Cyclin‐dependent kinase 4 is a novel target in micoRNA‐195‐mediated cell cycle arrest in bladder cancer cells

FEBS Letters - Tập 586 - Trang 442-447 - 2012
Yiwei Lin1, Jian Wu1, Hong Chen1, Yeqing Mao1, Yunfu Liu1, Qiqi Mao1, Kai Yang1, Xiangyi Zheng1, Liping Xie1
1Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China

Tóm tắt

miRNAs are a class of small‐noncoding RNAs capable of negatively regulating gene expression. Here, we found that miR‐195 is down‐regulated in human bladder cancer tissue versus normal adjacent tissue. To better characterize the role of miR‐195 in bladder cancer, we conducted gain of function analysis by transfecting bladder cancer cell line T24 with chemically synthesized miR‐195 mimic. We identified CDK4, an early G1 cell cycle regulator, as a novel target of miR‐195. Selective over‐expression of miR‐195 could induce G1‐phase arrest in T24 cells, and subsequently inhibit T24 cell growth. These findings indicate that miR‐195 could be a potential tumor suppressor in bladder cancer.

Tài liệu tham khảo

10.1007/s00345-009-0383-3 10.1038/sj.bjc.6601813 10.1111/j.1349-7006.2008.00735.x 10.1016/S0092-8674(04)00045-5 10.1016/j.cell.2009.01.002 10.1590/S1415-47572011000300001 Zhao L. Chen X. Cao Y. (2011). New role of microRNA: carcinogenesis and clinical application in cancer. Acta Biochim Biophys Sin (Shanghai). 10.1016/j.urolonc.2007.01.019 10.1016/j.juro.2008.10.149 10.1038/onc.2009.395 10.1002/ijc.24390 10.1371/journal.pone.0018286 10.1158/0008-5472.CAN-07-0533 10.1590/S0100-879X2007001100003 10.1158/1078-0432.CCR-07-0666 10.1002/hep.22919 10.1016/j.bbrc.2010.08.046 10.1158/0008-5472.CAN-07-5032 10.1038/37126 10.1038/nrurol.2011.144 10.1002/path.2806 10.1080/13547500802646572 10.1016/j.pharmthera.2011.04.006 10.1016/j.eururo.2011.01.044 10.1016/j.urolonc.2009.08.024 10.1007/s11255-009-9584-3 10.1002/jcp.22598 10.1155/2009/106378 10.1101/gad.1033002 10.1128/MCB.24.17.7538-7547.2004 Wang X., 2011, Efficient down‐regulation of CDK4 by novel lipid nanoparticle‐mediated siRNA delivery, Anticancer Res., 31, 1619
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FEBS Letters

Tập 586

442-447

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