Phosphocreatine and ATP concentrations increase during flow-stimulated metabolism in a non-contracting muscle

The gracilis muscle was excised from cold-acclimated rats, placed in vitro, and simultaneously perfused via its artery by high pO2 medium and superfused by low pO2 medium. With a doubling of the perfusion rate (from 50 to 100 μl/min) phosphocreatine and ATP increased by 39% and 44%, respectively.