Genetic mapping of bph20(t) and bph21(t) loci conferring brown planthopper resistance to Nilaparvata lugens Stål in rice (Oryza sativa L.)

Euphytica - Tập 183 - Trang 161-171 - 2011
L. Yang1,2,3, R. B. Li1,3, Y. R. Li1, F. K. Huang2, Y. Z. Chen1,3, S. Sh. Huang2, L. F. Huang2, Ch. Liu1, Z. F. Ma1, D. H. Huang1, J. J. Jiang2
1Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning, China
2Plant Protection Research Institution, Guangxi Academy of Agricultural Sciences, Nanning, China
3Guangxi Key Laboratory of Subtropical Bioresource Conversation and Utilization and College of Agriculture, Guangxi University, Nanning, China

Tóm tắt

Brown planthopper(BPH) is one of the most serious and destructive insect pests of rice in most rice growing regions of the world. In this study, two major resistance genes against BPH have been identified in an Oryza rufipogon (Griff.) introgression rice line, RBPH54. Inheritance of the BPH resistance in RBPH54 was studied by screening the resistance in parents, F1, F2 and BC1 generations against BPH biotype 2. A population of BC3F2 lines was developed and SSR markers were employed for the gene mapping, and new markers were designed for fine mapping of the resistance genes, while sequence information of BAC/PAC clones was used to construct physical maps of the genes. The results showed that the BPH resistance in RBPH54 was governed by recessive alleles at two loci, tentatively designated as bph20(t) and bph21(t). The locus bph20(t) was fine mapped to the short arm of chromosome 6 about 2.7 cM to the upper marker RM435 and 2.5 cM to lower marker RM540 and in a 2.5 cM region flanked by two new SSR markers BYL7 and BYL8 which were developed in the present study. The other BPH resistance locus bph21(t) was initially mapped to a region 7.9 cM to upper marker RM222 and 4.0 cM to lower marker RM244 on the short arm of chromosome 10. For physical mapping, the bph20(t)-linked markers were landed on BAC/PAC clones of the reference cv., Nipponbare, released by the International Rice Genome Sequencing Project. The bph20(t) locus was physically defined to an interval of about 75 kb with clone P0514G1. Identification and location of these two genes in the present study have diversified the BPH resistance gene pool, which give benefit to the development of resistant rice cultivars, and the linkage PCR-based SSR markers for the bph20(t) and bph21(t) genes would help realize the application of the genes in rice breeding through marker-assisted selection.

Tài liệu tham khảo

Athwal DS, Pathak MD, Bacalangco EH, Pura CD (1971) Genetics of resistance to brown planthopper and green leafhopper in Oryza sativa L. Crop Sci 11:747–750 Beavis WD, Grant D, Albersen M, Fincher R (1991) Quantitative trait loci for plant height in four maize populations and their associations with qualitative genetic loci. Theor Appl Genet 83:141–145 Brar DS, Khush GS (1997) Alien introgression in rice. Plant Mol Biol 35:35–47 Chen F, Tan YJ, Shuai YY (1989) Identification of resistance of wild rice germplasm resources to brown planthopper nilaparvata lugens (ståal) in Guangdong province. J Plant Prot 1:12–26 Chen JW, Wang L, Pang XF, Pan QH (2006) Genetic analysis and fine mapping of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph19 (t). Mol Gen Genomics 275:321–329 Dyck VA, Thomas B (1979) The brown planthopper problem. In: International Rice Research Institute (ed) Brown planthopper: threat to rice production in Asia. IRRI, Los Banos, Philippines, pp 3–17 Feuer R (1976) Biotype II brown planthopper in the Philippines. Int Rice Res Newsl 1(1):15 Heinrichs EA (1986) Perspectives and directions for the continued development of insect resistant rice varieties. Agric Ecosyst Environ 18:9–36 Hirabayashi H, Ogawa T (1995) RFLP mapping of Bph-1 (brown planthopper resistance gene) in rice. Breed Sci 45:369–371 Hirabayashi H, Ogawa T (1999) Identification and utilization of DNA markers linked to genes for resistance to brown planthopper (BPH) in rice. Recent Adv Breed Sci 41:71–74 (in Japanese) Hirabayashi H, Angeles ER, Kaji R, Ogawa T, Brar DS, Khush GS (1998) Identification of brown planthopper resistance gene derived from O. officinalis using molecular markers in rice. Breed Sci Suppl 48:82 (in Japanese) Holt J, Chancellor TCB, Reynolds DR, Tiongco ER (1996) Risk assessment for rice planthopper and tungro disease outbreaks. Crop Prot 15(4):359–368 Huang CF (2003) Development of position specific microsatellite markers and molecular mapping of insect resistant genes in rice (Oryza sativa L.). MS Thesis, South China Agric Univ, Guangzhou, pp 31–40 (in Chinese) Huang Z, He G, Shu L, Li X, Zhang Q (2001) Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet 102:929–934 IRRI (1981–1982) A brown planthopper population that feed on IR36. IRRI Ann Rep 56–58 IRRI (1988) Standard evaluation system for rice. International Rice Research Institute, Manila Ishii T, Brar DS, Multani DS, Khush GS (1994) Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativa. Genome 37:217–221 Jena KK, Pasalu IC, Rao YK, Varalaxmi Y, Krishnaiah K, Khush GS (2002) Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.). Euphytica 129:81–88 Jena KK, Jeung JU, Lee JH, Choi HC, Brar DS (2005) High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t) and marker-assisted selection for BPH resistance in rice (Oryza sativa L.). Theor Appl Genet 111:127–135 Kabir MA, Khush GS (1988) Genetic analysis of resistance to brown planthopper in rice, Oryza sativa L. Plant Breed 100:54–58 Kawaguchi M, Murata K, Ishii T, Takumi S, Mori N, Nakamura C (2001) Assignment of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph4 to the rice chromosome 6. Breed Sci 51:13–18 Khush GS, Rezaul-Karim ANM, Angeles ER (1985) Genetics of resistance of rice cultivar ARC10550 to Bangladesh brown planthopper biotype. J Genet 64:121–125 Lakshminarayana A, Khush GS (1977) New genes for resistance to the brown planthopper in rice. Crop Sci 17:96–100 Li Q, Luo SY, Huang RQ, Wei SM, Wu MX, Li DY (1990) Study on resistance of an O. officinalis collection from the Guangxi province of China. In: Collection of research papers on wild rice. Science and Technology Press of China,Beijing, pp 40–43 Li RZh, Ding JH, Hu WG (1996) Brown planthopper and its population management. Fudai Univ Publ Co, Shanghai, pp 239–242 Li RB, Qin XY, Wei SM, Huang FK, Li Q, Luo SY (2002) Identification and genetics of resistance against brown planthopper in a derivative of wild rice. Oryza rufipogon Griff. J Genet Breed 56:29–36 Li RB, LSh Li, Wei SM, Wei YP, YZh Chen, Bai DL, Yang L, Huang FK (2006) The evaluation and utilization of new genes for brown planthopper resistance in common wild rice (Oryza rufipogon Griff.). Mol Plant Breed 4(3):365–371 Lincoln S, Daly M, Lander ES (1992) Constructing genetic maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report, 2nd edn, Whitehead Institute, Cambridge Liu GQ, Yan HH, Fu Q, Qian Q, Zhang ZT, Zhai WX, Zhu LH (2001) Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri. Chin Sci Bull 46:1459–1462 Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832 Monna L, Lin HX, Kojima S, Sasaki T, Yano M (2002) Genetic dissection of a genomic region for quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice. Theor Appl Genet 104:772–778 Murata K, Fujiwara M, Kaneda C, Takumi S, Mori N, Nakamura C (1998) RFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph2 of indica rice introgressed into a japonica breeding line ‘Norin-PL4’. Genes Genet Syst 73:359–364 Murata K, Fujiwara M, Murai H, Takumi S, Mori N, Nakamura C (2001) Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12. Cereal Res Commun 29:245–250 Murray MG, Thompson WK (1980) Rapid isolation of highmolecular-weight plant DNA. Nucleic Acids Res 8:4321–4325 Nemoto H, Ikeda R, Kaneda C (1989) New genes for resistance to brown planthopper, Nilaparvata lugens Stål, in rice. Jpn J Breed 39:23–28 Pan QH, Hu ZD, Takatoshi T (2003) Fine mapping of the blast resistance gene Pi15, linked to Pii, on rice chromosome 9. Acta Bot Sin 45:871–877 Pang HH, ChB Chen (2002) Chinese germplasm of wild rice. Guangxi Sci Publ Co, Nanning, pp 4–28 Pathak MD (1972) Resistance to insect pests in rice varieties. In: International Rice Research Institute (ed) Rice breeding. IRRI, Los Baños, Philippines, pp 325–341 Ramalingam J, Vera-Cruz CM, Kukreja K, Chittoor JM, Wu JL, Lee SW, Baraoidan M, George ML, Cohen MB, Hulbert SH, Leach JE, Leung H (2003) Candidate defense genes from rice, barley, and maize and their association with qualitative and quantitative resistance in rice. Mol Plant Microbe Interact 16:14–24 Renganayaki K, Fritz AK, Sadasivam S, Pammi S, Harrington SE, McCouch SR, Kumar SM, Reddy AS (2002) Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from Oryza officinalis into cultivated rice O. sativa. Crop Sci 42:2112–2117 Sogawa K (1982) The rice brown planthopper: feeding physiology and host plant interactions. Annu Rev Entomol 27:49–73 Staokey JH (1975) The problem of brown planthopper (Nilaparvata lugens Stål) on rice in Solomon Islands. Rice Entomol Newsl 2:37 Wang GL, Mackill DJ, Bonman JM, McCouch SR, Champoux MC, Nelson RJ (1994) RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136:1421–1434 Wang GR, Lai FX, Fu Q, Zhang ZT, Guo LF (1999) Virulent shift in populations of Nilaparvata lugens (Homptera: Delphacidae). Chin J Rice Sci 13:229–232 (in Chinese) Wu JT, Heinrichs EA, Medrano FG (1986) Resistance of wild rice, Oryza spp., to the brown planthopper, Nilaparvata Lugens (Homoptera: Delphacidae). Environ Entomol 15:648–653 Xu XF, Mei HW, Luo LJ, Cheng XN, Li ZK (2002) RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens). Theor Appl Genet 104:248–253 Yang HY, Ren X, Weng QM, Zhu LL, He GC (2002) Molecular mapping and genetic analysis of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene. Heredity 136:39–43 Yang Z, Sun X, Wang S, Zhang Q (2003) Genetic and physical mapping of a new gene for bacterial blight resistance in rice. Theor Appl Genet 106:1467–1472 Yang HY, You AQ, Yang ZF, Zhang FT, He RF, Zhu LL, He GC (2004) High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theor Appl Genet 110:182–191 Yang L, Li RB, Li YR (2005) Preliminary mapping the genes conferring resistant to brown planthopper Nilaparvata lugens in rice. Mol Plant Breed 3(6):807–809 Zhu LL, Zhu CL, Weng QM, Huang Z, He GC (2004) Research progress on brown planthopper resistance genes in rice. Hubei Agric Sci 1:19–24 (in Chinese)