CD44 Is Associated with the Aggressive Phenotype of Nasopharyngeal Carcinoma through Redox Regulation

International Journal of Molecular Sciences - Tập 14 Số 7 - Trang 13266-13281
Chien‐Hung Lin1,2, Peir‐Haur Hung3, Yann‐Jang Chen4,5,2
1Department of Pediatrics, Zhongxing Branch, Taipei City Hospital, Taipei 103, Taiwan
2Institute of Clinical Medicine, National Yang-Ming University, Taipei, 112, Taiwan
3Department of Medical Research, Ditmanson Medical Foundation Chia-yi Christian Hospital, Chia-yi 600, Taiwan
4Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei 112, Taiwan
5Department of Pediatrics, Renai Branch, Taipei City Hospital, Taipei 106, Taiwan

Tóm tắt

Recent studies have shown that cancer stem-like cells (CSCs) within a tumor have the capacity for self-renewal and differentiation, and are associated with an aggressive phenotype and therapeutic resistance. Studies have also associated tumor progression with alterations in the levels of intracellular reactive oxygen species (ROS). In this study, we cultured nasopharyngeal carcinoma (NPC) CSCs in conditions that allowed sphere formation. The resulting sphere cells displayed stemness properties, characteristics of the epithelial–mesenchymal transition (EMT), and increased expression of the CSC surface marker CD44. We further evaluated the association between CD44 expression and EMT marker expression, and any correlation with redox status, in these CSCs. We showed that the EMT in sphere cells is associated with the upregulation of CD44 expression and increased ROS generation, which might promote NPC aggressiveness. We also identified the coexpression of CD44 with the EMT marker N-cadherin in sphere cells, and downregulated CD44 expression after the addition of the antioxidant N-acetyl cysteine. Our results indicate that CD44 plays a role in the EMT phenotype of CSCs in NPC, and suggest its involvement in EMT-associated ROS production. These findings might facilitate the development of a novel therapy for the prevention of NPC recurrence and metastasis.

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International Journal of Molecular Sciences

Tập 14 Số 7

13266-13281

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