Legumes in prairie restoration: evidence for wide cross-nodulation and improved inoculant delivery
Tóm tắt
Prairie restoration aims to create self-sustaining, resilient prairies that ameliorate biodiversity loss and soil deterioration associated with conversion of native grasslands to agriculture. Legumes are a key component of the nitrogen-limited prairie ecosystem. Evidence suggests that lack of suitable rhizobia may explain legume absence from restored prairies. This study explores effects of novel alternative inoculant delivery methods on: (a) prairie legume establishment, (b) soil biological properties, and (c) inoculant strain ability to nodulate the host over time. Alternative inoculation methods for seven legume species were tested in a replicated field experiment. Legume establishment, microbial biomass carbon and nitrogen, and rhizobial inoculant strain recovery were measured over a 3-year period. Legume species richness in the second growing season was enhanced by a soil-applied granular clay inoculant, while seed-applied powdered peat inoculation was generally ineffective. When Dalea rhizobia were recovered 3-year after planting, only 2 % from the seed-applied inoculation treatment identified with the inoculant strains, whereas this amount ranged from 53 to 100 % in the other inoculation treatments. Some legumes established unexpectedly effective symbioses with strains not originally intended for them. Results provide new insights on inoculation of native legumes, especially when a mix of seeds is involved and the restoration targets harsh environments.
Tài liệu tham khảo
Baer SG, Blair JM, Collins SL, Knapp AK (2003) Soil resources regulate productivity and diversity in newly established tallgrass prairie. Ecology 84:724–735
Bernal G, Tlusty B, de Jensen CE, van Berkum P, Graham PH (2004) Characteristics of rhizobia nodulating beans in the central region of Minnesota. Can J Microbiol 50:1023–1031
Betz RF, Lamp HF (1989) Species composition of old settler silt-loam prairies. In: Bragg TB, Stubbendieck J (eds) Proceedings of the Eleventh North American Prairie Conference. University of Nebraska, Lincoln, pp 33–39
Blumenthal DM, Jordan NR, Russelle MP (2003) Soil carbon addition controls weeds and facilitates prairie restoration. Ecol Appl 13:605–615
Brejda JJ, Moorman TB, Smith JL, Karlen DL, Allan DL, Dao TH (2000) Distribution and variability of surface soil properties at a regional scale. Soil Sci Soc Am J 64:974–982
Catroux G, Hartmann A, Revellin C (2001) Trends in rhizobial inoculant production and use. Plant Soil 230:21–30
Cloutier J, Laberge S, Prevost D, Antoun H (1996) Sequence and mutational analysis of the common nodBCIJ region of Rhizobium sp (Oxytropis arctobia) strain N33, a nitrogen-fixing microsymbiont of both arctic and temperate legumes. Mol Plant Microb In 9:523–531
Date RA (1991) Nodulation success and persistence of recommended inoculum strains for subtropical and tropical forage legumes in Northern Australia. Soil Biol Biochem 23:533–541
De Bruin JL, Pedersen P, Conley SP, Gaska JM, Naeve SL, Kurle JE, Elmore RW, Giesler LJ, Abendroth LJ (2010) Probability of yield response to inoculants in fields with a history of soybean. Crop Sci 50:265–272
Denton MD, Pearce DJ, Ballard RA, Hannah MC, Mutch LA, Norng S, Slattery JF (2009) A multi-site field evaluation of granular inoculants for legume nodulation. Soil Biol Biochem 41:2508–2516
Diatloff A (1969) The introduction of Rhizobium japonicum to soil by seed inoculation of non-host legumes and cereals. Aust J Exp Agric 9:357–360
Djupstrom B (1988) Minnesota’s prairies: past, present, and future. Rangelands 10:109–111
Finzi AC, Rodgers VL (2009) Bottom-up rather than top-down processes regulate the abundance and activity of nitrogen fixing plants in two Connecticut old-field ecosystems. Biogeochemistry 95:309–321
Graham PH (1963) Vitamin requirements of root nodule bacteria. J Gen Microbiol 30:345–248
Graham PH (2004) Inoculated legumes and revegetation/roadside plantings. Final report. Office of Research Services, Minnesota Department of Transportation, St. Paul
Graham PH (2005) Practices and issues in the inoculation of prairie legumes used in revegetation and restoration. Ecol Restor 23:186–194
Graham PH, Ballen KG, Montealegre C, Jones RK, Fischer B, Luque E (1999) Characterization of rhizobia associated with Dalea spp. in natural prairies and revegetation areas of Minnesota. In: Martinez E, Hernandez G (eds) Highlights of nitrogen fixation research. Kluwer/Plenum, New York, pp 69–75
Herridge DF (2008) Inoculation technology for legumes. In: Dilworth MJ, James EK, Sprent JI, Newton WE (eds) Nitrogen-fixing leguminous symbioses. Springer, Dordrecht, pp 77–115
Hille Ris Lambers J, Harpole WS, Tilman D, Knops J, Reich PB (2004) Mechanisms responsible for the positive diversity-productivity relationship in Minnesota grasslands. Ecol Lett 7:661–668
Horwath WR, Paul EA (1994) Microbial biomass. In: Weaver RW, Angle JS, Bottomley P J, Bezdicek DF, Smith MS, Tabatabai MA, and Wollam AG (eds) Methods of Soil Analysis, Part 2: Microbiological and Biochemical Properties. Soil Sci Soc Am J, Madison, WI, pp. 753–774
Hurse LS, Date RA (1992) Competitiveness of indigenous strains of Bradyrhizobium on Desmodium intortum cv Greeenleaf in three soils of south east Queensland. Soil Biol Biochem 24:41–50
Inouye RS, Huntly NJ, Tilman D, Tester JR, Stillwell M, Zinnel KC (1987) Old-field succession on a Minnesota sand plain. Ecology 68:12–26
Jha PK, Nair S, Gopinathan MC, Babu CR (1995) Suitability of rhizobia-inoculated wild legumes Argyrolobium fraccidum, Astragalus graveolens, Indigofera gangetica and Lespedeza stenocarpa in providing a vegetational cover in an unreclaimed limestone quarry. Plant Soil 177:139–149
Kempel A, Brandl R, Schaedler M (2009) Symbiotic soil microorganisms as players in aboveground plant-herbivore interactions - the role of rhizobia. Oikos 118:634–640
Kindscher K, Tieszen LL (1998) Floristic and soil organic matter changes after five and thirty-five years of native tallgrass prairie restoration. Restor Ecol 6:181–196
Kuratomi M (2003) Assessing soil quality in alternative and conventional cropping systems. MS Thesis. University of Minnesota, St. Paul
Larson JL, Siemann E (1998) Legumes may be symbiont limited during old field succession. Amer Midl Natur 140:90–95
Lindstrom MJ, Schumacher TE, Blecha ML (1994) Management considerations for returning CRP lands to crop production. J Soil Water Conserv 49:420–425
Lupwayi NZ, Olsen PE, Sande ES, Keyser HH, Collins MM, Singleton PW, Rice WA (2000) Inoculant quality and its evaluation. Field Crops Res 65:259–270
Menge DNL, Pacala SW, Hedin LO (2009) Emergence and maintenance of nutrient limitation over multiple timescales in terrestrial ecosystems. Am Nat 173:164–175
Mueller-Dombois D, Ellenberg HNJ (2002) Aims and methods of vegetation ecology. Blackburn Press, Caldwell
Parkinson D, Paul EA (1982) Microbial biomass. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, Part 2. Chemical and microbiological properties. American Society of Agronomy, Madison, pp 821–826
Peres JRR, Suhet AR, Vargas MAT (1989) Estabelecimento de Bradyrhizobium japonicum num solo de cerrado pela inouclacao de sementes de arroz. Rev Bras Cienc Solo 13:35–39
Rademaker JLW, de Bruijn FJ (1997) Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer-assisted pattern analysis. In: Caetano-Anolles G, Gresshoff PM (eds) DNA markers: Protocols, applications and overviews. Wiley, New York, pp 151–171
Rastetter EB, Vitousek PM, Field C, Shaver GR, Herbert D, Agren GI (2001) Resource optimization and symbiotic nitrogen fixation. Ecosystems 4:369–388
Rowe HI (2010) Tricks of the trade: techniques and opinions from 38 experts in tallgrass prairie restoration. Restor Ecol 18:253–262
Samson F, Knopf F (1994) Prairie conservation in North America. Bioscience 44:418–421
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, New York
SAS Institute (2002) Statistical analysis system, Version 9.2. SAS Institute, Cary
Somasegaran P, Hoben HJ (1994) Handbook for rhizobia: Methods in legume-Rhizobium technology. Springer, New York
Spehn EM, Hector A, Joshi J et al (2005) Ecosystem effects of biodiversity manipulations in European grasslands. Ecol Monogr 75:37–63
Stephens JHG, Rask HM (2000) Inoculant production and formulation. Field Crop Res 65:249–258
Thies JE, Singleton PW, Bohlool BB (1991a) Influence of the size of indigenous rhizobial population on establishment and symbiotic performance of introduced rhizobia on field-grown legumes. Appl Environ Microbiol 57:19–28
Thies JE, Singleton PW, Bohlool BB (1991b) Modeling symbiotic performance of introduced rhizobia. Appl Environ Microbiol 57:29–37
Thrall PH, Millsom DA, Jeavons AC, Waayers M, Harvey GR, Bagnall DJ, Brockwell J (2005) Seed inoculation with effective root-nodule bacteria enhances revegetation success. J Appl Ecol 42:740–751
Tilman D (1987) Secondary succession and the pattern of plant dominance along experimental nitrogen gradients. Ecol Monogr 57:189–214
Tlusty B, Grossman JM, Graham PH (2004) Selection of rhizobia for prairie legumes used in restoration and reconstruction programs in Minnesota. Can J Microbiol 50:977–983
Tlusty B, van Berkum P, Graham PH (2005) Characteristics of the rhizobia associated with Dalea spp. in the Ordway, Kellogg-Weaver Dunes, and Hayden prairies. Can J Microbiol 51:15–23
van der Heijden MGA, Bakker R, Verwaal J, Scheublin TR, Rutten M, van Logtestijn R, Staehelin C (2006) Symbiotic bacteria as a determinant of plant community structure and plant productivity in dune grassland. FEMS Microbiol Ecol 56:178–187
Versalovic J, Schneider MF, de Bruijn J, Lupski JR (1994) Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Meth Mol Cell Biol 5:25–40
Villar-Salvador P, Valladares F, Dominguez-Lerena S, Ruiz-Diez B, Fernandez-Pascual M, Delgado A, Penuelas JL (2008) Functional traits related to seedling performance in the mediterranean leguminous shrub Retama sphaerocarpa: Insights from a provenance, fertilization, and rhizobial inoculation study. Environ Exp Bot 64:145–154
Vincent JM (1970) A manual for the practical study of the root-nodule bacteria. IBP Handbook No15. Blackwell Scientific Publisher, Oxford
Wilson SD, Gerry AK (1995) Strategies for mixed-grass prairie restoration: herbicide, tilling, and nitrogen manipulation. Restor Ecol 3:290–298
Zvomuya F, Rosen CJ, Russelle MP, Gupta SC (2003) Nitrate leaching and nitrogen recovery following application of polyolefin-coated urea to potato. J Environ Qual 32:480–489