Diversity of coronavirus in bats from Eastern Thailand
Tóm tắt
Bats are reservoirs for a diverse range of coronaviruses (CoVs), including those closely related to human pathogens such as Severe Acute Respiratory Syndrome (SARS) CoV and Middle East Respiratory Syndrome CoV. There are approximately 139 bat species reported to date in Thailand, of which two are endemic species. Due to the zoonotic potential of CoVs, standardized surveillance efforts to characterize viral diversity in wildlife are imperative. A total of 626 bats from 19 different bat species were individually sampled from 5 provinces in Eastern Thailand between 2008 and 2013 (84 fecal and 542 rectal swabs). Samples collected (either fresh feces or rectal swabs) were placed directly into RNA stabilization reagent, transported on ice within 24 hours and preserved at −80°C until further analysis. CoV RNA was detected in 47 specimens (7.6%), from 13 different bat species, using broadly reactive consensus PCR primers targeting the RNA-Dependent RNA Polymerase gene designed to detect all CoVs. Thirty seven alphacoronaviruses, nine lineage D betacoronaviruses, and one lineage B betacoronavirus (SARS-CoV related) were identified. Six new bat CoV reservoirs were identified in our study, namely Cynopterus sphinx, Taphozous melanopogon, Hipposideros lekaguli, Rhinolophus shameli, Scotophilus heathii and Megaderma lyra. CoVs from the same genetic lineage were found in different bat species roosting in similar or different locations. These data suggest that bat CoV lineages are not strictly concordant with their hosts. Our phylogenetic data indicates high diversity and a complex ecology of CoVs in bats sampled from specific areas in eastern regions of Thailand. Further characterization of additional CoV genes may be useful to better describe the CoV divergence.
Tài liệu tham khảo
citation_journal_title=Proc Natl Acad Sci U S A; citation_title=Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats; citation_author=SK Lau, PC Woo, KS Li, Y Huang, HW Tsoi, BH Wong; citation_volume=36; citation_publication_date=2005; citation_pages=14040-5; citation_doi=10.1073/pnas.0506735102; citation_id=CR1
citation_journal_title=J Virol; citation_title=Coexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup; citation_author=SK Lau, RW Poon, BH Wong, M Wang, Y Huang, H Xu; citation_volume=84; citation_publication_date=2010; citation_pages=11385-94; citation_doi=10.1128/JVI.01121-10; citation_id=CR2
citation_journal_title=Science; citation_title=Bats are natural reservoirs of SARS-like coronaviruses; citation_author=W Li, Z Shi, M Yu, W Ren, C Smith, JH Epstein; citation_volume=310; citation_publication_date=2005; citation_pages=676-9; citation_doi=10.1126/science.1118391; citation_id=CR3
citation_journal_title=J Virol; citation_title=Prevalence and genetic diversity of coronaviruses in bats from China; citation_author=XC Tang, JX Zhang, SY Zhang, P Wang, XH Fan, LF Li; citation_volume=80; citation_publication_date=2006; citation_pages=7481-90; citation_doi=10.1128/JVI.00697-06; citation_id=CR4
citation_journal_title=Virology; citation_title=Molecular diversity of coronaviruses in bats; citation_author=PC Woo, SK Lau, KS Li, RW Poon, BH Wong, HW Tsoi; citation_volume=351; citation_publication_date=2006; citation_pages=180-7; citation_doi=10.1016/j.virol.2006.02.041; citation_id=CR5
citation_journal_title=Emerg Infect Dis; citation_title=Middle East respiratory syndrome coronavirus in bats Saudi Arabia; citation_author=ZA Memish, N Mishra, KJ Olival, SF Fagbo, V Kapoor, JH Epstein; citation_volume=19; citation_publication_date=2013; citation_pages=1819-23; citation_doi=10.3201/eid1911.131172; citation_id=CR6
citation_journal_title=Emerg Infect Dis; citation_title=Human betacoronavirus 2c EMC/2012-related viruses in bats Ghana and Europe; citation_author=A Annan, HJ Baldwin, VM Corman, SM Klose, M Owusu, EE Nkrumah; citation_volume=9; citation_publication_date=2013; citation_pages=456-9; citation_doi=10.3201/eid1903.121503; citation_id=CR7
citation_journal_title=J Virol; citation_title=Characterization of a novel betacoronavirus related to middle East respiratory syndrome coronavirus in European hedgehogs; citation_author=VM Corman, R Kallies, H Philipps, G Göpner, MA Müller, I Eckerle; citation_volume=88; citation_publication_date=2014; citation_pages=717-24; citation_doi=10.1128/JVI.01600-13; citation_id=CR8
citation_journal_title=Virus Genes; citation_title=Alpha and lineage C betaCoV infections in Italian bats; citation_author=P Benedictis, S Marciano, D Scaravelli, P Priori, B Zecchin, I Capua; citation_volume=48; citation_publication_date=2014; citation_pages=366-71; citation_doi=10.1007/s11262-013-1008-x; citation_id=CR9
citation_journal_title=Emerg Infect Dis; citation_title=Close relative of human Middle East respiratory syndrome coronavirus in bat, South Africa; citation_author=NL Ithete, S Stoffberg, VM Corman, VM Cottontail, LR Richards, MC Schoeman; citation_volume=19; citation_publication_date=2013; citation_pages=1697-9; citation_doi=10.3201/eid1910.130946; citation_id=CR10
citation_journal_title=Emerg Infect Dis; citation_title=Group C betacoronavirus in bat guano fertilizer, Thailand; citation_author=S Wacharapluesadee, C Sintunawa, T Kaewpom, K Khongnomnan, KJ Olival, JH Epstein; citation_volume=19; citation_publication_date=2013; citation_pages=1349-51; citation_doi=10.3201/eid1908.130119; citation_id=CR11
citation_journal_title=J Virol; citation_title=Discovery of seven novel Mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus; citation_author=PC Woo, SK Lau, CS Lam, CC Lau, AK Tsang, JH Lau; citation_volume=86; citation_publication_date=2012; citation_pages=3995-4008; citation_doi=10.1128/JVI.06540-11; citation_id=CR12
citation_journal_title=J Wildlife Thailand; citation_title=A Checklist of Bats (Mammalia:Chiroptera) in Thailand; citation_author=D Soisook; citation_volume=18; citation_publication_date=2011; citation_pages=121-51; citation_id=CR13
citation_journal_title=Zootaxa; citation_title=A new species of Murina (Mammalia: Chiroptera: Vespertilionidae) from peninsular Thailand; citation_author=P Soisook, S Karapan, C Satasook, PJ Bates; citation_volume=3746; citation_publication_date=2013; citation_pages=567-79; citation_doi=10.11646/zootaxa.3746.4.4; citation_id=CR14
citation_journal_title=Infect Genet Evol; citation_title=SARS-Coronavirus ancestor’s foot-prints in South-East Asian bat colonies and the refuge theory; citation_author=MA Gouilh, SJ Puechmaille, JP Gonzalez, E Teeling, P Kittayapong, JC Manuguerra; citation_volume=11; citation_publication_date=2011; citation_pages=1690-702; citation_doi=10.1016/j.meegid.2011.06.021; citation_id=CR15
citation_title=Mammals of Thailand; citation_publication_date=1988; citation_id=CR16; citation_author=B Lekagul; citation_author=JA McNeely; citation_publisher=Bangkok
citation_title=A field guide to the mammals of Thailand and south-east Asia; citation_publication_date=2008; citation_id=CR17; citation_author=CM Francis; citation_publisher=New Holland Publishers (UK) Ltd
citation_journal_title=Emerg Infect Dis; citation_title=Bat coronaviruses and experimental infection of bats, the Philippines; citation_author=S Watanabe, JS Masangkay, N Nagata, S Morikawa, T Mizutani, S Fukushi; citation_volume=16; citation_publication_date=2010; citation_pages=1217-23; citation_doi=10.3201/eid1608.100208; citation_id=CR18
citation_journal_title=J Gen Virol; citation_title=Coronaviruses in bent-winged bats (Miniopterus spp.); citation_author=DK Chu, LL Poon, KH Chan, H Chen, Y Guan, KY Yuen; citation_volume=87; citation_publication_date=2006; citation_pages=2461-6; citation_doi=10.1099/vir.0.82203-0; citation_id=CR19
citation_journal_title=J Virol; citation_title=Genomic characterization of severe acute respiratory syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences; citation_author=JF Drexler, F Gloza-Rausch, J Glende, VM Corman, D Muth, M Goettsche; citation_volume=84; citation_publication_date=2010; citation_pages=11336-49; citation_doi=10.1128/JVI.00650-10; citation_id=CR20
citation_journal_title=Virus Res; citation_title=Genomic characterization of seven distinct bat coronaviruses in Kenya; citation_author=Y Tao, K Tang, M Shi, C Conrardy, KS Li, SK Lau; citation_volume=167; citation_publication_date=2012; citation_pages=67-73; citation_doi=10.1016/j.virusres.2012.04.007; citation_id=CR21
citation_journal_title=Virology; citation_title=Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome; citation_author=SK Lau, PC Woo, KS Li, Y Huang, M Wang, CS Lam; citation_volume=367; citation_publication_date=2007; citation_pages=428-39; citation_doi=10.1016/j.virol.2007.06.009; citation_id=CR22
citation_journal_title=J Gen Virol; citation_title=Coronaviruses in bats from Mexico; citation_author=SJ Anthony, R Ojeda-Flores, O Rico-Chávez, I Navarrete-Macias, CM Zambrana-Torrelio, MK Rostal; citation_volume=94; citation_publication_date=2013; citation_pages=1028-38; citation_doi=10.1099/vir.0.049759-0; citation_id=CR23
citation_journal_title=PLoS Pathog; citation_title=Quasispecies theory and the behavior of RNA viruses; citation_author=AS Lauring, R Andino; citation_volume=6; citation_publication_date=2010; citation_pages=e1001005; citation_doi=10.1371/journal.ppat.1001005; citation_id=CR24
citation_journal_title=Arch Virol; citation_title=Detection of alpha and betacoronaviruses in multiple Iberian bat species; citation_author=A Falcón, S Vázquez-Morón, I Casas, C Aznar, G Ruiz, F Pozo; citation_volume=156; citation_publication_date=2011; citation_pages=1883-90; citation_doi=10.1007/s00705-011-1057-1; citation_id=CR25
citation_journal_title=Emerg Infect Dis; citation_title=Detection and phylogenetic analysis of group 1 coronaviruses in South American bats; citation_author=CV Carrington, JE Foster, HC Zhu, JX Zhang, GJ Smith, N Thompson; citation_volume=14; citation_publication_date=2008; citation_pages=1890-3; citation_doi=10.3201/eid1412.080642; citation_id=CR26
citation_journal_title=J Virol; citation_title=Recent transmission of a novel alphacoronavirus, bat coronavirus HKU10, from Leschenault’s rousettes to pomona leaf-nosed bats: first evidence of interspecies transmission of coronavirus between bats of different suborders; citation_author=SK Lau, KS Li, AK Tsang, CT Shek, M Wang, GK Choi; citation_volume=86; citation_publication_date=2012; citation_pages=11906-18; citation_doi=10.1128/JVI.01305-12; citation_id=CR27
citation_journal_title=J Gen Virol; citation_title=Intraspecies diversity of SARS-like coronaviruses in Rhinolophus sinicus and its implications for the origin of SARS coronaviruses in humans; citation_author=J Yuan, CC Hon, Y Li, D Wang, G Xu, H Zhang; citation_volume=91; citation_publication_date=2010; citation_pages=1058-62; citation_doi=10.1099/vir.0.016378-0; citation_id=CR28
citation_journal_title=J Gen Virol; citation_title=Genomic characterizations of bat coronaviruses (1A, 1B and HKU8) and evidence for co-infections in Miniopterus bats; citation_author=DK Chu, JS Peiris, H Chen, Y Guan, LL Poon; citation_volume=89; citation_publication_date=2008; citation_pages=1282-7; citation_doi=10.1099/vir.0.83605-0; citation_id=CR29