Characterization of an antibacterial substance produced by Pediococcus pentosaceus Iz3. 13 isolated from Japanese fermented marine food
Tóm tắt
Fermentation of raw marine food is popular in Japan, but is occasionally associated with Clostridium botulinum outbreaks. Lactic acid bacteria in fermented food produce antibacterial substances (ABS). An ABS producing strain Iz. 3. 13 was isolated from commercially fermented ‘izushi’. The purpose of this study was to characterize strain Iz. 3. 13 and the ABS it produced. Strain Iz. 3. 13 was identified as Pediococcus pentosaceus by morphological, biochemical characteristics and 16S rDNA sequence similarity. Strain Iz. 3. 13ABS produced and ABS inhibited Listeria and Cl. botulinum. The ABS was inhibited by α-chymotrypsin and proteinase K, but not by catalase, lipase, or α-amylase indicating the ABS was a bacteriocin. The bacteriocin remained active at 100°C for 15 min, and pH 2–8. Mode of action of the bacteriocin against Listeria monocytogenes was bactericidal and bacteriolytic. Partial analysis of the purified bacteriocin by Edman degradation, showed a 22-amino acid residue closely related to Pediocin AcH. However, MALDI-TOF-MS analysis estimated a molecular mass of 4621.6 Da. This is a first report of a Cl. botulinum-inhibiting bacteriocin-producer from traditional Japanese fermented marine food. The bacteriocin produced by Ped. pentosaceus Iz. 3. 13 might have potential to ensure the safety of fermented marine food.
Tài liệu tham khảo
Tanikawa E. Marine Products in Japan. Koseisha Koseikaku Co. Ltd, Tokyo. 1985.
Gilliland SE. Role of starter culture bacteria in food preservation. In: Gilliland SE (ed.). Bacteria Starter Cultures for Foods. CRC Press, Boca Raton, FL. 1985; 176–185.
De Vuyst L, Vandamme EJ. Bacteriocins of Lactic Acid Bacteria: Microbiology, Genetics and Applications. Chapman & Hall, London. 1994.
Naidu AS, Clemens RA. Probiotics. In: Naidu AS (ed.). Natural Food Antimicrobial Systems. CRC Press, Boca Raton, FL. 2000; 431–462.
Ross PP, Morgan S, Hill C. Preservation and fermentation: past, present and future. Int. J. Food Microbiol. 2002; 79: 3–16.
Nakamura Y, Iida H, Saheki K. Food poisoning by Cl. Botulinum at Iwanai district in Hokkaido. Report of the Hokkaido Institute of Public Health. Special Report. 1952.
Tagg JR, Dajani AS, Wannamaker LW. Bacteriocins of Gram-positive bacteria. Bacteriol. Rev. 1976; 40: 722–756.
Hoover DG, Walsh PM, Kolaetis KM, Daly MM. A bacteriocin produced by Pediococcus pentosaceus associated with a 5.5 mega Dalton plasmid. J. Food Prot. 1988; 51: 29–31.
Carminati D, Giraffa G, Bossi MG. Bacteriocin-like inhibitors of Streptococcus lactis against Listeria monocytogenes. J. Food Prot. 1989; 52: 614–617.
Harris LJ, Daeschel MA, Stiles ME, Klaenhammer TR. Antimicrobial activity of lactic acid bacteria against Listeria monocytogenes. J. Food Prot. 1989; 52: 384–387.
Schillinger U, Lucke FK. Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 1989; 55: 1901–1906.
Debevere JM. The use of buffered acidulant systems to improve the microbiological stability of acid foods. Food Microbiol. 1987; 4: 105–114.
Yamazaki K, Suzuki M, Kawai Y, Inoue N, Monteville TJ. Inhibition of Listeria monocytogenes in cold smoked salmon by Carnobacterium piscicola CS526 isolated from frozen Surimi. J. Food Prot. 2003; 66: 1420–1425.
Kraemer EO, Stamm AJ. Mohr’s method for the determination and of silver and halogens in other than neutral solutions. J. Am. Chem. Soc. 1924; 46: 2707–2709.
Wikownski K, Montville TJ. Use of meat isolated Lactobacillus bavaricus MN, to inhibit Listeria monocytogenes growth in a model meat gravy system. J. Food Saf. 1992; 13: 19–31.
Harrigan WF. Laboratory Methods in Food Microbiology. Academic Press, London. 1998.
Mori K, Yamazaki K, Ishiyama T, Katsumata M, Kobayashi K, Kawai Y, Inoue N, Shinano H. Comparative sequence analyses of the genes coding for 16S rRNA of Lactobacillus casei related taxa. Int. J. Syst. Bacteriol. 1997; 47: 54–57.
Altschul SF, Madden TL, Schaffer AA, Zhang JZZ, Miller W, Lipman DJ. Gapped BLAST and PSI-Blast: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25: 3389–3402.
Yang R, Johnson MC, Ray B. Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 1992; 58: 3355–3359.
Schagger H, Von Jagow G. Tricine-sodiumdodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range of 1–100 kDa. Anal. Biochem. 1987; 166: 368–379.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–685.
Gálvez A, Valdivia E, Abriouel H, Camafeita E, Mendez E, Martinez-Bueno M, Maqueda M. Isolation and characterization of enterocin EJ97, a bacteriocin produced by Enterococcus faecalis EJ97. Arch. Microbiol. 1998; 171: 59–65.
Garvie EJ. Genus Pediococcus. In: Sneath PHA, Hold JG, (eds). Bergeys’s Manual of Systematic Bacteriology Williams & Wilkins Co Baltimore. 1986: 1075–1079.
Motlagh AM, Bhunia AK, Szostek F, Hansen T, Johnson MC, Ray B. Nucleotide and amino acid sequence of pap-gene (pediocin AcH production) in Pediococcus acidilactici H. Lett. Appl. Microbiol. 1992; 15: 45–48.
Sasaki M, Kawai Y, Yoshimizu M, Shinano H. Changes in Chemical composition and microbial flora of salmon Izushi during ripening process. Nippon Suisan Gakkaishi 2004; 70: 928–937.
Fujii T, Saito N, Ishitani T, Okuzumi M. Presence of antibiotic-producing streptococci in squid sauce during shiokara fermentation. Lett. Appl. Microbiol. 1992; 14: 115–117.
Okuda I. Chemical studies on the sauced fermented visceral mass of bonito (Katsuo no shiokara). J. Sci. Agric. Soc. 1912; 115: 11.
Tanikawa E, Matsuura H. A study on the manufacture of ‘shottusuru’ from salmon viscera as a raw material. Nippon Suisan Gakkaishi 1944; 12: 187–191.
Lozano JC, Meyer JN, Sletten K, Pelaz K, Nes IF. Purification and amino acid sequence of a bacteriocin produced by Pediococcus acidilactici. J. Gen. Microbiol. 1992; 138: 1985–1990.
Gonzalez CF, Kunka BS. Plasmid-associated bacteriocin production and sucrose fermentation in Pediococcus acidilactici. Appl. Environ. Microbiol. 1987; 53: 2534–2538.
Bhunia AK, Johnson MC, Ray B, Kalachayanand N. Mode of action of pediocin AcH from Pedicoccus acidilactici H on sensitive bacteria strains. J. Appl. Bacteriol. 1991; 70: 25–30.
Motlagh AM, Holla S, Johnson MC, Ray B, Field RA. Inhibiton of Listeria spp. in sterile food systems by pediocin AcH, a bacteriocin from Pediococcus acidilactici H. J. Food Prot. 1992; 55: 337–343.
Daeschel MA, Klaenhammer TR. Association of a 13.6 megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity. Appl. Environ. Microbiol. 1985; 50: 1538–1541.
Bhunia AK, Johnson MC, Ray B. Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pedicoccus acidilactici. J. Appl. Bacteriol. 1988; 65: 261–268.
Ray B. Pediocins of Pediococcus acidilactici as food preservatives. In: Ray B, Daeschel MA (eds). Food Preservatives of Microbial Origin. CRC Press, Boca Raton, FL. 1992; 265–322.
Spelhuag SR, Harlander SK. Inhibition of food borne bacterial pathogens by bacteriocins from Lactococcus lactis and Pediococcus pentosaceus. J. Food Prot. 1989; 52: 856–862.
Chikindas ML, Monteville TJ. Perspectives for application of bacteriocins as food preservatives. In: Juneja VK, Sofos JN (eds). Control. of Food Borne Microorganisms. Marcel Dekker, New York, 2002; 303–321.
Drider D, Fimland G, Hechard Y, McMullen LM, Prevost H. The continuing story of class IIa bacteriocins. Microbiol. Mol. Biol. Rev. 2006; 70: 564–582.