Mechanisms for Contractile Effect of Dai-kenchu-to in Isolated Guinea Pig Ileum
Tóm tắt
The mechanisms by which Dai-kenchu-to (TJ-100), a kampo medicine, enhances gastrointestinal motility was investigated using isolated guinea pig ileum. TJ-100 induced contractions accompanied by autonomous contraction at a concentration of more than 3 × 10−4 g/ml in a dose-related manner. The TJ-100-induced ileal contraction was suppressed by atropine and tetrodotoxin, but not by hexamethonium. This effect was partially suppressed in the presence of high concentrations of ICS 205-930, a serotonin 4 (5-HT4) receptor antagonist. In addition, TJ-100 showed an acetylcholine (ACh) -releasing action in the smooth muscle tissues of ileum. These results suggest that contractile response induced by TJ-100 is partially mediated by ACh released from the cholinergic nerve endings and that 5-HT4 receptors would be involved in the effect of TJ-100.
Tài liệu tham khảo
Horie Y, Kato K, Hamano K: An overview of our surgeryoriented kampo. J Tokyo Women's Med Coll 65(extra):43–51, 1995
Furukawa Y, Shiga Y, Hanyu N, Hashimoto Y, Mukai H, Nishikawa K, Aoki T: Effect of chinese herbal medicine on gastrointestinal motility and bowel obstruction. Jpn J Gastroenterol Surg 28(4):956–960, 1995
Hayakawa T, Kase Y, Saito K, Hashimoto K, Ishige A, Komatsu Y and Sasaki H: Effects of Dai-kenchu-to on intestinal obstruction following laparotomy. J Smooth Muscle Res 35: 47–54, 1999
Kurosawa S, Nishikawa S, Kaneko M, Nakamura T: The herbal medicine Dai-kenchu-to contracts guinea pig colon muscle while relaxing the gastric muscle-an evidence of new therapy for paralytic ileus. Gastroenterology 112:A768, 1997
Kurosawa S, Nishikawa S, Kaneko M, Ogiwara S, Ro S, Nakamura T, Saito K, Hayakawa T, Kase Y, Ishige A: The herbal medicine Dai-kenchu-to contracts guinea pig distal colon muscle through acetylcholine release. Gastroenterology 114:A782, 1998
Hayakawa T, Kase Y, Saito K, Hashimoto K, Ishige A, Komatsu Y, Sasaki H: Pharmacological studies on the effect of Dai-kenchu-to on spontaneous contraction of isolated rabbit jejunum. J Smooth Muscle Res 35:55–62, 1999
Wu ZC, Kisslinger SD, Gaginella TS: Functional evidence for the presence of cholinergic nerve endings in the colonic mucosa of the rat. J Pharmacol Exp Ther 221:664–669, 1982
Takahashi T, Kurosawa S, Wiley JW, Owyang C: Mechanism for the gastrokinetic action of domperidone. Gastroenterology 101:703–710, 1991
Itokawa H, Mihashi S, Watanabe K, Matsumoto H, Hamanaka T: Studies on the constituents of crude drugs having inhibitory activity against contraction of the ileum caused by histamine or barium chloride (1) screening test for the activity of commercially available crude drugs and the related plant materials. Jpn J Pharmacognosy 37(3):223–228, 1983
Kasahara Y, Saito E, Hikino H: Pharmacological actions of Pinellia tubers and Zingiber rhizomes. Jpn J Pharmacognosy 37(1):73–83, 1983
Takagi K, Saito H, Nabata H: Pharmacological studies of panax ginseng root: Estimation of pharmacological actions of panax ginseng root. Jpn J Pharmacol 22:245–259, 1972
Taniyama K, Nakayama S, Takeda K, Matsuyama S, Shirakawa J, Sano I, Tanaka C: Cisapride stimulates motility of the intestine via the 5-hydroxytryptamine receptors. J Pharmacol Exp Ther 258:1098–1104, 1991
Suzuki T, Nakata M, Nakamura T, Itoh Z: Effect of cisapride on contractile activity of the gastrointestinal tract. Jpn J Smooth Muscle Res 21:139–149, 1985
Nakayama S, Neya T, Yamasato T, Takaki M, Itano N: Effects of cisapride on the motility of digestive tract in dogs and guinea pigs. Jpn J Smooth Muscle Res 21:1–9, 1985
Fujii K, Okajima M, Kawahori K: Effect of cisapride on the cholinergic control mechanisms of gastrointestinal motility in dogs.Jpn J Smooth Muscle Res 24:1–12, 1988
Nagakura Y, Kadowaki M, Tokoro K, Tomoi M, Mori J, Kohsaka M: Pharmacological characterization of FK1052, a dihydropyridoindole derivative, as a new serotonin 3 and 4 dual receptor antagonist. J Pharmacol Exp Ther 265:752–758, 1993
Craig DA, Clarke DE: Pharmacological characterization of a neuronal receptor for 5-hydroxytryptamine in guinea pig ileum with properties similar to the 5-hydroxytryptamine4 receptor. J Pharmacol Exp Ther 252:1378–1386, 1990
Meulemans AL, Schuurkes JAJ: Is the action of cisapride on the guinea-pig ileum mediated via 5-HT4 receptors? Eur J Pharmacol 212:51–59, 1992
Kilbinger H, Wolf D: Effects of 5-HT4 receptors stimulation on basal and electrically evoked release of acetylcholine from guinea-pig myenteric plexus. Naunyn Schmiedebergs Arch Pharmacol 345:270–275, 1992