Effect of bone morphogenetic proteins‐4, '5 and '6 on DNA synthesis and expression of bone‐related proteins in cultured human periodontal ligament cells

Cell Biology International - Tập 28 - Trang 675-682 - 2004
Wan-Peng Xu1, Hideki Shiba1, Noriyoshi Mizuno1, Yuushi Uchida1, Yoshihiro Mouri1, Hiroyuki Kawaguchi1, Hidemi Kurihara1
1Department of Periodontal Medicine, Division of Frontier Medical Science, Programs for Biomedical Research, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8553, Japan

Tóm tắt

AbstractBone morphogenetic proteins (BMPs) have multiple functions in the development and growth of skeletal and extraskeletal tissues. Therefore, BMPs may regulate the regeneration of periodontal tissue. To investigate this issue, we examined the effects of BMP‐4, −5 and −6 on DNA synthesis and the expression of bone‐related proteins in cultures of human periodontal ligament (HPL) cells. The expression of bone‐related proteins was determined by Real‐time polymerase chain reaction and enzyme linked immunosorbent assay in cultures of HPL cells. DNA synthesis was estimated by measuring bromoderoxyuridine incorporation. It was found that BMP‐4, −5 and −6 enhanced DNA synthesis dose‐dependently. BMP‐4 and −5 increased the levels of osteopontin, BMP‐2, alkaline phosphatase and core binding factor alpha 1 mRNAs. BMP‐6 stimulated the expression of osteopontin, BMP‐2, ALPase and osteoprotegerin. These findings show that BMP‐4, −5 and −6 have different actions on the expression of bone‐related proteins and may play a role in the regeneration of periodontal tissue by promoting cell proliferation and protein expression.

Tài liệu tham khảo

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Cell Biology International

Tập 28

675-682

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