Bioassay‐guided fractionation of lemon balm (Melissa officinalisL.) using anin vitromeasure of GABA transaminase activity

Phytotherapy Research - Tập 23 Số 8 - Trang 1075-1081 - 2009
Rosalie Awad1, Asim Muhammad2, Tony Durst2, Vance L. Trudeau1, John T. Arnason1
1Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.
2Ottawa‐Carleton Institute of Chemistry, University of Ottawa, Ottawa, Ontario, Canada

Tóm tắt

AbstractA novel pharmacological mechanism of action for the anxiolytic botanicalMelissa officinalisL. (lemon balm) is reported. The methanol extract was identified as a potentin vitroinhibitor of rat brain GABA transaminase (GABA‐T), an enzyme target in the therapy of anxiety, epilepsy and related neurological disorders. Bioassay‐guided fractionation led to the identification and isolation of rosmarinic acid (RA) and the triterpenoids, ursolic acid (UA) and oleanolic acid (OA) as active principles. Phytochemical characterization of the crude extract determined RA as the major compound responsible for activity (40% inhibition at 100 µg/mL) since it represented approximately 1.5% of the dry mass of the leaves. Synergistic effects may also play a role. Copyright © 2009 John Wiley & Sons, Ltd.

Từ khóa


Tài liệu tham khảo

10.1248/bpb.25.1485

10.1136/jnnp.74.7.863

al‐Sereiti MR, 1999, Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials, Indian J Exp Biol, 37, 124

10.1177/0269881103017002004

10.1139/Y07-083

10.4088/JCP.v63n0703

10.1038/sj.mp.4001362

10.1016/S0378-8741(02)00379-3

Chung YK, 2001, Inhibitory effect of ursolic acid purified from Origanum majorana L. on the acetylcholinesterase, Mol Cells, 11, 137, 10.1016/S1016-8478(23)17016-6

10.1211/0022357023321

10.1093/jn/133.5.1286

Grieve M, 1998, A Modern Herbal.

Hubert JJ, 1990, PROBIT: A Program in PASCAL for Univariate Probit Analysis with Exact Confidence Limits for LC50.

10.1016/j.jep.2005.10.015

10.1002/ptr.1787

10.1097/01.psy.0000132877.72833.71

10.1016/S0091-3057(02)00777-3

10.1038/sj.npp.1300230

10.1016/0378-8741(95)90032-2

10.1021/np070351s

10.1021/jf030698a

10.1080/10715760500473834

10.1055/s-2002-20247

Novotny L, 2001, Ursolic acid: an anti‐tumorigenic and chemopreventive activity. Minireview, Neoplasma, 48, 241

Patora J, 2003, Variability in the content and composition of essential oil from lemon balm (Melissa officinalis L.) cultivated in Poland, Acta Pol Pharm, 60, 395

10.1016/j.phrs.2005.03.003

10.1016/S0031-9422(02)00513-7

10.1016/j.jep.2004.10.023

10.1016/j.neulet.2004.03.011

Small E, 1997, Culinary Herbs.

10.1055/s-2006-960042

10.1016/S0014-2999(02)02037-X

10.1007/s00213-002-1253-5

10.1002/ptr.2076

10.1080/J157v05n04_08

10.1016/S0378-8741(99)00113-0

10.1016/S0021-9673(02)01712-0

Thông tin
Thông tin xuất bản

Phytotherapy Research

Tập 23 Số 8

1075-1081

Thông tin tác giả