Proteins from the prokaryotic nucleoid Interaction of nucleic acids with the 15 kDa Escherichia coli histone‐like protein H‐NS

FEBS Letters - Tập 229 Số 1 - Trang 197-202 - 1988
K. Andreas Friedrich1, Claudio O. Gualerzi2,1, M. Lammi3,2, Maria Adele Losso3, Cynthia L. Pon1
1Max-Planck-Institut für Molekulare Genetik (Abt. Wittmann), D-1000 Berlin 33, Germany
2Laboratory of Genetics, DBC, University of Camerino, I-62032 Camerino (MC Italy
3Department of Cell Biology, University of Calabria, I-87036 Arcavacata di Rende, Italy

Tóm tắt

The interaction between nucleic acids and Escherichia coli H‐NS, an abundant 15 kDa histone‐like protein, has been studied by affinity chromatography, nitrocellulose filtration and fluorescence spectroscopy. Intrinsic fluorescence studies showed that the single Trp residue of H‐NS (position 108) has a restricted mobility and is located within an hydrophobic region inaccessible to both anionic and cationic quenchers. Binding of H‐NS to nucleic acids, however, results in a change of the microenvironment of the Trp residue and fluorescence quenching; from the titration curves obtained with addition of increasing amounts of poly(dA)‐poly(dT) and poly(dC)‐poly(dG) it can be estimated that an H‐NS dimer in 1.5 × SSC binds DNA with an apparent K a−1.1 × 104 M−1· bp−1. H‐NS binds to double‐stranded DNA with a higher affinity than the more abundant histone‐like protein NS(HU) and, unlike NS, prefers double‐stranded to single‐stranded DNA and DNA to RNA; both monovalent and divalent cations are required for optimal binding.

Từ khóa


Tài liệu tham khảo

10.1007/978-3-642-71266-1_10

10.1007/978-3-642-71266-1_11

10.1128/MMBR.51.3.301-319.1987

10.1007/BF00425687

10.1016/0378-1119(86)90129-0

10.1007/BF00329674

10.1007/978-1-4684-8730-5_48

10.1093/nar/4.8.2725

10.1093/nar/12.13.5321

Falconi M., 1988, Mol. Microbiol.

Pon C.L., 1988, Mol. Gen. Genet.

Pawlik R.T., 1981, Biochem. Int., 2, 421

10.1111/j.1432-1033.1982.tb06913.x

10.1016/0076-6879(79)59125-3

10.1016/0014-5793(85)80753-5

10.1016/0006-291X(84)90895-7

10.1016/0014-5793(78)80446-3

10.1016/0092-8674(82)90269-0

Thông tin
Thông tin xuất bản

FEBS Letters

Tập 229 Số 1

197-202

Thông tin tác giả