The medical diagnostic approaches with phylogenetic analysis for rare Brucella spp. diagnosis in Taiwan

BioMedicine - Tập 5 - Trang 1-7 - 2015
Ni Tien1,2, Yun-Ju Sung1, Yi-Chih Chang2, Bang-Jau You3, Michelle Liang4, Yun-Ping Lim5, Wen-Yu Ho6, Hsiu-Shen Lin1, Mao-Wang Ho7, Chen-Mao Ho1,7, Chao-Chin Chang8, Yu-Ching Lan9
1Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan
2Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan
3Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
4Department of Microbiology, UCLA, Los Angeles, USA
5Department of Pharmacy, College of Pharmacy, China Medical University, Taichung, Taiwan
6Department of Laboratory Medicine, China Medical University Beigang Hospital, Taichung, Taiwan
7Department of Internal Medicine, School of Medicine, China Medical University, Taichung, Taiwan
8Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung, Taiwan
9Department of Health Risk Management, China Medical University, Taichung, Taiwan

Tóm tắt

Brucellosis is a bacterial zoonotic disease which can be easy to misdiagnose in clinical microbiology laboratories. In the present study, we have tried to improve the current clinical method for detecting Brucella spp. and its antibiotic characteristics. Our method begins with detecting the clinical isolate through traditional biochemical methods and automatic identification systems. Then, we move on to editing the sequence for BLAST allows us to compare 16s rRNA sequences with sequences from other species, allowing the gene level to be determined. Next, the phylogenetic analysis of multiple genetic loci is able to determine the evolutionary relationships between our bacteria strain and those from other locations. Finally, an anti-microbial susceptibility test hones in on the level of antibacterial activity that the bacteria displays. Employing these four steps in concert is extremely effective in identifying rare bacteria. Thus, when attempting to determine the identity of rare bacteria such as Brucella, utilizing these four steps from our research should be highly effective and ultimately prevent further identification errors and misdiagnoses. The standards we have suggested to identify rare bacteria strains is applicable not only to Brucella, but also to other rarely encountered bacteria.

Tài liệu tham khảo

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