Reprogramming the Cell Cycle for Endoreduplication in Rodent Trophoblast Cells

Molecular Biology of the Cell - Tập 9 Số 4 - Trang 795-807 - 1998
A MacAuley1, James C. Cross2, Zena Werb3
1Department of Anatomy, University of California, San Francisco, California 94143-0750, USA.
2Samuel Lunenfeld Research Institute Mount Sinai Hospital and Departments of Obstetrics and Gynecology, and Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5G 1X5, Canada
3#N#*Department of Anatomy, University of California, San Francisco, California 94143-0750; and

Tóm tắt

Differentiation of trophoblast giant cells in the rodent placenta is accompanied by exit from the mitotic cell cycle and onset of endoreduplication. Commitment to giant cell differentiation is under developmental control, involving down-regulation of Id1and Id2, concomitant with up-regulation of the basic helix-loop-helix factor Hxt and acquisition of increased adhesiveness. Endoreduplication disrupts the alternation of DNA synthesis and mitosis that maintains euploid DNA content during proliferation. To determine how the mammalian endocycle is regulated, we examined the expression of the cyclins and cyclin-dependent kinases during the transition from replication to endoreduplication in the Rcho-1 rat choriocarcinoma cell line. We cultured these cells under conditions that gave relatively synchronous endoreduplication. This allowed us to study the events that occur during the transition from the mitotic cycle to the first endocycle. With giant cell differentiation, the cells switched cyclin D isoform expression from D3 to D1 and altered several checkpoint functions, acquiring a relative insensitivity to DNA-damaging agents and a coincident serum independence. The initiation of S phase during endocycles appeared to involve cycles of synthesis of cyclins E and A, and termination of S was associated with abrupt loss of cyclin A and E. Both cyclins were absent from gap phase cells, suggesting that their degradation may be necessary to allow reinitiation of the endocycle. The arrest of the mitotic cycle at the onset of endoreduplication was associated with a failure to assemble cyclin B/p34cdk1complexes during the first endocycle. In subsequent endocycles, cyclin B expression was suppressed. Together these data suggest several points at which cell cycle regulation could be targeted to shift cells from a mitotic to an endoreduplicative cycle.

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Tài liệu tham khảo

Alexander C.M., 1996, Development, 122, 1723, 10.1242/dev.122.6.1723

Barlow P.W., 1972, J. Embryol. Exp. Morphol., 27, 447

Basco R.D., 1995, Mol. Cell. Biol., 15, 5030, 10.1128/MCB.15.9.5030

Carney E.W., 1993, Mol. Reprod. Dev., 34, 357, 10.1002/mrd.1080340403

Chapman D.L., 1992, Mol. Reprod. Dev., 33, 259, 10.1002/mrd.1080330305

Chapman D.L., 1993, Development, 118, 229, 10.1242/dev.118.1.229

Cocker J.H., 1996, Nature, 379, 180, 10.1038/379180a0

Cross J.C., 1995, Development, 121, 2513, 10.1242/dev.121.8.2513

Cross J.C., 1994, Science, 266, 1508, 10.1126/science.7985020

Damjanov I., 1994, Biochem. Biophys. Res. Commun., 201, 994, 10.1006/bbrc.1994.1800

10.1091/mbc.7.2.209

Duronio R.J., 1995, Genes Dev., 9, 1456, 10.1101/gad.9.12.1456

Fantl V., 1995, Genes Dev., 9, 2364, 10.1101/gad.9.19.2364

Faria T.N., 1991, Biol. Reprod., 44, 327, 10.1095/biolreprod44.2.327

Faria T.N., 1991, Endocrinology, 129, 2895, 10.1210/endo-129-6-2895

Gardner R L., 1993, J. Exp. Zool., 265, 54, 10.1002/jez.1402650108

Goldstein L.S., 1975, Rad. Res., 62, 276, 10.2307/3574220

Grafi G., 1995, Science, 269, 1262, 10.1126/science.269.5228.1262

Hamlin G.P., 1994, Endocrinology, 134, 2390, 10.1210/endo.134.6.8194465

Hamlin G.P., 1995, Endocrinology, 136, 322, 10.1210/endo.136.1.7828548

Hartman T.P., 1995, Chromosome Res., 3, 271, 10.1007/BF00713064

Hoffman L.H., 1993, J. Exp. Zool., 266, 559, 10.1002/jez.1402660607

Hunt T., 1992, J. Cell Biol., 116, 707, 10.1083/jcb.116.3.707

King R.W., 1994, Cell, 79, 563, 10.1016/0092-8674(94)90542-8

Knoblich J.A., 1994, Cell, 77, 107, 10.1016/0092-8674(94)90239-9

Koff A., 1992, Science, 257, 1689, 10.1126/science.1388288

Lalande M., 1990, Exp. Cell Res., 186, 332, 10.1016/0014-4827(90)90313-Y

Lilly M., 1996, Genes Dev., 10, 2514, 10.1101/gad.10.19.2514

Malinowski S., 1994, Folia Histochem. Cytobiol., 32, 137

Matsushime H., 1991, Cell, 65, 701, 10.1016/0092-8674(91)90101-4

Morgan D.O., 1995, Nature, 374, 131, 10.1038/374131a0

Newman-Smith E., 1997, Dev. Genet., 20, 1, 10.1002/(SICI)1520-6408(1997)20:1<1::AID-DVG1>3.0.CO;2-B

Pathak S., 1994, In Vivo, 8, 843

Piatti S., 1996, Genes Dev., 10, 1516, 10.1101/gad.10.12.1516

Pines J., 1991, J. Cell Biol., 115, 1, 10.1083/jcb.115.1.1

Reponen P., 1994, Ann. N.Y. Acad. Sci., 732, 472, 10.1111/j.1749-6632.1994.tb24789.x

Rinkenberger J.L., 1997, Dev. Genet., 21, 6, 10.1002/(SICI)1520-6408(1997)21:1<6::AID-DVG2>3.0.CO;2-B

Sauer K., 1995, Genes Dev., 9, 1327, 10.1101/gad.9.11.1327

Shida M.M., 1993, Mol. Endocrinol., 7, 181

Sicinski P., 1995, Cell, 82, 621, 10.1016/0092-8674(95)90034-9

Sutherland A.E., 1993, Development, 119, 1175, 10.1242/dev.119.4.1175

Sweeney C., 1996, Development, 122, 53, 10.1242/dev.122.1.53

Wang Z., 1995, Blood, 86, 3783, 10.1182/blood.V86.10.3783.bloodjournal86103783

Watson P.A., 1991, Cytometry, 12, 242, 10.1002/cyto.990120306

Wilhide C.C., 1995, Blood, 86, 294, 10.1182/blood.V86.1.294.bloodjournal861294

Yamamoto T., 1994, J. Biol. Chem., 269, 6517, 10.1016/S0021-9258(17)37402-1

Zhang Y., 1996, J. Biol. Chem., 271, 4266, 10.1074/jbc.271.8.4266

Zhu D., 1997, Development, 124, 3007, 10.1242/dev.124.15.3007

Zybina E.V., 1970, Tsitologiia, 12, 1081

Zybina E.V., 1970, Tsitologiia, 12, 585

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Thông tin xuất bản

Molecular Biology of the Cell

Tập 9 Số 4

795-807

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