Impaired phagocytic mechanism in annexin 1 null macrophages

British Journal of Pharmacology - Tập 148 Số 4 - Trang 469-477 - 2006
Simon Yona1, Sigrid E.M. Heinsbroek2, Leanne Peiser2, Siamon Gordon2, Mauro Perretti1, Roderick J. Flower1
1Department of Biochemical Pharmacology, The William Harvey Research Institute, St Bartholomew's and the Royal London School of Medicine, University of London, Charterhouse Square, London EC1M 6BQ
2Sir William Dunn School of Pathology, Oxford University, Oxford, OX1 3RE

Tóm tắt

The role of the anti‐inflammatory protein annexin‐A1 (Anx‐A1) in the phagocytic process has been investigated using a murine bone marrow culture‐derived macrophage model from Anx‐A1+/+ and Anx‐A1−/− mice.

Macrophages prepared from Anx‐A1−/− mice exhibited a reduced ingestion of zymosan, Neisseria meningitidis or sheep red blood cells, when compared to Anx‐A1+/+ cells and in the case of zymosan this effect was also mirrored by a reduced clearance in vivo when particles were injected into the peritoneal cavity of Anx‐A1−/− mice.

The ablation of the Anx‐A1 gene did not cause any apparent cytoskeletal defects associated with particle ingestion but the cell surface expression of the key adhesion molecule CD11b was depressed in the Anx‐A1−/− cells providing a possible explanation for the attenuated phagocytic potential of these cells.

The production of the cytokines TNFα and IL‐6 was increased in Anx‐A1−/− macrophages following phagocytosis of all types of particle.

British Journal of Pharmacology (2006) 148, 469–477. doi:10.1038/sj.bjp.0706730

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British Journal of Pharmacology

Tập 148 Số 4

469-477

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