gerschenfeld HM, Nairn AC, Greengard P (1986) cGMP-dependent protein kinase enhances Ca2+ current and potentiates the serotonin-induced Ca2+ current increase in snail neurons. Nature 323:812–814
Pellmar TC, Carpenter DO (1979) Voltage-dependent calcium current induced by serotonin. Nature 277:483–484
Pellmar TC (1981) Does cyclic 3′,5′-adenosine monophosphate act as second messenger in a voltage-dependent response to 5-hydroxytryptamine in Aplysia. Br J Pharmacol 74:447–756
Pellmar TC, Carpenter DO (1980) Serotonin induces a voltage-sensitive calcium current in neurons ofAplysia californica. J Neurophysiol 44:423–439
Prince WT, Berridge MJ, Rasmussen H (1972) Role of calcium and adenosine-3′: 5′-cyclic monophosphate in controlling fly salivary gland secretion. Proc Natl Acad Sci USA 69:553–557
Putney Jr JW (1979) Stimulus-permeability coupling: Role of calcium in the receptor regulation of membrane permeability. Pharmacol Rev 30:209–245
Ram JL, Gole D, Shukla U, Greenberg L (1983) Serotonin-activated adenylate cyclase and the possible role of cyclic AMP in modulation of buccal muscle concentration in Aplysia. J Neurobiol 14:113–121
Richardson BP, Engel G (1986) The pharmacology and function of 5-HT3 receptors. TINS(Sept): 424–428
Richardson BP, Engel G, Donatsch P, Stadler PA (1985) Identification of serotonin M-receptor subtypes and their specific blockade by a new class of drugs. Nature 316:126–131
Richardson JCW, Scalera V, Simmons NL (1981) Identification of two strains of MDCK cells which resemble separate nephron tubule segments. Biochim Biophys Acta 673:26–36
Rindler MJ, Saier Jr MH (1981) Evidence for Na+/H+ antiport in cultured dog kidney cells (MDCK). J Biol Chem 256:10820–10825
Rindler MJ, Chuman LM, Shaffer L, Saier Jr MH (1979) Retention of differentiated properties in an established dog kidney: Epithelial cell line (MDCK). J Cell Biol 81:635–648
Rindler MJ, Taub M, Saier Jr MH (1979) Uptake of22Na+ by cultured dog kidney cells (MDCK). J Biol Chem 254:11431–11439
Saier Jr MH, Boyden DA (1984) Mechanism, regulation and physiological significance of the loop diuretic-sensitive NaCl−KCl symport system in animal cells. Mol Cell Biochem 59:11–32
Schultz SG (1980) Basic principles of membrane transport. Cambridge University Press, London
Shah SV, Northrup TE, Hui YSF, Dousa TP (1979) Action of serotonin (5-hydroxytryptamine) on cyclic nucleotides in glomeruli of rat renal cortex. Kidney Int 15:463–472
Simmons NL (1981) Stimulation of Cl-secretion by exogenous ATP in cultured MDCK epithelial monolayers. Biochim Biophys Acta 646:231–242
Simmons NL (1981) The action of ouabain upon chloride secretion in cultured MDCK epithelium. Biochim Biophys Acta 646:243–250
Simmons NL (1982) Cultured monolayers of MDCK cells: A novel model system for the study of epithelial development and function. Gen Pharmacol 13:287–291
Sole MJ, Madapallimattam A, Baines AD (1986) An active pathway for serotonin synthesis by renal proximal tubules. Kidney Int 29:689–694
Stier Jr CT, Itskovitz HD (1985) Formation of serotonin by rat kidneys in vivo. Proc R Soc Exp Biol Med 180:550–557
Stier Jr CT, McKendall G, Itskovitz HD (1984) Serotonin formation in nonblood-perfused rat kidneys. J Pharmacol Exp Ther 228:53–56
Stier Jr CT, Brewer TF, Dick LB, Wynn N, Itskovitz HD (1986) Formation of biogenic amines by isolated kidneys of spontaneously hypertensive rats. Life Sci 38:7–14
Taub M, Chuman L, Saier Jr MH, Sato G (1979) Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone supplemented, serum-free medium. Proc Natl Acad Sci USA 76:3338–3342
Valentich JD (1981) Morphological similarities between the dog kidney cell line MDCK and the mammalian cortical collecting tubule. Ann NY Acad Sci 372:384–405
Valentich JD, Tchao R, Leighton J (1979) Hemicyst formation stimulated by cyclic AMP in dog kidney cell line MDCK. J Cell Physiol 100:291–304
Weiss KR, Mandelbaum DE, Schonberg M, Kupfermann I (1979) Modulation of buccal muscle contractility by serotonergic metacerebral cells in Aplysia: Evidence for a role of cyclic adenosine monophosphate. J Neurophysiol 42:791–803
Williamson JR, Cooper RH, Joseph SK, Thomas AP (1985) Inositol trisphosphate and diacylglycerol as intracellular second messengers in liver. Am J Physiol 248:C203-C216