Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes

Genes and Development - Tập 23 Số 8 - Trang 939-950 - 2009
Jon Reinders1, Brande B. H. Wulff2, Marie Mirouze1, Arturo Marí‐Ordóñez2, Mélanie Dapp1, Wilfried Rozhon3, Etienne Bucher1, Grégory Theiler1, Jerzy Paszkowski1
1Department of Plant Biology, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland
2Instituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, 46022 Valencia, Spain
3Max F. Perutz Laboratories, University of Vienna, 1030 Vienna, Austria

Tóm tắt

Transgenerational epigenetic inheritance has been defined by the study of relatively few loci. We examined a population of recombinant inbred lines with epigenetically mosaic chromosomes consisting of wild-type and CG methylation-depleted segments (epiRILs). Surprisingly, transposons that were immobile in the parental lines displayed stochastic movement in 28% of the epiRILs. Although analysis after eight generations of inbreeding, supported by genome-wide DNA methylation profiling, identified recombined parental chromosomal segments, these were interspersed with unexpectedly high frequencies of nonparental methylation polymorphism. Hence, epigenetic inheritance in hybrids derived from parents with divergent epigenomes permits long-lasting epi-allelic interactions that violate Mendelian expectations. Such persistently “unstable” epigenetic states complicate linkage-based epigenomic mapping. Thus, future epigenomic analyses should consider possible genetic instabilities and alternative mapping strategies.

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Genes and Development

Tập 23 Số 8

939-950

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