Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca
Tóm tắt
Protoplasts of soybean and N. glauca were induced to fuse with polyethylene glycol (PEG 1540). Up to 39% of the protoplasts in the treated population were heterokaryocytes. When the heterokaryocytes were isolated and individually cultivated they divided indefinitely and each produced many millions of cells within 2–3 months. The chromosomal behaviour of soybean and N. glauca in the hybrids were not synchronous in the first few cell generations and the chromosomes of N. glauca had a tendency to stick together and break into pieces. However, some of the N. glauca chromosomes were still retained in the somatic hybrids after 6 months of culturing. The chromosomes of the N. glauca were reconstructed in such a way that in the later cell generations, the movement of the N. glauca chromosomes were in synchrony with the soybean chromosomes.
Tài liệu tham khảo
Constabel, F., Dudits, D., Gamborg, O.L., Kao, K.N.: Nuclear fusion in intergeneric heterokaryons. A. Note. Canad. J. Bot. 53, 2092–2095 (1975)
Dudits, D., Kao, K.N., Constabel, F., Gamborg, O.L.: Fusion of carrot and barley protoplasts and division of heterokaryocytes. Canad. J. Genet. Cytol. 18, 263–269 (1976)
Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension culture of soybean root cells. Exp. Cell Res. 50, 151–158 (1968)
Goodspeed, T.H.: The genus Nicotiana, pp. 536. Waltham. Mass.: Chromica Botanica 1954
Harris, H.: Cell fusion, pp. 108. The Dunham Lectures. Oxford: Clarendon Press 1970
Kao, F.-T., Jones, C., Puck, T.T.: Genetics of somatic mammalian cells: Genetic immunologic and biochemical analysis with Chinese hamster cell hybrids containing selected human chromosomes. Proc. nat. Acad. Sci. (Wash.) 73, 193–197 (1975)
Kao, K.N.: A method for fusion of plant protoplasts with polyethylene glycol. In: Plant tissue culture methods. Ed. Gamborg, O.L. and Wetter, L.R., pp. 22–27. Saskatoon, Canada: Prairie Regional Laboratory, National Research Council 1975a
Kao, K.N.: A nuclear staining method for plant protoplasts. In: Plant tissue culture methods. Ed. Gamborg, O.L. and Wetter, L.R., pp. 60–62. Saskatoon, Canada: Prairie Regional Laboratory, National Research Council 1975b
Kao, K.N.: A chromosomal staining method for cultured cells. In: Plant tissue culture methods. Ed. Gamborg, O.L. and Wetter, L.R., pp. 63–64. Saskatoon, Canada: Prairie Regional Laboratory, National Research Council 1975c
Kao, K.N., Constabel, F., Michayluk, M.R., Gamborg, O.L.: Plant protoplant fusion and growth of intergeneric hybrid cells. Planta (Berl.) 120, 215–227 (1974)
Kao, K.N., Miller, R.A., Gamborg, O.L., Harvey, B.L.: Variations in chromosome number and structure in plant cells grown in suspension cultures. Canad. J. Genet. Cytol. 12, 297–301 (1970)
Michayluk, M.R., Kao, K.N.: A comparative study of sugars and sugar alcohols on cell regeneration and sustained cell division in plant protoplasts. Z. Pflanzenphysiol. 75, 181–185 (1975)
Wetter, L.R.: Isoenzyme patterns in soybean-Nicotiana somatic hybrid cell lines. Molec. gen. Genet. 150, 231–235 (1977)