The ArsR protein is a <i>trans</i>‐acting regulatory protein

Molecular Microbiology - Tập 5 Số 6 - Trang 1331-1336 - 1991
Jiayuan Wu1, Barry P. Rosen1
1Department of Biochemistry Wayne State University School of Medicine Detroit, Michigan 48201, USA

Tóm tắt

SummaryThe arsR gene encodes the regulatory protein of the plasmid‐encoded arsenical resistance operon. A series of in‐frame fusions was constructed between the C‐terminally truncated arsR gene and the coding region for the mature form of β‐lactamase (blaM). Fusions containing most of the arsR gene were still inducible by arsenicals. Fusions containing less than 102 residues of the 117‐residue ArsR protein were constitutive. When a wild‐type arsR gene was placed in trans, the constitutive constructs were again inducible. The results demonstrate that the ArsR protein is a trans‐acting regulatory protein which controls its own expression.

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Tài liệu tham khảo

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Molecular Microbiology

Tập 5 Số 6

1331-1336

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