Use of mutant-assisted gene identification and characterization (MAGIC) to identify novel genetic loci that modify the maize hypersensitive response

Theoretical and Applied Genetics - Tập 123 - Trang 985-997 - 2011
Vijay Chaikam1, Adisu Negeri2, Rahul Dhawan2,3, Bala Puchaka4, Jiabing Ji4, Satya Chintamanani4,5, Emma W. Gachomo4, Allen Zillmer6, Timothy Doran6, Cliff Weil1, Peter Balint-Kurti7, Guri Johal4
1Department of Agronomy, Purdue University, West Lafayette, USA
2Department of Plant Pathology, NC State University, Raleigh, USA
3Monsanto Company, St. Louis, USA
4Botany and Plant Pathology, Purdue University, West Lafayette, USA
5Syngenta Seeds, Inc., Slater, USA
6Genomics Core Laboratory, David H. Murdock Research Institute (DHMRI), Kannapolis, USA
7Peter Balint-Kurti USDA-ARS Plant Science Research Unit, Department of Plant Pathology, North Carolina State University, Raleigh, USA

Tóm tắt

The partially dominant, autoactive maize disease resistance gene Rp1-D21 causes hypersensitive response (HR) lesions to form spontaneously on leaves and stems in the absence of pathogen recognition. The maize nested association mapping (NAM) population consists of 25 200-line subpopulations each derived from a cross between the maize line B73 and one of 25 diverse inbred lines. By crossing a line carrying the Rp1-D21 gene with lines from three of these subpopulations and assessing the F1 progeny, we were able to map several novel loci that modify the maize HR, using both single-population quantitative trait locus (QTL) and joint analysis of all three populations. Joint analysis detected QTL in greater number and with greater confidence and precision than did single population analysis. In particular, QTL were detected in bins 1.02, 4.04, 9.03, and 10.03. We have previously termed this technique, in which a mutant phenotype is used as a “reporter” for a trait of interest, Mutant-Assisted Gene Identification and Characterization (MAGIC).

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