Antioxidant and antiapoptotic effects of proanthocyanidin and ginkgo biloba extract against doxorubicin‐induced cardiac injury in rats

Cell Biochemistry and Function - Tập 31 Số 4 - Trang 344-351 - 2013
Noha A. El-Boghdady1
1Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt

Tóm tắt

Grape seed proanthocyanidins (GSPE) and ginkgo biloba extract (EGb761) are considered to have protective effects against several diseases. The cardiotoxicity of doxorubicin (DOX) has been reported to be associated with oxidative damage. This study was conducted to evaluate the cardioprotective effects of GSPE and EGb761 against DOX‐induced heart injury in rats. DOX was administered as a single i.p. dose (20 mg kg–1) to adult male rats. DOX‐intoxicated rats were orally administered GSPE (200 mg kg–1 day–1) or EGb761 (100 mg kg–1 day–1) for 15 consecutive days, starting 10 days prior DOX injection. DOX‐induced cardiotoxicity was evidenced by a significant increase in serum aspartate transaminase (AST), creatine phosphokinase isoenzyme (CK‐MB), lactate dehydrogenase (LDH), total cholesterol (TC) and triglyceride (TG) activities and levels. Increased oxidative damage was expressed by the depletion of cardiac reduced glutathione (GSH), elevation of cardiac total antioxidant (TAO) level and accumulation of the lipid peroxidation product, malondialdehyde (MDA). Significant rises in cardiac tumour necrosis factor‐alpha (TNF‐α) and caspase‐3 levels were noticed in DOX‐intoxicated rats. These changes were ameliorated in the GSPE and EGb761‐treated groups. Histopathological analysis confirmed the cardioprotective effects of GSPE and EGb761. In conclusion, GSPE and EGb761 mediate their protective effect against DOX‐induced cardiac injury through antioxidant, anti‐inflammatory and antiapoptotic mechanisms. Copyright © 2012 John Wiley & Sons, Ltd.

Từ khóa


Tài liệu tham khảo

10.1111/j.1365-2141.2005.05759.x

10.1056/NEJM199809243391307

10.1006/mgme.2000.3043

10.1111/j.1749-6632.2002.tb02922.x

Fine AM, 2000, Oligomeric proanthocyanidin complexes: history, structure, and phytopharmaceutical applications, Altern Med Rev, 5, 144

10.1139/y05-018

10.1016/S0278-6915(02)00006-6

10.1016/0140-6736(92)93158-J

10.1006/jmcc.1996.0316

10.1002/ptr.1973

10.1111/j.1478-3231.2007.01463.x

10.1016/S0024-3205(00)00741-4

10.1046/j.1472-8206.2003.00156.x

10.1016/S1043-6618(03)00185-3

Timioğlu O, 1999, The effect of EGb 761 on the doxorubicin cardiomyopathy, Res Commun Mol Pathol Pharmacol, 106, 181

10.1093/ajcp/28.1.56

10.1093/clinchem/24.5.828

10.1016/0006-291X(65)90200-7

10.1056/NEJM196701052760107

10.1177/000456327301000125

10.1016/0003-2697(78)90342-1

Beutler E, 1963, Improved method for the determination of blood glutathione, J Lab Clin Med, 61, 882

10.1136/jcp.54.5.356

10.1016/S0021-9258(19)40049-5

10.1016/S0021-9258(18)47344-9

10.1016/S0021-9258(19)52451-6

Banchroft JD, 1996, Theory and practice of histological techniques

10.1016/S0959-8049(01)00050-8

Davies KJ, 1986, Redox cycling of anthracyclines by cardiac mitochondria: anthracycline radical formation by NADPH dehydrogenous, J Biol Chem, 261, 3060, 10.1016/S0021-9258(17)35746-0

10.1016/0005-2760(82)90251-X

10.1111/j.1600-0773.2001.890305.x

Joles JA, 1993, Plasma lipoproteins and renal apolipoproteins in rats with chronic adriamycin nephrosis, Nephrol Dial Transplant, 8, 831

10.1159/000188398

10.1254/jjp.39.323

10.1016/j.jnutbio.2004.06.008

10.1248/yakushi1947.113.10_718

10.1093/jn/130.9.2243

10.1021/jf048177q

10.1024/0300-9831.72.3.161

10.1016/j.carpath.2010.09.002

10.1002/biof.5520330307

10.1016/S1734-1140(10)70328-5

10.1007/BF02256052

10.1021/bi00447a056

10.1016/S0891-5849(98)00303-7

Thomas JA, 1994, Modern Nutrition in Health and Disease, 501

10.2174/0929867043365288

10.1006/abbi.1999.1525

10.1111/j.2042-7158.1988.tb05173.x

10.1152/ajpregu.1998.275.1.R212

Bagchi D, 1997, Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro, Res Commun Mol Pathol Pharmacol, 95, 179

10.1186/1471-2210-3-16

10.1016/j.ijcard.2010.04.008

10.1161/01.RES.79.2.162

10.1056/NEJM199704103361506

10.1006/jmcc.1999.1006

10.1006/taap.2002.9532

10.1006/phrs.2000.0769

Thông tin
Thông tin xuất bản

Cell Biochemistry and Function

Tập 31 Số 4

344-351

Thông tin tác giả