Green Fluorescent Protein as a Marker for Gene Expression

American Association for the Advancement of Science (AAAS) - Tập 263 Số 5148 - Trang 802-805 - 1994
Martin Chalfie1, Yuan Tu1, Ghia Euskirchen1, William W. Ward2, Douglas C. Prasher3
1Department of Biological Sciences, Columbia University, New York, NY 10027
2Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, NJ 08903, USA
3Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.

Tóm tắt

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic ( Escherichia coli ) or eukaryotic ( Caenorhabditis elegans ) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.

Từ khóa


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