Phillipa U. Daniels-Holgate1, J.Michael Edwardson1
1Department of Pharmacology, University of Cambridge, Hills Road, Cambridge CB2 2QD, England
Tóm tắt
In influenza virus‐infected monolayers of the epithelial cell line MDCK the viral envelope proteins, haemagglutinin and neuraminidase, are targetted specifically to the apical surface. In this study we have tested the hypothesis that the polarized delivery of these proteins to the plasma membrane involves the operation of a receptor that cycles between the trans Golgi network and the plasma membrane, binding the proteins at low pH in the former compartment and releasing them at normal extracellular pH in the latter. The hypothesis predicts that apical, but not basolateral, low pH would eventually delay or block delivery of the proteins to the plasma membrane. We found that basolateral low pH in fact had the more profound effect, in line with its greater effect on intracellular pH. We conclude that the hypothesis is not valid, and that low extracellular pH causes its effect on protein transport by changing intracellular pH.
