Homologous growth hormone (GH) binding in gilthead sea bream (<i>Sparus aurata</i>). Effect of fasting and refeeding on hepatic GH‐binding and plasma somatomedin‐like immunoreactivity

Journal of Fish Biology - Tập 44 Số 2 - Trang 287-301 - 1994
Jaume Pérez‐Sánchez1, Hilario Martí-Palanca1, Le Bail Py2
1Instituto de Acuicultura de Torre de la Sal, CSIC, 12595 Torre de la Sal, Ribera de Cabanes, Castellon, Spain
2Laboraloire de Physiologie des Poissons, INRA, Campus de Beaulieu, 35042 Rennes Cedex, France

Tóm tắt

Specific binding of gilthead sea bream growth hormone (sbGH) to liver membrane preparations was a time and temperature dependent process, and was saturable by increasing amounts of membrane proteins. Scatchard analysis evidenced a single class of high‐affinity and lowcapacity binding sites. Ovine prolactin, recombinant tilapia prolactin, carp gonadotropin and chinook salmon gonadotropin did not compete for the125I‐sbGH binding sites, while recombinant trout GH, bovine GH and human GH displaced iodinated sbGH in a dose dependent‐manner. IGF‐I‐like immunoreactivity was detected after acidification of plasma and removal of IGF‐I binding activity. A parallel displacement to the rhIGF‐1 standard was observed with extracted plasma samples. Free and total hepatic GH‐binding decreased during long‐term starvation (3–9 weeks), returning to control values during the refeeding period. Plasma IGF‐I‐like immunoreactivity showed a similar trend. To our knowledge, this is the first report that indicates a coordinated regulation of GH‐binding and plasma somatomedin‐like activity in a typical marine fish.

Từ khóa


Tài liệu tham khảo

10.1210/jcem-67-2-265

Baxter R. C., 1988, The insulin‐like growth factors and their binding proteins, Comparative Biochemistry and Physiology, 91, 229

10.1210/mend-3-12-2005

Cuatrecasas P., 1977, Hormone receptor complexes and their modulation of membrane function, Recent Progress in Hormone Research, 31, 37

10.1016/0016-6480(85)90384-3

Delahunty G., 1992, Insulin‐like growth factor I and insulin‐like growth factor‐binding protein in the channel catfish (Ictalums punctatus)

10.1016/0016-6480(89)90210-4

10.1002/jez.1402560316

10.1677/joe.0.1330211

10.1016/0016-6480(90)90117-5

10.1016/0016-6480(79)90217-X

10.1677/joe.0.120R019

10.1210/jcem-51-1-12

10.1172/JCI108816

10.1016/0303-7207(79)90072-8

10.1677/joe.0.1310057

10.1016/0016-6480(92)90256-J

10.1002/jez.1402560308

10.1042/bj0890114

10.1016/0016-6480(91)90165-3

10.1677/joe.0.1220611

10.1210/endo-105-2-414

10.1002/jez.1402630213

10.1210/endo-104-6-1631

10.1016/0016-6480(78)90047-3

10.1007/BF00004713

10.1139/z93-211

10.3181/00379727-184-42473

10.1002/9780470719831.ch2

10.1016/0044-8486(85)90268-6

10.1677/joe.0.0970243

10.1210/endo-122-4-1291

10.1002/jez.1402370209

Martal J., 1972, Determination radioimmunologique des hormones de croissance ovine et obine: étude des conditions ?iodation et obtention ?une hormone somatotrope radiodée sans prolactine contaminante, Comptes Rendues àľAcademie des Sciences de Paris, 274, 2893

10.1002/jez.1402620206

10.1016/0024-3205(88)90229-9

10.1016/0016-6480(92)90083-V

10.1016/0016-6480(92)90132-4

Ng T. B., 1991, Insulin‐like growth factor I‐like immunoreactivity in serum and tissues of the tilapia, Oreochromis mossambicus, Biochemistry International, 24, 359

Nissley S. P., 1985, Hormonal Proteins and Peptides, 127

10.1002/jez.1402650604

10.1007/BF00004588

10.1002/jez.1402620308

10.1677/joe.0.1300425

Salmon W. D., 1957, A hormonally controlled serum factor which stimulates sulfate incorporation by cartilage in vitro, Journal of Laboratory and Clinical Medicine, 49, 825

10.1210/mend-4-1-91

10.1016/0016-6480(85)90290-4

10.1677/joe.0.0950059

10.1016/0016-6480(91)90126-Q

Zapf J., 1980, Serum levels of insulin‐like growth factor (IGF) and its carrier protein in various metabolic disorders, Act a Endocrinologica, 95, 505

Thông tin
Thông tin xuất bản

Journal of Fish Biology

Tập 44 Số 2

287-301

Thông tin tác giả