Sodium-chloride transport in the thick ascending limb of Henle's loop
Tóm tắt
Isolated cells were prepared from the medullary thick ascending limb of Henle's loop (TALH) and the response of oxygen consumption was correlated with the active chloride transport system found in these cells. Oxygen consumption was 31.6 μl O2/mg protein·h and inhibited 50% by the absence of either sodium or chloride in the incubation medium. The absence of both sodium and chloride produced no further inhibition of oxygen consumption. Ouabain (10−4 M) inhibited oxygen consumption by 50% and the inhibitory effect depended on the presence of both sodium and chloride in the incubation medium. Further, furosemide inhibited oxygen consumption by a maximum of 50% at 10−3 M and also had no inhibitory effect if either sodium or chloride were absent. Furosemide had no effect on the Na, K-ATPase activity or ATP levels of the TALH cells. Thus, the data suggest that 50% of the oxygen consumption of the TALH cells is related to the movement of sodium and chloride into the cell and that the ions may be transported in a coupled manner. In addition the effect of various diuretics on oxygen consumption in the isolated TALH cells was tested. The diuretics could be grouped in three categories: (1) highly effective in inhibiting chloride-dependent oxygen consumption with an apparent inhibitory constant (K
i) of around 10−6 M, including the diuretics furosemide, bumetanide, ethacrynic acid-cysteine and piretanide, (2) diuretics which were less effective in inhibiting oxygen consumption with an apparentK
i of around 10−4 M, HOE 740 and ethacrynic acid, and (3) diuretics which were ineffective in inhibiting chloride-dependent oxygen consumption, amiloride and hydrochlorothiazide.
Tài liệu tham khảo
Baur H, Kasperek S, Pfaff E (1975) Criteria of viability of isolated liver cells. Hoppe-Seyler's Z Physiol Chem 356:827–838
Burg MB, Green N (1973) Function of the thick ascending limb of Henle's loop. Am J Physiol 224:659–668
Burg M, Stoner L, Cardinal J, Green N (1973) Furosemide effect on isolated perfused tubules. Am J Physiol 225:119–124
Burckhardt G, Kinne R, Stange G, Murer H (1980) The effects of potassium and membrane potential on sodium dependentl-glutamic acid uptake. Biochim Biophys Acta 599: 191–201
Cohen JJ, Kamm DE (1976) Renal metabolism: Relation to renal function. In: Brenner BM, Rector FC Jr (eds) The kidney, vol 1. W. B. Saunders, Philadelphia, pp. 126–214
Cunarro JA, Weiner MW (1978) Effects of ethacrynic acid and furosemide on respiration of isolated kidney tubules: the role of ion transport and the source of metabolic energy. J Pharmacol Exp Ther 206:198–206
Ebel H, Ehrich J, De Santo NG, Doerken U (1972) Plasma membranes of the kidney. III. Influence of diuretics on ATPase activity. Pflügers Arch 335:224–234
Eveloff J, Haase W, Kinne R (1980) Separation of renal medullary cells: Isolation of cells from the thick ascending limb of Henle's loop. J Cell Biol 87:672–681
Eveloff J, Kinne R, Kinne-Saffran E, Murer H, Silva P, Epstein FH, Stoff J, Kinter WB (1978) Coupled sodium and chloride transport into plasma membrane vesicles prepared from dogfish rectal gland. Pflügers Arch 378:87–92
Frizzell RA, Smith PL, Vosburgh E, Field M (1979) Coupled sodium-chloride influx across brush border of flounder intestine. J Memb Biol 46:27–39
Greger R (1980) The thick ascending limb of Henle's loop, a “leaky” nephron segment with rheogenic mechanisms at the luminal and antiluminal membrane. Pflügers Arch 384:R7
Grube G, Philipps K, Weiss C (1967) Die Wirkung von Änderung der Natrium-Konzentration im Suspensionsmedium und von Strophanthin auf die Sauerstoffaufnahme isolierter Nierenzellen der Ratte. Z Gesamte Exp Med 143:150–160
Heidrich H-G, Dew ME (1977) Homogenous cell populations from rabbit kidney cortex. J Cell Biol 74:780–788
Imai M (1977) Effect of bumetanide and furosemide on the thick ascending limb of Henle's loop of rabbits and rats perfused in vitro. Eur J Pharmacol 41:409–416
Kaissling B, Kriz W (1979) Structural analysis of the rabbit kidney. Advances in Anat Embryol Cell Biol 56:1–123
Kinne R (1978) Metabolic correlates of tubular transport. In: Giebisch G, Tosteson DC, Ussing HH (eds) Membrane transport in biology, IV, A and B. Chap II, Springer, Berlin Heidelberg New York, pp 529–562
Kinne R, Schmitz JE, Kinne-Saffran E (1971) The localization of the Na+−K+-ATPase in the cells of rat kidney cortex. A study on isolated plasma membranes. Pflügers Arch 329:191–206
Klahr S, Yates J, Bourgoigne J (1971) Inhibition of glycolysis by ethacrynic acid and furosemide. Am J Physiol 221:1038–1043
Lassen UV, Thaysen JH (1961) Correlation between sodium transport and oxygen consumption in isolated renal tissue. Biochim Biophys Acta 47:616–618
Lowry OH, Rosebrough NT, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Manuel MA, Weiner MW (1976) Effects of ethacrynic acid and furosemide on isolated rat kidney mitochondria: inhibition of electron transport in the region of phosphorylation site II. J Pharmacol Exp Ther 198:209–221
McManus TJ, Schmidt III WF (1978) Ion and co-ion transport in avian red cells. In: Hoffman JF (ed) Membrane transport processes, vol 1. Raven Press, New York, pp 79–106
Rocha AS, Kokko JP (1973) Sodium chloride and water transport in the medullary thick ascending limb of Henle. Evidence for active chloride transport. J Clin Invest 52:612–623
Schmidt U, Dubach UC (1970) The behaviour of Na+ K+-activated adenosine triphosphatase in various structures of the rat nephron after furosemide application. Nephron 7:447–458
Shaver JL, Stirling C (1978) Ouabain binding to renal tubules of the rabbit. J Cell Biol 76:278–292
Solomon RJ, Silva P, Stevens A, Epstein J, Stoff JS, Spokes K, Epstein FH (1977) Mechanism of chloride transport in the rectal gland ofSqualus acanthias. Bull Mt Desert Island Biol Lab 17:59–63
Thurau K (1961) Renal Na-reabsorption and O2-uptake in dogs during hypoxia and hydrochlorothiazide infusion. Proc Soc Exp Biol Med 106:714–717
Ullrich KJ (1958) Aktiver Natriumstransport und Sauerstoffverbrauch in der äußeren Markzone der Niere. Pflügers Arch 267:207–217