Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1

Molecular Microbiology - Tập 4 Số 6 - Trang 895-904 - 1990
Dominique Galli1, Friedrich Lottspeich2, Reinhard Wirth3
1Lehrstuhl für Mikrobiologie der Universität München, FRG.
2Max‐Planck‐Institut für Biochemie/Genzentrum Am Klopferspitz 80, 8033 Martinsried, FRG.
3Lehrstuhl für Mikrobiologie der Universität München, Maria‐Ward‐Straβe 1a, 8000 München 19, PRG.

Tóm tắt

Summary

The location of the structural gene for aggregation substance on the sex pheromone plasmid pAD1 of Enterococcus faecalis was determined using an oligonucleotide deduced from the N‐terminal amino acid sequence of the purified protein. The nucleotide sequence was determined for the corresponding region and two open reading frames (ORFs) could be identified. ORF1 codes for a small (Mr 13160) acidic protein of unknown function. The gene for aggregation substance (named asa1 was found to code for a protein of 1296 amino acids (Mr 142248). The protein has a signal peptide of 43 amino acids (the resulting (Mr for mature aggregation substance is 137429) and contains in its C‐terminal region a proline‐rich sequence, previously characterized as being involved in cell wall association, which is followed by a membrane anchor. The membrane anchor showed significant similarity to that of other Gram‐positive organisms, but no other similarities to surface proteins from Gram‐positive bacteria were found. In particular, no repeats on the DNA or protein level could be detected for pAD1‐specific aggregation substance. The protein contains the amino acid motifs Arg‐Gly‐Asp‐Ser and Arg‐Gly‐Asp‐Val (once each), which, it is proposed, play a crucial role in adherence to eukaryotic cells.

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Tài liệu tham khảo

Cleveland D.W., 1977, Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by electrophoresis, J Biol Chem, 252, 1102, 10.1016/S0021-9258(19)75212-0

Clewell D.B., 1983, Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917, J Bacteriol, 152, 1220, 10.1128/jb.152.3.1220-1230.1982

10.1128/JB.162.3.1212-1220.1985

10.1016/0147-619X(89)90041-3

10.1073/pnas.75.7.3479

10.1016/0147-619X(79)90029-5

10.1073/pnas.82.24.8582

10.1111/j.1432-1033.1988.tb14308.x

10.1128/JB.169.8.3473-3481.1987

10.1128/jb.168.1.6-12.1986

10.1128/jb.167.3.870-880.1986

10.1111/j.1365-2958.1989.tb00193.x

10.1007/BF00454863

10.1093/nar/15.5.2343

Hollingshead S.K., 1986, Complete nucleotide sequence of type 6M protein of the group A Streptococcus— repetitive structure and membrane anchor, J Biol Chem, 261, 1677, 10.1016/S0021-9258(17)35993-8

10.1007/BF00331578

Ike Y., 1984, Genetic analysis of the pAD1 pheromone response in Streptococcus faecalis. using transposon Tn917 as an insertional mutagen, J Bacteriol, 158, 777, 10.1128/jb.158.3.777-783.1984

10.1128/jb.172.1.155-163.1990

Kozak M., 1983, Comparison of Initiation of protein synthesis in prokaryotes, eukaryotes, and organelles, Microbiol Rev, 47, 1, 10.1128/mr.47.1.1-45.1983

10.1111/j.1475-4754.1982.tb01001.x

10.1128/jb.170.6.2618-2624.1988

10.1073/pnas.78.8.4689

10.1146/annurev.bi.55.070186.002011

10.1146/annurev.bi.57.070188.002111

10.1016/0378-1119(87)90383-0

Uhlen M., 1984, Complete sequence of the staphylococcal gene encoding protein A — a gene evolved through multiple duplications, J Biol Chem, 259, 1695, 10.1016/S0021-9258(17)43463-6

10.1007/BF00454864

10.1128/jb.165.3.831-836.1986

10.1099/00221287-129-4-1207

10.1016/0378-1119(85)90120-9

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Molecular Microbiology

Tập 4 Số 6

895-904

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