Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague–Dawley rats
Tóm tắt
The present study evaluated the effects of asiatic acid (AA), a pentacyclic triterpenoid from Centella asiatica on lipid metabolism parameters in a rat model of obesity induced using a high fat diet (HFD) for 42 days. AA (20 mg/kg body weight [BW]) was administered orally once daily for 42 days, and an orlistat-treated group of rats (10 mg/kg BW) was included for comparison. Changes in BW, blood glucose levels, insulin resistance and leptin, adiponectin, amylase, and lipase levels in the blood; lipid profiles of plasma; liver antioxidants levels; and acetyl CoA carboxylase(ACC), uncoupling protein-2 (UCP2), and carnitine palmitoyltransferase-1 (CPT1) mRNA expression were observed in the experimental rats. Our results revealed that AA (20 mg/kg BW), similar to orlistat, reduced the increase in BW; increased bone mineral contents and bone mineral densities; reduced blood glucose levels, insulin resistance, leptin, plasma lipid levels; increased adiponectin, amylase, lipase levels in the blood; showed antioxidant activity; and altered mRNA expression of lipid metabolism-related genes, including ACC, UCP 2, and CPT 1, in the HFD-fed rats. From these results, we concluded that AA possesses significant anti-obesity potential through the suppression of BW gain, lipid lowering action, development of insulin and leptin sensitivity, antioxidant activity, and increased mRNA expression of lipid metabolism-related genes.
Tài liệu tham khảo
Despres JP (2012) Body fat distribution and risk of cardiovascular disease: an update. Circulation 126:1301–1313
Saravanan G, Ponmurugan P, Deepa MA, Senthilkumar B (2014) Anti-obesity action of gingerol: effect on lipid profile, insulin, leptin, amylase and lipase in male obese rats induced by a high-fat diet. J Sci Food Agric 94:2972–2977
Ferranti S, Mozaffarian D (2008) The perfect storm: obesity, adipocyte dysfunction, and metabolic consequences. Clin Chem 54:6945–6955
Jindal A, Brietzke S, Sowers JR (2012) Obesity and the cardiorenal metabolic syndrome: therapeutic modalities and their efficacy in improving cardiovascular and renal risk factors. Car Ren Med 2(4):314–327
George M, Rajaram M, Shanmugam E (2014) New and emerging drug molecules against obesity. J Cardiovasc Pharmacol Ther 19(1):65–76
Colagiuri S (2010) Diabesity: therapeutic options. Diabetes Obes Metab 12:463–473
Kishino E, Ito T, Fujita K, Kiuchi Y (2006) A mixture of the Salacia reticulata (Kotala himbutu) aqueous extract and cyclodextrin reduces the accumulation of visceral fat mass in mice and rats with high-fat diet-induced obesity. J Nutr 136:433–439
Zhao Y, Shu P, Zhang Y, Lin L (2014) Effect of Centella asiatica on oxidative stress and lipid metabolism in hyperlipidemic animal models. Oxid Med Cell Longev 154:1–7
Huang SS, Chiu CS, Chen HJ, Hou WC, Sheu MJ, Lin YC, Shie PH, Huang GJ (2011) Antinociceptive activities and the mechanisms of anti-inflammation of asiatic acid in mice. Evid Based Complement Altern Med. doi:10.1155/2011/895857
Ramachandran V, Saravanan R, Senthilraja P (2014) Antidiabetic and antihyperlipidemic activity of asiatic acid in diabetic rats, role of HMG CoA: in vivo and in silico approaches. Phytomed 212:225–232
Ramachandran V, Saravanan R (2013) Asiatic acid prevents lipid peroxidation and improves antioxidant status in rats with streptozotocin-induced diabetes. J Funct Foods 5:1077–1087
Ji W, Zhao M, Wang M, Yan W, Liu Y, Ren S (2017) Effects of canagliflozin on weight loss in high-fat diet-induced obese mice. PLoS ONE 12(6): e0179960.
Buettner R, Schölmerich J, Bollheimer LC (2007) High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity 15:798–808
Naidu PB, Uddandrao VS, Naik RR, Suresh P, Meriga B, Begum MS, Pandiyan R, Saravanan G (2016) Ameliorative potential of gingerol: promising modulation of inflammatory factors and lipid marker enzymes expressions in HFD induced obesity in rats. Mol Cell Endocrinol 419:139–147
Oben J, Kuate D, Agbor G, Momo C, Talla X (2006) The use of a Cissus quadrangularis formulation in the management of weight loss and metabolic syndrome. Lip Heal Dis 5:24
Srinivasan K, Patole PS, Kaul CL, Ramarao P (2004) Reversal of glucose intolerance by pioglitazone in high-fat diet fed rats. Exp Clin Pharmacol 26:327–333
Naidu PB, Ponmurugan P, Begum MS, Mohan K, Meriga B, Naik RR, Saravanan G (2015) Diosgenin reorganises hyperglycaemia and distorted tissue lipid profile in high-fat diet–streptozotocin-induced diabetic rats. J Sci Food Agric 95:3177–3182
Randle PJ, Garland PB, Hales CN, Newsholme EA (1963) The glucose fatty-acid cycle. Its role in insulin sensitivity andmetabolic disturbances in diabetes mellitus. Lancet 1:785–789
Saravanan G, Ponmurugan P (2012) Ameliorative potential of S-allylcysteine: effect on lipid profile and changes in tissue fattyacid composition in experimental diabetes. Exp Toxicol Pathol 64:639–644
Fungwe TV, Cagen LM, Cook GA (1993) Dietary cholesterol stimulated hepatic biosynthesis of triglyceride and reduces oxidation of fatty acids in the rat. J Lipid Res 34:933–941
Kim Y, Park T (2008) Genes are differentially expressed in the epididymal fat of rats rendered obese by a high-fat diet. Nutr Res 28:414–422
Zhou CJ, SongHuang JQ (2013) Sweet tea leaves extract improves leptin resistance in diet-induced obese rats. J Ethnopharm 145:386–392
Manuel TV, Sam JB (2007) Role of dietary so y protein in obesity. Int J Med Sci 4:72–82
Liu J, He T, Lu Q, Shang J, Sun H, Zhang L (2010) Asiatic acid preserves β cell mass and mitigates hyperglycemia in streptozocin-induced diabetic rats. Diabetes Metab Res Rev 26:448–454
Garg A (2006) Adipose tissue dysfunction in obesity and lipodystrophy. Clin Cornerstone 8:7–13
Cnop M, Landchild MJ, Vidal J, Havel PJ, Knowles NG, Carr DR (2002) The concurrent accumulation of intra-abdominal and subcutaneous fat explains the association between insulin resistance and plasma leptin concentrations. Diabetes 51:1005–1015
Arita Y, Kihara S, Ouchi N (1999) Paradoxical decrease of an adipose-specific protein, adiponectin in obesity. Biochem Biophys Res Commun 257:79–83
Kamohara S, Burcelin R, Halaas JL, Friedman JM, Charron MJ (1997) Acute stimulation of glucose metabolism in mice by leptin treatment. Nature 389:374–377
Staiger H, Haring HU (2005) Adipocytokines: fat-derived humoral mediators of metabolic homeostasis. Exp Clin Endocrinol Diabetes 113:67–79
Crozier A, Jaganath IB, Clifford MN (2009) Dietary phenolics: chemistry, bioavailability and effects on health. Nat Prod Rep 26:1001–1043
Ono Y, Hattori E, Fukaya Y, Imai S, Ohizumi Y (2006) Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J Ethnopharm 106:238–244
Nakajima K, Muneyuki T, Munakata H (2011) Revisiting the cardiometabolic relevance of serum amylase. BMC Res Not 4:419
Tundis R, Loizzo MR, Menichini F (2010) Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini Rev Med Chem 10:315–331
Layer P, Rizza RA, Zinsmeister AR, Carlson GL, DiMagno EP (1986) Effect of a purified amylase inhibitor on carbohydrate tolerance in normal subjects and patients with diabetes mellitus. Mayo Clin Proce 61:442–447
Naidu PN, Uddandrao VS, Sasikumar V, Naik RR, Pothani S, Begum MS, Varatharaju C, Meriga B, Kalaivani A, Saravanan G (2017) Reversal of endothelial dysfunction in aorta of streptozotocinnicotinamide-induced type-2 diabetic rats by S-Allylcysteine. Mol Cell Biochem. doi:10.1007/s11010-017-2994-0
Jin D, Xua Y, Mei X (2013) Antiobesity and lipid lowering effects of theaflavins on high-fat diet induced obese rats. J Funct Foods 5:1142–1150
Park Y, Storkson JM, Liu W, Albright J, Cook ME, Pariza MW (2004) Structure–activity relationship of conjugated linoleic acid and its cognates in inhibiting heparin-releasable lipoprotein lipase and glycerol release from fully differentiated 3T3-L1 adipocytes. J Nutr Biochem 15:561–569
Torre-Villalvazo I, Tovar AR, Ramos-Barragan VE, Cerbon-Cervantes MA, Torres N (2008) Soy protein ameliorates metabolic abnormalities in liver and adipose tissue of rats fed a high fat diet. J Nutr 138:462–468
Garjani A, Fathiazad F, Zakheri A, Akbari NA, Azarmie Y (2009) The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status, and vascular function in hypercholesterolemic rats. J Ethnopharm 126:525–532
Yongqi G, Guanzhong W, Xin S, Hongxia Y, Juan Z (2009) Antiobesity action of a daidzein derivative on male obese mice induced by a high-fat diet. Nutr Res 29:656–663
Moller DE (2001) New drug targets for type 2 diabetes and the metabolic syndrome: a review. Nature 414:821–827
Uddandrao VS, Brahmanaidu P, Saravanan G (2016) Therapeutical perspectives of S-allylcysteine: effect on diabetes and other disorders in animal models. Car Hem Age Med Chem. doi:10.2174/1871525714666160418114120
Winterbourn CC (1995) Concerted antioxidant activity of glutathione and superoxide dismutase. In: Packer L, Fuchs J (eds) Biothiols in health and disease. Marcel Dekker Inc, New York, pp 117–134
Saravanan G, Ponmurugan P (2011) Ameliorative potential of S-allylcysteine on oxidative stress in STZ induced diabetic rats. Chem Biol Interact 189:100–106
Uddandrao VS, Brahmanaidu P, Meriga B, Saravanan G (2016) The potential role of S-allylcysteine as antioxidant against various disorders in animal models. Oxid Antioxid Med Sci 5(3):79–86
Naidu PB, Harishankar N, Meriga B, Pothana S, Sajjalaguddam RR (2015) Effects of Piper nigrum extracts: Restorative perspectives of high-fat diet-induced changes on lipid profile, body composition, and hormones in Sprague-Dawley rats. Pharm Biol 53(9):1318–1328
Naidu PB, Uddandrao VS, Pothani S, Naik RR, Begum MS, Varatharaju C, Pandiyan R, Saravanan G (2016) Effects of S-allylcysteine on biomarkers of polyol pathway in experimental type II diabetes in rats. Can J Diabetes 40:442–448
Van Herpen NA, Schrauwen-Hinderling VB (2008) Lipid accumulation in non-adipose tissue and lipotoxicity. Physiol Behav 94:231–241
Harbilas D, Braulta A, Valleranda D (2012) Populus balsamifera L. (Salicaceae) mitigates the development of obesity and improves insulin sensitivity in a diet-induced obese mouse model. J Ethnopharm 141:1012–1020
Wanga LJ, Zhang HW, Zhoua JY (2014) Betaine attenuates hepatic steatosis by reducing methylation of the MTTP promoter and elevating genomic methylation in mice fed a high-fat diet. J Nutr Biochem 25:329–336
Andrews ZB, Erion DM, Beiler R, Choi CS, Shulman GI, Horvath TL (2010) Uncoupling protein-2 decreases the lipogenic actions of ghrelin. Endocrinology 151:2078–2086
Ferre P, Foufelle F (2010) Hepatic steatosis: a role for de novo lipogenesis and the transcription factor SREBP-1c. Diabetes Obes Metab 12:83–92