Mapping of quantitative trait loci controlling partial resistance against rust incited by Uromyces pisi (Pers.) Wint. in a Pisum fulvum L. intraspecific cross

Euphytica - Tập 175 - Trang 151-159 - 2010
E. Barilli1, Z. Satovic2, D. Rubiales1, A. M. Torres3
1Institute for Sustainable Agriculture, CSIC, Córdoba, Spain
2Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia
3IFAPA, Centro Alameda del Obispo, Córdoba, Spain

Tóm tắt

A quantitative trait loci (QTL) associated with resistance to pea rust, caused by the fungus Uromyces pisi (Pers.) Wint., has been identified in a F2 population derived from an intraspecific cross between two wild pea (Pisum fulvum L.) accessions, IFPI3260 (resistant) and IFPI3251 (susceptible). Both parental lines and all the segregating population displayed a fully compatible interaction (high infection type), which indicates absence of hypersensitive response. Nevertheless, differences on the percentage of symptomatic area of the whole plant (disease severity) were observed. A genetic map was developed covering 1283.3 cM and including 146 markers (144 random amplified polymorphic DNA (RAPDs) and two sequence tagged sites (STSs) markers) distributed in 9 linkage groups. A QTL explaining 63% of the total phenotypic variation was located in linkage group 3. RAPDs markers (OPY111316 and OPV171078) flanking this QTL should allow, after their conversion in SCARs, a reliable marker-assisted selection for rust resistance.

Tài liệu tham khảo

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