Comparative Antioxidant Profiling of Tolerant and Sensitive Varieties of Brassica juncea L. to Arsenate and Arsenite Exposure
Tóm tắt
Comparative antioxidant profiling of tolerant (TPM-1) and sensitive (TM-4) variety of Brassica juncea L. was performed after exposure to arsenate [As(V)] and arsenite [As(III)]. TPM-1 demonstrated higher accumulation of As upon exposure to both 500 μM As(V) and 250 μM As(III) (49 and 37 μg g−1 dw after 15 days) as compared with that observed in TM-4. The activities of various antioxidant enzymes and the level of glutathione and proline demonstrated, in general, a comparatively better response in TPM-1 than in TM-4 that presumably allowed TPM-1 to tolerate higher As concentrations as compared with that of TM-4.
Tài liệu tham khảo
Ahsan N, Lee D-G, Alam I, Kim PJ, Lee JJ, Ahn Y-O, Kwak S-S, Lee I-J, Bahk JD, Kang KY, Renaut J, Komatsu S, Lee B-H (2008) Comparative proteomic study of arsenic-induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress. Proteomics 8:3561–3576
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
De Tullio MC, Gara LD, Paciolla C, Arrigoni O (1998) Dehydroascorbate-reducing proteins in maize are induced by the ascorbate biosynthesis inhibitor lycorine. Plant Physiol Biochem 36:433–440
Drążkiewicz M, Skórzyńska-Polit E, Krupa Z (2003) Response of ascorbate–glutathione cycle to excess copper in Arabidopsis thaliana (L.). Plant Sci 164:195–202
Foyer CH, Lelandais M, Kunert KJ (1994) Photooxidative stress in plants. Physiol Plant 92:696–717
Gupta M, Sharma P, Sarin NB, Sinha AK (2009) Differential response of arsenic stress in two varieties of Brassica juncea L. Chemosphere 74:1201–1208
Hartley-Whitaker J, Ainsworth G, Meharg AA (2001) Copper- and arsenate-induced oxidative stress in Holcus lanatus L. clones with differential sensitivity. Plant Cell Environ 24:713–722
Hemeda HM, Klein BP (1990) Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. J Food Sci 55:184–185, 192
Hissin PJ, Hilf R (1976) A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 74:214–226
Kaiser G, Kleiner O, Beisswenger C, Batschauer A (2009) Increased DNA repair in Arabidopsis plants overexpressing CPD photolyase. Planta 230:505–515
Khan I, Ahmad A, Iqbal M (2009) Modulation of antioxidant system for arsenic detoxification in Indian mustard. Ecotoxicol Environ Safety 72:626–634
Lee D, Schroeder J (2002) Phenotypic analysis of Arabidopsis mutants:heavy metal stress. In: Weigel D, Glazerbrook J (eds) Arabidopsis: a laboratory manual. Cold Spring Harbor Laboratory Press, New York, pp 75–77
Lowry OH, Rosenbrough NJ, Farr AL, Randal RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Mishra S, Srivastava S, Tripathi RD, Trivedi PK (2008) Thiol metabolism and antioxidant system complement each other during arsenate detoxification in Ceratophyllum demersum L. Aquat Toxicol 86:205–215
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
Noctor G, Foyer CH (1998) Ascorbate and glutathione. Keeping active oxygen under control. Annu Rev Plant Physiol Mol Biol 49:249–279
Norton GJ, Lou-Hing DE, Meharg AA, Price AH (2008) Rice–arsenate interactions in hydroponics: whole genome transcriptional analysis. J Exp Bot 59:2267–2276
Sharma SS, Dietz K-J (2009) The relationship between metal toxicity and cellular redox balance. Trends Plant Sci 14:43–50
Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5, 5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175:408–413
Srivastava S, Mishra S, Tripathi RD, Dwivedi S, Gupta DK (2006) Copper-induced oxidative stress and response of antioxidants and phytochelatins in Hydrilla verticillata (L.f.) Royle. Aquat Toxicol 80:405–415
Srivastava S, Mishra S, Tripathi RD, Dwivedi S, Trivedi PK, Tandon PK (2007) Phytochelatins and antioxidant systems respond differentially during arsenite and arsenate stress in Hydrilla verticillata (L.f.) Royle. Environ Sci Technol 41:2930–2936