Granulocyte‐macrophage progenitor cell preservation at 4°C

British Journal of Haematology - Tập 53 Số 1 - Trang 49-54 - 1983
Alain Delforge1, E Rongé-Collard1, Pierre Stryckmans1, Theodore E. Spiro2, Marie A. Malarme1
1Servicede Médecine Interne et Laboratoire d'Investigation Clinique Henri Tagnon, Institut Jules Bordet, Rue Héger‐Bordet, 1000 Brussels, Belgium
2The Upjohn Company, Kalamazoo, Michigan, U.S.A.

Tóm tắt

Summary. Eighteen bone marrows collected from patients without haematological diseases and from normal subjects were tested for the effect of 4 d storage at 4°C on CFU‐C growth. Results indicate that unfractionated bone marrow cells may be stored at 4°C for 4 d with 97%± 8 SEM recovery of the CFU‐C evaluated by the agar culture assay. On the other hand, the same preservation procedure on peripheral blood CFU‐C of 13 normal subjects yielded only 5%± 2 SEM recovery of in vitro growth capacity.

The present results have practical implications. They might be exploited to preserve bone marrow CFU‐C for transplantation therapy or laboratory investigation. In contrast this single preservation procedure seems not appropriate for preserving blood CFU‐C.

Từ khóa


Tài liệu tham khảo

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Thông tin
Thông tin xuất bản

British Journal of Haematology

Tập 53 Số 1

49-54

Thông tin tác giả