Chromosome‐Determined Zinc‐Responsible Operon czr in Staphylococcus aureus Strain 912

Microbiology and Immunology - Tập 43 Số 2 - Trang 115-125 - 1999
Makoto Kuroda1, Junya Kuroda1, Toshiko Ohta1
1Department of Microbiology, Institute of Basic Medical Sciences, and College of Medical Technology University of Tsukuba Tsukuba Ibareiki 305‐8577 Japan

Tóm tắt

Abstract

A novel operon, czrAB (zinc‐responsible genes), was identified in the chromosome of Staphylococcus aureus. The operon consists of two genes, czrA and czrB. The czrA gene, coding for an 11.5 kDa protein, was homologous to cadC, arsR of S. aureus plasmid pI258 and smtB of Synechococcus PCC7942. The czrB, coding for a 36 kDa membrane spanning protein, was homologous to the czcD gene, cobalt, zinc and the cadmium‐resistant factor of Bacillus subtilis and Alcaligenes eutrophus. In the presence of zinc (0.1‐10 mM), the transcription of czrAB was enhanced in a concentration‐dependent manner. Other heavy metals, such as cobalt, copper, manganese and nickel showed no effect on czrAB expression. The disruptant of the czrB gene became sensitive to zinc ion (MIC, 2 mM; MBC, 10 mM), and the complementation with the plasmid recovered the resistance to zinc at the same concentration as a parental strain (MIC, 5 mM; MBC, 20 mM). The disruptant accumulated intracellular zinc up to 0.4 mg per g dry weight of the organism, while that of the parental strain was 0.25 mg per g dry weight. The findings indicated that the novel operon czrAB should play a role in the transportation of zinc across the cell membrane to maintain the proper intracellular concentration.

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Microbiology and Immunology

Tập 43 Số 2

115-125

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