The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses

Genes and Development - Tập 11 Số 23 - Trang 3194-3205 - 1997
Jinrong Peng1, Pierre Carol2, Donald E. Richards1, Kathryn E. King1, Rachel J. Cowling3, George P. Murphy1, Nicholas P. Harberd1
1Department of Molecular Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UJ, UK.
2Laboratoire de Ge ´ne ´tique Mole ´culaire Ve ´ge ´tale, Uni-versite ´Joseph Fourier, 38041 Grenoble CEDEX, France;
3Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles CEDEX, France.

Tóm tắt

The Arabidopsis gai mutant allele confers a reduction in gibberellin (GA) responsiveness. Here we report the molecular cloning of GAI and a closely related gene GRS. The predicted GAI (wild-type) and gai (mutant) proteins differ only by the deletion of a 17-amino-acid segment from within the amino-terminal region. GAI and GRS contain nuclear localization signals, a region of homology to a putative transcription factor, and motifs characteristic of transcriptional coactivators. Genetic analysis indicates that GAI is a repressor of GA responses, that GA can release this repression, and that gai is a mutant repressor that is relatively resistant to the effects of GA. Mutations at SPY and GAR2 suppress thegai phenotype, indicating the involvement of GAI, SPY, and GAR2 in a signaling pathway that regulates GA responses negatively. The existence of this pathway suggests that GA modulates plant growth through derepression rather than through simple stimulation.

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Genes and Development

Tập 11 Số 23

3194-3205

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