Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors

American Association for the Advancement of Science (AAAS) - Tập 258 Số 5083 - Trang 818-821 - 1992
Anne Kallioniemi1, Olli Kallioniemi1, Damir Sudar1, Denis Rutovitz2, Joe W. Gray1, F M Waldman1, Dan Pinkel1
1Division of Molecular Cytometry, Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94143.
2Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, United Kingdom

Tóm tắt

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.

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Tài liệu tham khảo

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American Association for the Advancement of Science (AAAS)

Tập 258 Số 5083

818-821

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