Platelet-derived growth factor and transforming growth factor-beta 1 differentially affect the synthesis of biglycan and decorin by monkey arterial smooth muscle cells.

Ovid Technologies (Wolters Kluwer Health) - Tập 13 Số 7 - Trang 1026-1036 - 1993
Elke Schönherr1, Hannu Järveläinen1, Michael G. Kinsella2, Linda J. Sandell3, Thomas N. Wight4
1Department of Pathology, School of Medicine, University of Washington, Seattle 98195.
2M G Kinsella Department of Pathology, School of Medicine, University of Washington, Seattle 98195.
3L J Sandell Department of Pathology, School of Medicine, University of Washington, Seattle 98195.
4T N Wight Department of Pathology, School of Medicine, University of Washington, Seattle 98195.

Tóm tắt

Platelet-derived growth factor (PDGF) and transforming growth factor-beta 1 (TGF-beta 1), two growth-regulatory peptides with opposite effects on arterial smooth muscle cell (ASMC) proliferation, were examined for their influence on the synthesis of two small chondroitin sulfate/dermatan sulfate proteoglycans (CS/DS PGs) called biglycan and decorin. Quiescent ASMCs treated with either PDGF or TGF-beta 1 for 24 hours increased [35S]sulfate incorporation into biglycan 3.3- and 2.9-fold, respectively, whereas the incorporation of [35S]sulfate into decorin was not significantly affected. Treatment with TGF-beta 1 but not PDGF more than doubled the steady-state level of messenger RNA (mRNA) transcripts hybridizing to a complementary DNA (cDNA) encoding biglycan. Both growth factors had little or no effect on steady-state levels of mRNA transcripts hybridizing to a decorin cDNA. Incorporation of [35S]sulfate into biglycan glycosaminoglycan (GAG) was maximal by 12 to 18 hours after either PDGF or TGF-beta 1 addition. Both PDGF and TGF-beta 1 increased the molecular sizes of biglycan and decorin. This increase was a result of the synthesis of longer GAG chains substituted on the core proteins of both PGs. PDGF but not TGF-beta 1 led to an increase of more than twofold in the ratio of 6'- to 4'-sulfated disaccharides in these newly synthesized GAG chains. These results indicate that PDGF and TGF-beta 1 have specific but different effects on the synthesis of small CS/DS PGs by monkey ASMCs in culture.

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Ovid Technologies (Wolters Kluwer Health)

Tập 13 Số 7

1026-1036

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