Bovine pulmonary surfactant: Chemical composition and physical properties
Tóm tắt
Bovine pulmonary surfactant was obtained by endotracheal lavage of lungs from newly slaughtered cows followed by differential centrifugation. Lipid extracts of bovine surfactant contained 3% neutral lipid, mainly as cholesterol and diacylglycerol and 97% phospholipid. Phosphatidylcholine (79%) and phosphatidylglycerol (11%) accounted for most of the phospholipids with smaller amounts of phosphatidyl-ethanolamine, phosphatidylinositol, lyso-bis-phosphatidic, acid and sphingomyelin. Fatty acid analysis revealed high levels of palmitate in phosphatidylcholine and to a lesser extent phosphatidylglycerol, but not in the other diacylphospholipids. Phosphatidylcholine was 53% disaturated and phosphatidylglycerol was 23% disaturated. Monoenoic species accounted for the major proportion of the remaining, lipid. The protein content was 10% as estimated by the Lowry procedure and 5% when determined by amino acid analysis. Extraction with chloroform/methanol removed ca. 90% of the protein but had no effect on the surfactant properties as evaluated by a pulsating bubble technique.
Tài liệu tham khảo
Goerke, J. (1974) Biochim. Biophys. Acta 344, 241–261.
King, R.J. (1974) Fed. Proc. 33, 2238–2247.
Possmayer, F. (1982) in Biochemical Development of the Fetus and Neonate (Jones, C.T., ed.) pp. 337–391, Elsevier Biomedical Press, Amsterdam.
Farrell, P.M., and Avery, M.E. (1975) Am. Rev. Resp. Dis. 111, 657–688.
Perelman, R.H., Engle, M.J., and Farrell, P.M. (1981) Lung 159, 53–80.
Robertson, B. (1980) Lung 158, 57–68.
Robertson, B. (1981) Rev. Perinat. Med. 4, 337–379.
Fujiwara, T., Chida, S., Watabe, Y., Maeta, H., Morita, T., and Abe, T. (1980) Lancet 1, 55–59.
Smyth, J.A., Metcalfe, I.L., Duffy, P., Enhorning, G.E., Possmayer, F., Olley, P.M., and Bryan, M.H. (1981) Pediatr. Res. 15, 681.
Smyth, J.A., Metcalfe, I.L., Duffy, P., Enhorning, G.E., Possmayer, F., Olley, P.M., and Bryan, M.A. (1981) Clin. Invest. Med. 4, 43B.
Brain, J.D., and Frank, N.R. (1968) J. Appl. Physiol. 25, 63–69.
Bligh, E.G., and Dyer, W.J. (1959) Can. J. Biochem. Physiol. 37, 911–917.
Smith, N.B. (1982) Lipids 17, 464–468.
Touchstone, J.C., Chen, J.C., and Beaver, K.M. (1980) Lipids 15, 61–62.
Rouser, G., Fleischer, S., and Yamamoto, A. (1969) Lipids 5, 494–496.
Possmayer, F., Duwe, G., Hahn, M., and Buchnea, D. (1977) Can. J. Biochem. 55, 609–617.
Kates, M. (1972) in Techniques of Lipidology, pp. 568–569, North Holland/Elsevier, Amsterdam.
van Golde, L.M.G., and van Deenen, L.L.M. (1966) Biochim. Biophys. Acta 125, 496–509.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L., and Randall, R.J. (1951) J Biol. Chem. 193,265–275.
Metcalfe I.L., Enhorning, G.E., and Possmayer, F. (1980) J. Appl. Physiol. 49, 34–41.
Enhorning, G.E. (1977) J. Appl. Physiol. 43, 198–203.
Enhorning, G., Hill, D., Sherwood, G., Curtz, E., Robertson, B., and Bryan C. (1978) Am. J. Obstet. Gynecol. 132, 529–536.
Jobe, A., Ikegami, M., Glatz, T., Yoshida, Y., Diakomanolis, E., and Padbury, J. (1981) J. Clin. Invest. 67, 370–375.
Sanders, R.L. (1982) in Lung Development: Biological and Clinical Perspectives (Parrell, P.M., ed.) Vol. 1, pp. 193–210, Academic Press, New York.
King, R.J., and Clements, J.A. (1972) Am. J. Physiol. 223, 715–726.
Harwood, J.L., Desai, R., Hext, P., Tetley, T. and Richards, R. (1975) Biochim. J. 151, 707–714.
Rooney, S.A., Canavan, P.M., and Motoyame, E.K. (1974) Biochim. Biophys. Acta 360, 56–57.
Hallman, M., and Gluck, L. (1976) J. Lipid Res. 17, 257–263.
Suzuki, Y., Nakai, E., and Ohkawa, K. (1982) J. Lipid Res. 23, 53–61.
Ohno, K., Akino, T., and Fujiwara, T. (1978) Rev. Perinat. Med. 2, 227–280.
Sanders, R.L., and Longmore, W.J. (1975) Biochemistry 14, 835–840.
Hass, M.A., and Longmore, W.J. (1979) Biochim. Biophys. Acta 573, 166–174.
Hallman, M., Teramo, K., Kankaanpaa, K., Kulovich, M.V., and Gluck, L. (1980) Anna. Clin. Res. 12, 36–44.
Bangham, A.D., Morley, C.J., and Phillips, M.C. (1979) Biochim. Biophys. Acta 573, 552–556.
Gil, J., and Reiss, O.K. (1973) J. Cell. Biol. 58, 152–171.
Oulton, M., Martin, T.R., Faulkner, G.T., Stinson, D., and Johnson, J.P. (1980) Pediatr. Res. 14, 722–728.
Post, M., Batenburg, J.J., Schuurmans, E.A.J.M, Lavos, C.D., and van Golde, L.M.G. (1982) Exp. Lung Res. 3, 17–28.
King, R.J. (1977) Am. Rev. Res. Dis. 115, 735–795.
King, R.J., Martin, H., Mitts, D., and Holmstrom, F.M. (1977) J. Appl. Physiol. 43, 483–491.
Sueishi, K., and Benson, B.J. (1981) Biochim. Biophys. Acta 665, 442–453.
Hildebran, J.E., Georke, J., and Clements, J.A. (1979) J. Appl. Physiol. 47, 604–611.
Henderson, R.F., and Pfleger, R.C. (1972) Lipids 7, 492–494.
King, R.J., and MacBeth, M.C. (1979) Biochim. Biophys. Acta 557, 86–101.
Metcalfe, I.L., Burgoyne, R., and Enhorning, G. (1982) Pediatr. Res. 16, 834–839.