3,5-Diiodo-l-thyronine modulates the expression of genes of lipid metabolism in a rat model of fatty liver

Journal of Endocrinology - Tập 212 Số 2 - Trang 149-158 - 2012
Elena Grasselli1, Adriana Voci2, Ilaria Demori2, Laura Canesi2,3, Rita De Matteis4, Fernando Goglia5, Antonia Lanni6, Gabriella Gallo2, Laura Vergani2,3
1DIPTERIS, Università di Genova, Genova, Italy
2DIPTERIS, Universita `di Genova, Genova, Italy
3Istituto Nazionale Biostrutture e Biosistemi (INBB), Rome, Italy
4Dipartimento di Scienze Biomolecolari, Universita `di Urbino, Urbino, Italy
5Dipartimento di Scienze Biologiche ed Ambientali, Universita `del Sannio, Benevento, Italy
6Dipartimento di Scienze della Vita, Seconda Universita `di Napoli, Caserta, Italy

Tóm tắt

Recent reports demonstrated that 3,5-diiodo-l-thyronine (T2) was able to prevent lipid accumulation in the liver of rats fed a high-fat diet (HFD). In this study, we investigated how the rat liver responds to HFD and T2treatment by assessing the transcription profiles of some genes involved in the pathways of lipid metabolism: oxidation, storage and secretion. The mRNA levels of the peroxisome proliferator-activated receptors (PPARα, PPARγ and PPARδ), and of their target enzymes acyl-CoA oxidase and stearoyl-CoA desaturase were evaluated by real-time RT-PCR. Moreover, the expression of the adipose triglyceride lipase involved in lipid mobilisation, of the main PAT proteins acting in lipid droplet (LD) turnover, and of apoprotein B (apo B), the major protein component of very low-density lipoproteins (VLDLs) were analysed. Overall, our data demonstrated that T2administration to HFD rats counteracts most of the hepatic transcriptional changes that occurred in response to the excess exogenous fat. In particular, our results suggest that T2may prevent the pathways leading to lipid storage in LDs, promote the processes of lipid mobilisation from LDs and secretion as VLDL, in addition to the stimulation of pathways of lipid oxidation. In conclusion, our findings might give an insight into the mechanisms underlying the anti-steatotic ability of T2and help to define the potential therapeutic role of T2for preventing or treating liver steatosis.

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Tài liệu tham khảo

2004, Journal of Biological Chemistry, 279, 10070, 10.1074/jbc.M308522200

Balasubramanian, 2010, Diabetic medicine : a journal of the British Diabetic Association, 27, 150, 10.1111/j.1464-5491.2009.02906.x

Bickel, 2009, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology, 1791, 419, 10.1016/j.bbalip.2009.04.002

Bogacka, 2005, Diabetes, 54, 1392, 10.2337/diabetes.54.5.1392

Browning, 2004, Journal of Clinical Investigation, 114, 147, 10.1172/JCI200422422

Bustin, 2010, Methods (San Diego, Calif.), 50, 217, 10.1016/j.ymeth.2009.12.006

Castelein, 1994, Journal of Biological Chemistry, 269, 26754, 10.1016/S0021-9258(18)47083-4

2010, The American Journal of Clinical Nutrition, 91, 262S, 10.3945/ajcn.2009.28449D

2005, Journal of Clinical Investigation, 115, 2244, 10.1172/JCI24130

Cimmino, 1996, Journal of Endocrinology, 149, 319, 10.1677/joe.0.1490319

Collino, 2010, British journal of pharmacology, 160, 1892, 10.1111/j.1476-5381.2010.00671.x

Dalen, 2007, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology, 1771, 210, 10.1016/j.bbalip.2006.11.011

2006, 130, 207

Delerive, 2001, Journal of Endocrinology, 169, 453, 10.1677/joe.0.1690453

2008, Endocrinology, 149, 942, 10.1210/en.2007-1713

Duncan, 2007, Annual review of nutrition, 27, 79, 10.1146/annurev.nutr.27.061406.093734

Duval, 2007, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology, 1771, 961, 10.1016/j.bbalip.2007.05.003

Evans, 2004, Nature medicine, 10, 355, 10.1038/nm1025

2003, Journal of Biological Chemistry, 278, 34268, 10.1074/jbc.M300043200

2005, 70, 164

Grasselli, 2008, Experimental Biology and Medicine, 233, 549, 10.3181/0710-RM-266

Grasselli, 2010, International journal of molecular medicine, 25, 505

Grasselli, 2011, Journal of hepatology, 54, 1230, 10.1016/j.jhep.2010.09.027

2011, Journal of Endocrinology, 210, 59, 10.1530/JOE-11-0074

Haemmerle, 2006, Science, 312, 734, 10.1126/science.1123965

Hsu, 1981, Journal of Histochemistry & Cytochemistry, 29, 577, 10.1177/29.4.6166661

2005, Journal of Biological Chemistry, 280, 27466, 10.1074/jbc.M503836200

Jokinen, 1994, Journal of Biological Chemistry, 269, 26411, 10.1016/S0021-9258(18)47209-2

2008, PNAS, 105, 94, 10.1073/pnas.0708579105

Kapadia, 2008, Frontiers in bioscience : a journal and virtual library, 13, 1813, 10.2741/2802

2007, American Journal of Physiology - Endocrinology And Metabolism, 293, E1736, 10.1152/ajpendo.00122.2007

2010, The Journal of Lipid Research, 51, 468, 10.1194/jlr.R000034

Koopman, 2001, Histochemistry and cell biology, 116, 63, 10.1007/s004180100297

Lanni, 1994, Molecular and cellular endocrinology, 99, 89, 10.1016/0303-7207(94)90150-3

2005, The FASEB Journal, 10.1096/fj.05-3977fje

1998, The Biochemical journal, 330, 521, 10.1042/bj3300521

Lombardi, 2007, FEBS Letters, 581, 5911, 10.1016/j.febslet.2007.11.073

2006, Arteriosclerosis, Thrombosis, and Vascular Biology, 26, 1566, 10.1161/01.ATV.0000223345.11820.da

Mollica, 2009, Journal of hepatology, 51, 363, 10.1016/j.jhep.2009.03.023

Moreno, 1997, The Journal of Physiology, 505, 529, 10.1111/j.1469-7793.1997.529bb.x

Motojima, 1998, Journal of Biological Chemistry, 273, 16710, 10.1074/jbc.273.27.16710

Musso, 2009, Progress in lipid research, 48, 1, 10.1016/j.plipres.2008.08.001

Nishikawa, 1983, Endocrinologia japonica, 30, 167, 10.1507/endocrj1954.30.167

Olofsson, 2009, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology, 1791, 448, 10.1016/j.bbalip.2008.08.001

Ong, 2011, Hepatology (Baltimore, Md.), 53, 116, 10.1002/hep.24006

Pfaffl, 2001, Nucleic Acids Research, 29, e45, 10.1093/nar/29.9.e45

2010, NUCLEAR RECEPTOR SIGNALING, 8, 002e

Reddy, 2001, American Journal of Physiology - Gastrointestinal and Liver Physiology, 281, G1333, 10.1152/ajpgi.2001.281.6.G1333

2008, Journal of Biological Chemistry, 283, 13087, 10.1074/jbc.M800533200

Rogue, 2011, Toxicology and applied pharmacology, 254, 18, 10.1016/j.taap.2011.04.005

Romero, 2009, Bioscience reports, 29, 237, 10.1042/BSR20080089

2005, American Journal of Physiology - Endocrinology And Metabolism, 288, E1195, 10.1152/ajpendo.00513.2004

Schoonjans, 1996, The EMBO Journal, 15, 5336, 10.1002/j.1460-2075.1996.tb00918.x

Serviddio, 2008, European journal of clinical investigation, 38, 245, 10.1111/j.1365-2362.2008.01936.x

2010, MOLECULAR BIOSYSTEMS, 6, 2256, 10.1039/c0mb00040j

2006, Journal of Biological Chemistry, 281, 33066, 10.1074/jbc.M607148200

Straus, 2007, Trends in immunology, 28, 551, 10.1016/j.it.2007.09.003

Sun, 2010, Molecular biology reports, 37, 485, 10.1007/s11033-009-9672-6

Unger, 2010, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology, 1801, 209, 10.1016/j.bbalip.2009.10.006

Vance, 1990, Annual review of nutrition, 10, 337, 10.1146/annurev.nu.10.070190.002005

2011, RESEARCH IN AUTISM SPECTRUM DISORDERS, 5, 286, 10.1016/j.rasd.2010.04.010

Wanders, 2010, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology, 1801, 272, 10.1016/j.bbalip.2010.01.001

2010, Molecular Biology of the Cell, 21, 1991, 10.1091/mbc.E09-05-0364

2001, Journal of Biological Chemistry, 276, 5101, 10.1074/jbc.M006775200

2006, Journal of Biological Chemistry, 281, 14232, 10.1074/jbc.M601682200

2009, The Journal of Lipid Research, 50, 3, 10.1194/jlr.R800031-JLR200

Zimmermann, 2009, Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology, 1791, 494, 10.1016/j.bbalip.2008.10.005

Zivkovic, 2007, The American Journal of Clinical Nutrition, 86, 285, 10.1093/ajcn/86.2.285

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Thông tin xuất bản

Journal of Endocrinology

Tập 212 Số 2

149-158

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