Glucose stimulates the activation domain potential of the PDX‐1 homeodomain transcription factor
Tóm tắt
Glucose‐stimulated expression of the insulin gene in β cells is mediated by the PDX‐1 transcription factor. In this report, we show that stimulation results from effects on activation and DNA‐binding potential. Thus, glucose specifically stimulated expression in MIN6 β cells from chimeras of PDX‐1 and the GAL4 DNA‐binding domain which spanned the N‐terminal PDX‐1 activation domain located between amino acids 1 to 79. GAL4:PDX activity was induced over physiological glucose concentrations and was also regulated by effectors of this response. The level of endogenous PDX‐1 binding and phosphorylation were also induced under these conditions. We discuss how changes in PDX‐1 phosphorylation may influence activity in glucose‐treated β cells.
Tài liệu tham khảo
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