Genome‐wide analysis of DNA methylation in relation to socioeconomic status during development and early adulthood

Wiley - Tập 169 Số 1 - Trang 3-11 - 2019
Thomas W. McDade1,2,3, Calen P. Ryan2, Meaghan J. Jones4,5,6, Morgan K. Hoke7,8, Judith Rafaelita Borja9,10, Gregory E. Miller11,3, Christopher W. Kuzawa2,3, Michael S. Kobor4,1,6
1Child and Brain Development Program, Canadian Institute for Advanced Research, Toronto, Ontario, Canada
2Department of Anthropology, Northwestern University, Evanston, Illinois
3Institute for Policy Research, Northwestern University, Evanston, Illinois
4BC Children's Hospital Research Institute
5Department of Biochemistry and Medical Genetics, University of Manitoba
6Department of Medical Genetics, University of British Columbia
7Department of Anthropology, University of Pennsylvania, Philadelphia, Pennsylvania
8Population Studies Center, University of Pennsylvania, Philadelphia, Pennsylvania
9Department of Nutrition and Dietetics, University of San Carlos, Cebu City, Philippines
10USC-Office of Population Studies Foundation, Inc., University of San Carlos, Cebu City, Philippines
11Department of Psychology, Northwestern University, Evanston, Illinois

Tóm tắt

AbstractObjectives

Socioeconomic status (SES) is a powerful determinant of health, but the underlying biological mechanisms are poorly understood. This study investigates whether levels of DNA methylation at CpG sites across the genome are associated with SES in a cohort of young adults in the Philippines.

Methods

DNA methylation was assayed with the Illumina HumanMethylation450 Bead Chip, in leukocytes from 489 participants in the Cebu Longitudinal Health and Nutrition Survey (mean age = 20.9 years). SES was measured in infancy/childhood and adulthood, and was based on composite measures of income, assets, and education. Genome‐wide analysis of variable probes identified CpG sites significantly associated with SES after adjustment for multiple comparisons. Functional enrichment analysis was used to identify biological pathways associated with these sites.

Results

A total of 2,546 CpG sites, across 1,537 annotated genes, were differentially methylated in association with SES. In comparison with high SES, low SES was associated with increased methylation at 1,777 sites, and decreased methylation at 769 sites. Functional enrichment analysis identified over‐representation of biological pathways related to immune function, skeletal development, and development of the nervous system.

Conclusions

Socioeconomic status predicts DNA methylation at a large number of CpG sites across the genome. The scope of these associations is commensurate with the wide range of biological systems and health outcomes that are shaped by SES, and these findings suggest that DNA methylation may play an important role.

Từ khóa


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