Visualization of Bile Homeostasis Using 1H‐NMR Spectroscopy as a Route for Assessing Liver Cancer

Lipids - Tập 44 Số 1 - Trang 27-35 - 2009
G. A. Nagana Gowda1, Narasimhamurthy Shanaiah1, Amanda Cooper2, Mary A. Maluccio2, Daniel Raftery3
1Department of Chemistry, Purdue University, West Lafayette, IN, 47907 USA
2Department of Surgery, Indiana University, Indianapolis, IN, 46202 USA
3Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA

Tóm tắt

AbstractChanges in bile synthesis by the liver or alterations in the enterohepatic circulation due to a variety of etiological conditions may represent a novel source of liver disease‐specific biomarkers. Bile from patients with liver diseases exhibited significant changes in the levels of glycine‐ and taurine‐conjugated bile acids, phospholipids, cholesterol and urea relative to non‐liver disease controls. Cholangiocarcinoma and non‐malignant liver diseases (NMLD) showed the most significant alterations. Further, hepatocellular carcinoma (HCC) could be differentiated from NMLD (p = 0.02), as well as non‐liver disease controls (p = 0.02) based on the amounts of bile acids, phospholipids and/or cholesterol. HCC also differed with cholangiocarcinoma although not significantly. Urea increases somewhat in non‐malignant liver disease relative to non‐liver disease controls, while the bile acids, phospholipids and cholesterol all decrease significantly. The ratio between some major bile metabolites also distinguished NMLD (p = 0.004–0.01) from non‐liver disease controls. This snapshot view of bile homeostasis, is obtainable from a simple nuclear magnetic resonance (NMR) approach and demonstrates the enormous opportunity to assess liver status, explore biomarkers for high risk diseases such as cancers and improve the understanding of normal and abnormal cellular functions.

Từ khóa


Tài liệu tham khảo

10.1016/j.cld.2008.03.007

10.1586/14737159.8.5.617

10.1007/s00216-006-0687-8

10.1002/cem.941

10.1007/s00216-006-0546-7

10.1002/rcm.2474

10.1007/s11306-006-0026-2

10.1002/ijc.20651

10.1177/153303460400300609

10.1021/pr070063h

10.1111/j.1432-2277.1998.tb00805.x

10.3748/wjg.v12.i30.4773

10.1067/msy.2003.142

10.1097/00042737-200507000-00007

10.1046/j.1440-1746.2000.02126.x

El‐Houseini ME, 2000, Evaluation of serum total bile acids in the diagnosis of hepatocellular carcinoma, J Egyptian Nat Cancer Inst, 12, 307

10.1111/j.1440-1746.1996.tb00064.x

10.1080/00365519209115502

10.3109/00365528209181055

10.1080/00365521.1976.12097147

10.1148/radiol.2292021156

10.1016/j.bbalip.2007.01.006

10.1007/s11745-006-5008-7

10.1007/s11745-006-5007-8

10.1007/s11745-005-1466-1

10.1002/mrm.20513

10.1080/00032710500260589

10.1002/nbm.829

Noël‐Paton D, 1886, Nature of the relationship of urea formation to bile secretion, J Anat Physiol, 20, 662

10.1002/mas.10046

10.1002/(SICI)1099-0801(199707)11:4<240::AID-BMC686>3.0.CO;2-6

10.1002/mas.20023

Thông tin
Thông tin xuất bản

Lipids

Tập 44 Số 1

27-35

Thông tin tác giả