Transport of influenza virus envelope proteins from the Golgi complex to the apical plasma membrane in MDCK cells: pH‐Controlled interaction with a cycling receptor is not involved

FEBS Letters - Tập 249 - Trang 407-410 - 1989
Phillipa U. Daniels-Holgate1, J.Michael Edwardson1
1Department of Pharmacology, University of Cambridge, Hills Road, Cambridge CB2 2QD, England

Tóm tắt

In influenza virus‐infected monolayers of the epithelial cell line MDCK the viral envelope proteins, haemagglutinin and neuraminidase, are targetted specifically to the apical surface. In this study we have tested the hypothesis that the polarized delivery of these proteins to the plasma membrane involves the operation of a receptor that cycles between the trans Golgi network and the plasma membrane, binding the proteins at low pH in the former compartment and releasing them at normal extracellular pH in the latter. The hypothesis predicts that apical, but not basolateral, low pH would eventually delay or block delivery of the proteins to the plasma membrane. We found that basolateral low pH in fact had the more profound effect, in line with its greater effect on intracellular pH. We conclude that the hypothesis is not valid, and that low extracellular pH causes its effect on protein transport by changing intracellular pH.

Tài liệu tham khảo

10.1126/science.2945253 10.1016/0092-8674(80)90233-0 10.1073/pnas.83.7.2100 10.1038/329632a0 10.1073/pnas.84.11.3738 10.1073/pnas.84.9.2756 10.1083/jcb.105.6.2735 10.1016/0092-8674(85)90212-0 10.1016/S0021-9258(18)66848-6 10.1083/jcb.101.6.2253 10.1016/0092-8674(83)90052-1 10.1042/bj2520693 10.1016/0014-5793(89)81161-5 10.1002/j.1460-2075.1987.tb02691.x 10.1016/S0021-9258(18)66925-X 10.1016/S0021-9258(18)66926-1 10.1083/jcb.105.2.679
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FEBS Letters

Tập 249

407-410

Thông tin tác giả