A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2

American Association for the Advancement of Science (AAAS) - Tập 369 Số 6504 - Trang 650-655 - 2020
Xiangyang Chi1, Renhong Yan2, Jun Zhang1, Guanying Zhang1, Yuanyuan Zhang2, Meng Hao1, Zhe Zhang1, Pengfei Fan1, Yunzhu Dong1, Yilong Yang1, Zhengshan Chen1, Yingying Guo2, Jinlong Zhang1, Yaning Li3, Xiaohong Song1, Yi Chen1, Lu Xia2, Ling Fu1, Lihua Hou1, Junjie Xu1, Changming Yu1, Jianmin Li1, Qiang Zhou2, Wei Chen1
1Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing 100071, China.
2Key Laboratory of Structural Biology of Zhejiang Province, Institute of Biology, Westlake Institute for Advanced Study, School of Life Sciences, Westlake University, Hangzhou 310024, Zhejiang Province, China.
3Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China

Tóm tắt

Hitting SARS-CoV-2 in a new spot A key target for therapeutic antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the spike protein, a trimeric protein complex with each monomer comprising an S1 and an S2 domain that mediate binding to host cells and membrane fusion, respectively. In addition to the receptor binding domain (RBD), S1 has an N-terminal domain (NTD). In searching for neutralizing antibodies, there has been a focus on the RBD. Chi et al. isolated antibodies from 10 convalescent patients and identified an antibody that potently neutralizes the virus but does not bind the RBD. Cryo–electron microscopy revealed the epitope as the NTD. This NTD-targeting antibody may be useful to combine with RBD-targeting antibodies in therapeutic cocktails. Science , this issue p. 650

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