Growth and death rates of bovine embryonic kidney cells in turbulent microcarrier bioreactors
Tóm tắt
Bead-bead collisions have been characterized using the velocity of the smallest turbulent eddies to calculate a turbulent collision severity (defined as the energy of collisions times their frequency), but a shear-based collision mechanism with a different dependence on the system variables is also applicable. This shearbased mechanism and the ratio of smallest eddy size to microcarrier diameter can explain the beneficial effects of both smaller diameter microcarriers and higher viscosity of the medium on the growth rate of bovine embryonic kidney cells. Death rates of these cells have also been measured at several levels of agitation. The decrease in apparent growth rate from increasing agitation is caused both by a higher rate of cell death as well as a lower intrinsic growth rate.
Tài liệu tham khảo
Bethea, R. M.; Duran, B. S.; Boullion, T. L.: Statistical methods for engineers and scientists. New York: Dekker 1985
Cherry, R. S.; Papoutsakis, E. T.: Hydrodynamic effects on cells in agitated tissue culture reactors. Bioproc. Eng. 1 (1986) 29–41
Cherry, R. S.: Hydrodynamic mechanisms of cell damage in microcarrier bioreactors. Ph.D. dissertation, Dept. of Chemical Engineering, Rice University, Houston, TX. USA (August, 1987)
Cherry, R. S.; Papoutsakis, E. T.: Physical mechanisms of cell damage in microcarrier cell culture bioreactors. Biotechnol. Bioeng., in press (1988)
Churchill, S. W.: The interpretation and use of rate data: the rate concept. New York: McGraw-Hill 1974
Croughan, M. S.: Hamel, J.-F.; Wang, D. I. C.: Hydrodynamic effects on animal cells grown in microcarrier cultures. Biotechnol. Bioeng. 29 (1987) 130–141
Croughan, M. S.; Hamel, J.-F.; Wang, D. I. C.: Effects of microcarrier concentration in animal cell culture. Biotechnol. Bioeng., in press (1988)
Delichatsios, M. A.: Particle coagulation in steady turbulent flows: Application to smoke aging. J. Col. Interface Sci. 78 (1980) 163–174
Feder, J.; Tolbert, W. R.: The large scale culture of mammalian cells. Sci. Am 248 (1983) 36–43
Fleischaker, J. A., Jr.; Sinskey, A. J.: Oxygen demand and supply in cell culture. Eur. J. Appl. Microbiol. 12 (1981) 193–197
Glacken, M. W.; Fleishaker, R. J.; Sinskey, A. J.: Mammalian cell culture: engineering principles and scaleup. Trends Biotechnol. 1 (1983) 102–108
Hing, W. F.: Production of Autographa Californica nuclear polyhedrosis virus in cells from large-scale suspension cultures. In: Kurstak, E. (Ed.): Microbial and Viral Pesticides. New York: Marcell Dekker 1982
Hinze, J. O.: Turbulent fluid and particle interaction. Prog. Heat Mass Transfer 6 (1971) 433–452
Hu, W. S.: Quantitative and mechanistic analysis of mammalian cell cultivation on microcarriers. Ph.D. thesis, Massachusetts Institute of Technology, Cambridge, MA (Sept. 1983)
Lehmann, J.; Piehl, G. W.; Shulz, R.: Bubble free cell culture aeration with porous moving membranes. Develop. biol. Standard. 66 (1987) 227–240
Margaritis, A.; Wallace, J. B.: Novel bioreactor systems and their applications. Bio/Technology 2 (1984) 447–453
Meiselman, H. J.; Cokelet, G. R.: Blood rheology. Adv. Microcirc. 5 (1973) 32–61
Midler, M.; Finn, R. K.: A model system for evaluating shear in the design of stirred fermenters. Biotechnol. Bioeng. 8 (1966) 72–84
Mizrahi, A.: Oxygen in human lymphoblastoid cell line cultures and effect of polymers in agitated and aerated cultures. Develop. biol. Standard. 55 (1984) 93–102
Nagata, S.: Mixing — principles and applications. New York: Halsted Press 1975
Oldshue, J. Y.: Fluid mixing technology. New York: McGrawHill 1983
Schulz, R.; Krafft, H.; Lehmann, J.: Experiments with a new type of microcarrier. Biotech. Letters 8 (1986) 557–560
Sinskey, A. J.; Fleishaker, R. J.; Tyo, M. A.; Giard, D.-J.; Wang, D. I. C.: Production of cell-derived products: virus and interferon. Ann. N. Y. Acad. Sci. 369 (1981) 47–59
Varani, J.; Dame, M.; Beals, T. F.; Wass, J. A.: Growth of three established cell lines on glass microcarriers. Biotechnol. Bioeng. 25 (1983) 1359–1372